Strain identifier

BacDive ID: 14150

Type strain: Yes

Species: Novosphingobium hassiacum

Strain Designation: DGI2-11, W-51, W-51T

Strain history: CIP <- 2001, P. Kämpfer, Giessen Univ., Giessen, Germany: strain W-51T <- 1999, R. Witzenberger

NCBI tax ID(s): 173676 (species)

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General

@ref: 5293

BacDive-ID: 14150

DSM-Number: 14552

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Novosphingobium hassiacum DGI2-11 is a mesophilic, Gram-negative bacterium that was isolated from aerated sewage pond.

NCBI tax id

  • NCBI tax id: 173676
  • Matching level: species

strain history

@refhistory
5293<- P. Kämpfer; W-51
119848CIP <- 2001, P. Kämpfer, Giessen Univ., Giessen, Germany: strain W-51T <- 1999, R. Witzenberger

doi: 10.13145/bacdive14150.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Erythrobacteraceae
  • genus: Novosphingobium
  • species: Novosphingobium hassiacum
  • full scientific name: Novosphingobium hassiacum Kämpfer et al. 2002

@ref: 5293

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Erythrobacteraceae

genus: Novosphingobium

species: Novosphingobium hassiacum

full scientific name: Novosphingobium hassiacum Kämpfer et al. 2002

strain designation: DGI2-11, W-51, W-51T

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.992
119848negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5293REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)yeshttps://mediadive.dsmz.de/medium/830cName: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
32779MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119848CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
5293positivegrowth25mesophilic
32779positivegrowth25mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no96
69480no99.993

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119848-+++-++---++---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5293aerated sewage pondHessen, GüllGermanyDEUEurope
119848Aerated sevage pondHessenGermanyDEUEurope1999

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Environmental#Aquatic#Pond (small)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52931Risk group (German classification)
1198481Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5293
  • description: Novosphingobium hassiacum partial 16S rRNA gene
  • accession: AJ416411
  • length: 1485
  • database: ena
  • NCBI tax ID: 173676

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Novosphingobium hassiacum DSM 14552GCA_014196055scaffoldncbi173676
66792Novosphingobium hassiacum strain DSM 14552173676.3wgspatric173676
66792Novosphingobium hassiacum DSM 145522829799810draftimg173676

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
flagellatedno91.061no
gram-positiveno97.379no
anaerobicno98.36no
aerobicyes92.169no
halophileno97.069no
spore-formingno93.967no
glucose-utilyes87.772no
thermophileno98.385yes
motileyes53.195no
glucose-fermentno92.647no

External links

@ref: 5293

culture collection no.: DSM 14552, CIP 107176, CCUG 48038

straininfo link

  • @ref: 83316
  • straininfo: 100828

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12086187Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond.Kampfer P, Witzenberger R, Denner EB, Busse HJ, Neef ASyst Appl Microbiol10.1078/0723-2020-000832002Aerobiosis, Alphaproteobacteria/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Hybridization, Genetic, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Sewage/*microbiology, Species Specificity, *Water MicrobiologyGenetics
Phylogeny19196772Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle.Glaeser SP, Kampfer P, Busse HJ, Langer S, Glaeser JInt J Syst Evol Microbiol10.1099/ijs.0.65852-02009Fatty Acids/analysis, *Humic Substances, Hydrogen-Ion Concentration, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Salts, Species Specificity, Sphingomonadaceae/chemistry/*classification/genetics/ultrastructure, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitle
5293Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14552)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14552
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32779Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4582
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83316Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100828.1StrainInfo: A central database for resolving microbial strain identifiers
119848Curators of the CIPCollection of Institut Pasteur (CIP 107176)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107176