Strain identifier
BacDive ID: 14313
Type strain: 
Species: Borrelia lanei
Strain Designation: CA28, CA28-91
Strain history: CIP <- 2006, D. Postic, Inst. Pasteur, Paris, France: strain CA 28 Borrelia burgdorferi <- T. Schwan
NCBI tax ID(s): 373540 (species)
version 8.1 (current version)
General
@ref: 7322
BacDive-ID: 14313
DSM-Number: 17992
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, motile, helical-shaped
description: Borrelia lanei CA28 is a microaerophile, mesophilic, motile bacterium that was isolated from tick Ixodes pacificus.
NCBI tax id
- NCBI tax id: 373540
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7322 | <- D. Postic, CIP; CA28 <- T.G. Schwan, Natl. Inst. Allergy and Infectious Diseases, Hamilton, Montana, USA; CA28-91 <- CDHS, California Dept. of Health Services |
| 120553 | CIP <- 2006, D. Postic, Inst. Pasteur, Paris, France: strain CA 28 Borrelia burgdorferi <- T. Schwan |
doi: 10.13145/bacdive14313.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Borreliaceae
- genus: Borrelia
- species: Borrelia lanei
- full scientific name: Borrelia lanei Margos et al. 2017
synonyms
- @ref: 20215
- synonym: Borreliella lanei
@ref: 7322
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Borreliaceae
genus: Borrelia
species: Borrelia lanei
full scientific name: Borrelia lanei Margos et al. 2017
strain designation: CA28, CA28-91
type strain: yes
Morphology
cell morphology
- @ref: 43525
- cell length: 20 µm
- cell width: 0.2 µm
- cell shape: helical-shaped
- motility: yes
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 36785 | MEDIUM 83 - for Borrelia | yes | BSK-H medium (1000.000 ml) | |
| 7322 | BSK-MEDIUM (DSMZ Medium 403) | yes | Name: BSK-MEDIUM (DSMZ Medium 403) Composition: Bovine serum albumin 35.0245 g/l Gelatine 9.79706 g/l HEPES buffer 4.19874 g/l Proteose peptone no. 2 3.49895 g/l Glucose 2.09937 g/l Proteose yeastolate 0.69979 g/l Tryptone peptone 0.69979 g/l Na-pyruvate 0.559832 g/l Sodium citrate 0.489853 g/l N-Acetylglucosamine 0.279916 g/l MgCl2 x 6 H2O 0.209937 g/l L-Glutamine 0.069979 g/l Double distilled water CMRL 1066 Rabbit serum | https://mediadive.dsmz.de/medium/403 |
| 120553 | CIP Medium 83 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=83 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 36785 | positive | growth | 34 | mesophilic |
| 43525 | positive | optimum | 33-34 | mesophilic |
| 7322 | positive | growth | 35 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 7322 | microaerophile |
| 43525 | microaerophile |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | geographic location | country | origin.country | continent | isolation date |
|---|---|---|---|---|---|---|---|
| 7322 | tick Ixodes pacificus | Ixodes pacificus | California | USA | USA | North America | |
| 43525 | a pool of 10 questing I. pacificus ticks | Kern Co., California | USA | USA | North America | 1991 | |
| 120553 | Ixodes pacificus | California | United States of America | USA | North America | 1993 |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Tick
taxonmaps
- @ref: 69479
- File name: preview.99_427.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15870;96_290;97_320;98_355;99_427&stattab=map
- Last taxonomy: Borreliella
- 16S sequence: AJ224131
- Sequence Identity:
- Total samples: 1677
- soil counts: 126
- aquatic counts: 378
- animal counts: 1133
- plant counts: 40
Safety information
risk assessment
- @ref: 120553
- biosafety level: 2
- biosafety level comment: Risk group (French classification)
Sequence information
16S sequences
- @ref: 7322
- description: Borrelia burgdorferi sensu lato, 16S rRNA gene, isolate CA28
- accession: AJ224131
- length: 1467
- database: ena
- NCBI tax ID: 373540
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Borreliella lanei DSM 17992 | GCA_014205895 | scaffold | ncbi | 373540 |
| 66792 | Borrelia lanei strain DSM 17992 | 373540.3 | wgs | patric | 373540 |
| 66792 | Borreliella lanei DSM 17992 | 2861253713 | draft | img | 373540 |
GC content
- @ref: 43525
- GC-content: 27
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 87.71 | no |
| gram-positive | no | 93.699 | no |
| anaerobic | no | 92.485 | yes |
| aerobic | no | 97.484 | yes |
| halophile | yes | 64.824 | no |
| spore-forming | no | 97.493 | no |
| glucose-util | yes | 78.443 | no |
| motile | yes | 88.376 | yes |
| glucose-ferment | yes | 68.453 | no |
| thermophile | no | 85.305 | yes |
External links
@ref: 7322
culture collection no.: DSM 17992, CIP 109135
straininfo link
- @ref: 83464
- straininfo: 361802
literature
- topic: Phylogeny
- Pubmed-ID: 28884668
- title: Borrelia lanei sp. nov. extends the diversity of Borrelia species in California.
- authors: Margos G, Fedorova N, Kleinjan JE, Hartberger C, Schwan TG, Sing A, Fingerle V
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002214
- year: 2017
- mesh: Animals, Bacterial Typing Techniques, Base Composition, Borrelia burgdorferi Group/*classification/genetics/isolation & purification, California, DNA, Bacterial/genetics, Genes, Bacterial, Ixodes/*microbiology, Multilocus Sequence Typing, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Transcriptome
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 7322 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17992) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17992 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 36785 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6843 | ||||
| 43525 | Gabriele Margos, Natalia Fedorova, Joyce E. Kleinjan, Christine Hartberger, Tom G. Schwan, Andreas Sing, Volker Fingerle | 10.1099/ijsem.0.002214 | Borrelia lanei sp. nov. extends the diversity of Borrelia species in California | IJSEM 67: 3872-3876 2017 | 28884668 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 83464 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID361802.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 120553 | Curators of the CIP | Collection of Institut Pasteur (CIP 109135) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109135 |