Archive BacDiveID:10.13145/bacdive14310.20230509.8.1

Strain identifier

BacDive ID: 14310

Type strain:

Species: Borrelia californiensis

Strain Designation: CA446, CA 446

Strain history: CIP <- 2006, <- D. Postic, Inst. Pasteur, Paris, France: strain CA 446 <- R. Lane

NCBI tax ID(s): 373543 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 7319

BacDive-ID: 14310

DSM-Number: 17989

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, motile, helical-shaped, animal pathogen

description: Borrelia californiensis CA446 is a microaerophile, mesophilic, motile animal pathogen that was isolated from Kangaroo rat, Dipodomys californicus.

NCBI tax id

NCBI tax id: 373543
Matching level: species

strain history

@ref	history
7319	<- D. Postic; CA446 {2006}
117103	CIP <- 2006, <- D. Postic, Inst. Pasteur, Paris, France: strain CA 446 <- R. Lane

doi: 10.13145/bacdive14310.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/spirochaetota
domain: Bacteria
phylum: Spirochaetota
class: Spirochaetia
order: Spirochaetales
family: Borreliaceae
genus: Borrelia
species: Borrelia californiensis
full scientific name: Borrelia californiensis Margos et al. 2016
synonyms
- @ref: 20215
- synonym: Borreliella californiensis

@ref: 7319

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Borreliaceae

genus: Borrelia

species: Borrelia californiensis

full scientific name: Borrelia californiensis Margos et al. 2016

strain designation: CA446, CA 446

type strain: yes

Morphology

cell morphology

@ref	cell length	cell width	cell shape	motility	gram stain	confidence
43710	20 µm	0.2 µm	helical-shaped	yes
69480					negative	99.849

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
7319	BSK-MEDIUM (DSMZ Medium 403)	yes	https://mediadive.dsmz.de/medium/403	Name: BSK-MEDIUM (DSMZ Medium 403) Composition: Bovine serum albumin 35.0245 g/l Gelatine 9.79706 g/l HEPES buffer 4.19874 g/l Proteose peptone no. 2 3.49895 g/l Glucose 2.09937 g/l Proteose yeastolate 0.69979 g/l Tryptone peptone 0.69979 g/l Na-pyruvate 0.559832 g/l Sodium citrate 0.489853 g/l N-Acetylglucosamine 0.279916 g/l MgCl2 x 6 H2O 0.209937 g/l L-Glutamine 0.069979 g/l Double distilled water CMRL 1066 Rabbit serum
41876	MEDIUM 83 - for Borrelia	yes		BSK-H medium (1000.000 ml)
43710	BSK medium	yes
117103	CIP Medium 83	yes	https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=83

culture temp

@ref	growth	type	temperature	range
7319	positive	growth	35	mesophilic
41876	positive	growth	34	mesophilic
43710	positive	optimum	33-34	mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance
7319	microaerophile
43710	microaerophile

spore formation

@ref	spore formation	confidence
69481	no	100
69480	no	99.992

Isolation, sampling and environmental information

isolation

@ref	sample type	host species	geographic location	country	origin.country	continent	isolation date
7319	Kangaroo rat, Dipodomys californicus	Dipodomys californicus	California	USA	USA	North America
43710	Ear-punch biopsy excised from a male D. californicus		California	USA	USA	North America	1995-11
117103	Dipodomys californicus		California	United States of America	USA	North America	1992

isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Rodentia (Other)
#Infection	#Patient	#Biopsy

taxonmaps

@ref: 69479
File name: preview.99_427.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15870;96_290;97_320;98_355;99_427&stattab=map
Last taxonomy: Borreliella
16S sequence: AJ224130
Sequence Identity:
Total samples: 1677
soil counts: 126
aquatic counts: 378
animal counts: 1133
plant counts: 40

Safety information

risk assessment

@ref	pathogenicity animal	biosafety level	biosafety level comment
7319	yes, in single cases	1	Risk group (German classification)
117103		2	Risk group (French classification)

Sequence information

16S sequences

@ref: 7319
description: Borrelia burgdorferi sensu lato, 16S rRNA gene, isolate CA446
accession: AJ224130
length: 1467
database: ena
NCBI tax ID: 373543

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Borreliella californiensis DSM 17989	GCA_014205885	scaffold	ncbi	373543
66792	Borreliella californiensis strain DSM 17989	373543.8	wgs	patric	373543
66792	Borrelia californiensis DSM 17989	2861252286	draft	img	373543

GC content

@ref: 7319
GC-content: 27

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	100	no
flagellated	no	87.413	no
gram-positive	no	92.941	no
anaerobic	no	93.08	yes
aerobic	no	97.533	yes
halophile	yes	65.22	no
spore-forming	no	97.309	no
glucose-util	yes	77.988	no
thermophile	no	87.942	no
motile	yes	88.427	yes
glucose-ferment	yes	68.151	no

External links

@ref: 7319

culture collection no.: DSM 17989, ATCC BAA 2689, CIP 109133

straininfo link

@ref: 83463
straininfo: 361803

literature

topic: Phylogeny
Pubmed-ID: 26813420
title: Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles.
authors: Margos G, Lane RS, Fedorova N, Koloczek J, Piesman J, Hojgaard A, Sing A, Fingerle V
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijsem.0.000897
year: 2016

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
7319	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17989)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-17989
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41876	Curators of the CIP		https://brclims.pasteur.fr/brcWeb/souche/detail/1/6841
43710	Gabriele Margos, Robert S. Lane, Natalia Fedorova, Johannes Koloczek, Joseph Piesman, Andrias Hojgaard, Andreas Sing, Volker Fingerle		10.1099/ijsem.0.000897	Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles	IJSEM 66: 1447-1452 2016	26813420
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
83463	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID361803.1	StrainInfo: A central database for resolving microbial strain identifiers
117103	Curators of the CIP	Collection of Institut Pasteur (CIP 109133)	https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109133

General

NCBI tax id

strain history

Name and taxonomic classification

LPSN

synonyms

Morphology

cell morphology

Culture and growth conditions

culture medium

culture temp

Physiology and metabolism

oxygen tolerance

spore formation

Isolation, sampling and environmental information

isolation

isolation source categories

taxonmaps

Safety information

risk assessment

Sequence information

16S sequences

Genome sequences

GC content

Genome-based predictions

predictions

External links

straininfo link

literature

Reference