Strain identifier

BacDive ID: 14231

Type strain: No

Species: Sphingomonas melonis

Strain history: CIP <- 2003, R. Buonaurio, Perugia, Italy: strain DAPP-PG 228

NCBI tax ID(s): 152682 (species)

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General

@ref: 5358

BacDive-ID: 14231

DSM-Number: 14445

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, plant pathogen

description: Sphingomonas melonis DSM 14445 is an obligate aerobe, mesophilic, Gram-negative plant pathogen that was isolated from fruit of yellow spanish melons .

NCBI tax id

  • NCBI tax id: 152682
  • Matching level: species

strain history

@refhistory
5358<- R. Buonaurio; DAPP-PG 228
120889CIP <- 2003, R. Buonaurio, Perugia, Italy: strain DAPP-PG 228

doi: 10.13145/bacdive14231.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingomonadaceae
  • genus: Sphingomonas
  • species: Sphingomonas melonis
  • full scientific name: Sphingomonas melonis Buonaurio et al. 2002

@ref: 5358

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Sphingomonas

species: Sphingomonas melonis

full scientific name: Sphingomonas melonis Buonaurio et al. 2002

type strain: no

Morphology

cell morphology

  • @ref: 120889
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 120889
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5358REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36661MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120889CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5358positivegrowth28mesophilic
36661positivegrowth30mesophilic
120889positivegrowth25-37mesophilic
120889nogrowth5psychrophilic
120889nogrowth10psychrophilic
120889nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120889
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120889NaClpositivegrowth0 %
120889NaClnogrowth2 %
120889NaClnogrowth4 %
120889NaClnogrowth6 %
120889NaClnogrowth8 %
120889NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12088916947citrate-carbon source
1208894853esculin+hydrolysis
12088917632nitrate-reduction
12088916301nitrite-reduction
12088917632nitrate-respiration

antibiotic resistance

  • @ref: 120889
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12088935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120889oxidase-
120889beta-galactosidase+3.2.1.23
120889alcohol dehydrogenase-1.1.1.1
120889gelatinase-
120889amylase-
120889DNase-
120889caseinase-3.4.21.50
120889catalase+1.11.1.6
120889tween esterase-
120889lecithinase-
120889lipase-
120889lysine decarboxylase-4.1.1.18
120889ornithine decarboxylase-4.1.1.17
120889protease-
120889tryptophan deaminase-
120889urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120889-+++-++-+-++----++--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5358-----+-++++-+++--+-++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120889+++++--+-+++---+--+-++-+-------------------------+-----+---+-+-------------+--+++---------++-+---+-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
5358fruit of yellow spanish melons (Cucumis melo var. inodorus)Cucumis melo var. inodorusAlmeriaSpainESPEurope
120889Yellow Spanish melon fruitsSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Fruit (Seed)

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
5358yes1Risk group (German classification)
1208891Risk group (French classification)

External links

@ref: 5358

culture collection no.: DSM 14445, LMG 19485, DAPP-PG 228, CIP 107881

straininfo link

  • @ref: 83390
  • straininfo: 13323

literature

  • topic: Phylogeny
  • Pubmed-ID: 12508872
  • title: Sphingomonas melonis sp. nov., a novel pathogen that causes brown spots on yellow Spanish melon fruits.
  • authors: Buonaurio R, Stravato VM, Kosako Y, Fujiwara N, Naka T, Kobayashi K, Cappelli C, Yabuuchi E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-6-2081
  • year: 2002
  • mesh: Cucumis melo/microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fruit/microbiology, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sphingomonas/*classification/genetics/*pathogenicity, Terminology as Topic
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
5358Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14445)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14445
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36661Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5441
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
83390Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13323.1StrainInfo: A central database for resolving microbial strain identifiers
120889Curators of the CIPCollection of Institut Pasteur (CIP 107881)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107881