Strain identifier

BacDive ID: 141158

Type strain: Yes

Species: Paenibacillus solanacearum

Strain Designation: T16R-228

Strain history: CIP <- 2020, S-W. Kwon, Nat. Inst. Agric. Sci., Jeollabuk-do, Korea: strain T16R-228 <- H-Y. Weon

NCBI tax ID(s): 2048548 (species)

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General

@ref: 44079

BacDive-ID: 141158

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Paenibacillus solanacearum T16R-228 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from rhizosphere soil of tomato plant from a farm.

NCBI tax id

  • NCBI tax id: 2048548
  • Matching level: species

strain history

  • @ref: 122651
  • history: CIP <- 2020, S-W. Kwon, Nat. Inst. Agric. Sci., Jeollabuk-do, Korea: strain T16R-228 <- H-Y. Weon

doi: 10.13145/bacdive141158.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus solanacearum
  • full scientific name: Paenibacillus solanacearum Cho et al. 2017

@ref: 44079

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus solanacearum

strain designation: T16R-228

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
44079positive2.0-5.0 µm0.9-1.4 µmrod-shapednoperitrichous
122651negativeyes

colony morphology

  • @ref: 44079
  • colony size: 0.1 cm
  • colony color: white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

@refnamegrowthlink
44079Reasoner's 2A agar (R2A)yes
122651CIP Medium 9yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=9
122651CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
44079positivegrowth15-40
44079positiveoptimum28-30mesophilic

culture pH

@refabilitytypepH
44079positivegrowth5.0-8.0
44079positiveoptimum6.0-7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 44079
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formation
44079ellipsoidal spores formed in swollen sporangiaendosporeyes
122651yes

halophily

  • @ref: 44079
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
44079161933-hydroxybenzoate-assimilation
44079370543-hydroxybutyrate-assimilation
44079178794-hydroxybenzoate-assimilation
4407917128adipate-assimilation
4407927689decanoate-assimilation
4407916024D-mannose-assimilation
4407916988D-ribose-assimilation
4407917924D-sorbitol-assimilation
4407928087glycogen-assimilation
4407917268myo-inositol-assimilation
4407917240itaconate-assimilation
4407916977L-alanine-assimilation
4407930849L-arabinose-assimilation
4407918287L-fucose-assimilation
4407915971L-histidine-assimilation
4407917203L-proline-assimilation
4407962345L-rhamnose-assimilation
4407917115L-serine-assimilation
4407924996lactate-assimilation
4407925115malate-assimilation
4407928053melibiose-assimilation
44079506227N-acetylglucosamine-assimilation
4407918401phenylacetate-assimilation
44079potassium 2-dehydro-D-gluconate-assimilation
44079potassium 5-dehydro-D-gluconate-assimilation
4407932032potassium gluconate-assimilation
4407917272propionate-assimilation
4407932954sodium acetate-assimilation
4407953258sodium citrate-assimilation
4407962983sodium malonate-assimilation
440799300suberic acid-assimilation
4407931011valerate-assimilation
4407917234glucose-fermentation
44079casein-hydrolysis
4407917029chitin-hydrolysis
4407916991dna-hydrolysis
4407917368hypoxanthine-hydrolysis
4407928017starch-hydrolysis
4407918186tyrosine-hydrolysis
4407915318xanthine-hydrolysis
4407917634D-glucose+assimilation
4407916899D-mannitol+assimilation
4407917306maltose+assimilation
4407917814salicin+assimilation
4407917992sucrose+assimilation
4407985146carboxymethylcellulose+hydrolysis
4407953426tween 80+hydrolysis
4407917632nitrate+reduction

metabolite production

  • @ref: 44079
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
44079cytochrome oxidase-1.9.3.1
44079catalase-1.11.1.6
44079esterase (C 4)+
44079esterase Lipase (C 8)+
44079naphthol-AS-BI-phosphohydrolase+
44079beta-galactosidase+3.2.1.23
44079arginine dihydrolase-3.5.3.6
44079alkaline phosphatase-3.1.3.1
44079lipase (C 14)-
44079leucine arylamidase-3.4.11.1
44079valine arylamidase-
44079cystine arylamidase-3.4.11.3
44079trypsin-3.4.21.4
44079alpha-chymotrypsin-3.4.21.1
44079acid phosphatase-3.1.3.2
44079alpha-galactosidase-3.2.1.22
44079beta-glucuronidase-3.2.1.31
44079beta-glucosidase-3.2.1.21
44079N-acetyl-beta-glucosaminidase-3.2.1.52
44079alpha-mannosidase-3.2.1.24
44079alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    44079C15:0 anteiso59.8
    44079C17:0 anteiso4.2
    44079C14:01.7
    44079C16:012.1
    44079C16:1ω11c1.6
    44079C17:00.6
    44079C14:0 iso3.9
    44079C15:0 iso4.2
    44079C16:0 iso10.5
    44079C17:0 iso0.9
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A agar
  • incubation temperature: 28
  • library/peak naming table: TSBA60
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture temperatureisolation date
44079rhizosphere soil of tomato plant from a farmBuyeo, Chungcheongnam-doRepublic of KoreaKORAsia36.2934126.932R2A agar28
122651Environment, Soil, rhizosphere of a tomato plantBuyeo, Chungcheong ProvinceRepublic of KoreaKORAsia2017-05-15

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 122651
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 44079
  • description: 16S rRNA gene sequence
  • accession: KU379666
  • database: nuccore

GC content

  • @ref: 44079
  • GC-content: 56.8
  • method: fluorimetric

External links

@ref: 44079

culture collection no.: KACC 18654, NBRC 111896, CIP 111600

literature

  • topic: Phylogeny
  • Pubmed-ID: 29056112
  • title: Paenibacillus solanacearum sp. nov., isolated from rhizosphere soil of a tomato plant.
  • authors: Cho H, Heo J, Ahn JH, Weon HY, Kim JS, Kwon SW, Kim SJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002410
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lycopersicon esculentum/*microbiology, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44079Hayoung Cho, Jun Heo, Jae-Hyung Ahn, Hang-Yeon Weon, Jeong-Seon Kim, Soon-Wo Kwon and Soo-Jin KimPaenibacillus solanacearum sp. nov., isolated from rhizosphere soil of a tomato plant10.1099/ijsem.0.002410IJSEM 67: 5046-5050 201729056112
122651Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111600Collection of Institut Pasteur (CIP 111600)