Strain identifier

BacDive ID: 141155

Type strain: Yes

Species: Massilia neuiana

Strain Designation: PTW21

NCBI tax ID(s): 1850242 (species)

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General

@ref: 44076

BacDive-ID: 141155

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Massilia neuiana PTW21 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from riverbank wet soil.

NCBI tax id

  • NCBI tax id: 1850242
  • Matching level: species

doi: 10.13145/bacdive141155.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Massilia
  • species: Massilia neuiana
  • full scientific name: Massilia neuiana Zhao et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Telluria neuiana

@ref: 44076

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia neuiana

strain designation: PTW21

type strain: yes

Morphology

cell morphology

  • @ref: 44076
  • gram stain: negative
  • cell length: 1.6-2.2 µm
  • cell width: 0.7-1.0 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 44076
  • colony size: 1.5-2.5 mm
  • colony color: straw-yellow
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

@refnamegrowthcomposition
44076denitrification mediumyes(NH4)2SO4 0.236g, sodium citrate 0.512g, KH2PO4 0.1g, distilled water added to 1l final volume
44076Reasoner's 2A agar (R2A)yes

culture temp

@refgrowthtypetemperaturerange
44076positivegrowth10-45
44076positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
44076positivegrowth4.5-12.5alkaliphile
44076positiveoptimum9
44076positivegrowth6
44076nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 44076
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
44076NaClpositivegrowth0-2 %(w/v)
44076NaClpositiveoptimum0 %(w/v)non-halophilic
44076NaClnogrowth4 %
44076NaClnogrowth8 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4407616651(S)-lactate-assimilation
44076309162-oxoglutarate-assimilation
44076739183-O-methyl-D-glucose-assimilation
4407617128adipate-assimilation
44076645522-hydroxybutyrate-assimilation
4407627689decanoate-assimilation
4407618333D-arabitol-assimilation
4407629990D-aspartate-assimilation
440768391D-gluconate-assimilation
4407614314D-glucose 6-phosphate-assimilation
4407616899D-mannitol-assimilation
4407616024D-mannose-assimilation
44076D-saccharate-assimilation
4407616523D-serine-assimilation
4407617924D-sorbitol-assimilation
4407633984fucose-assimilation
4407616537galactarate-assimilation
4407616865gamma-aminobutyric acid-assimilation
4407632323glucuronamide-assimilation
4407617754glycerol-assimilation
4407615971L-histidine-assimilation
4407618183L-pyroglutamic acid-assimilation
4407617716lactose-assimilation
4407628053melibiose-assimilation
4407617268myo-inositol-assimilation
4407663154N-acetyl-beta-D-mannosamine-assimilation
4407628037N-acetylgalactosamine-assimilation
44076506227N-acetylglucosamine-assimilation
4407632032potassium gluconate-assimilation
4407626490quinate-assimilation
4407616634raffinose-assimilation
4407653258sodium citrate-assimilation
4407617164stachyose-assimilation
4407617992sucrose-assimilation
4407627082trehalose-assimilation
44076370543-hydroxybutyrate+assimilation
44076181014-hydroxyphenylacetic acid+assimilation
4407630089acetate+assimilation
4407613705acetoacetate+assimilation
4407617925alpha-D-glucose+assimilation
4407673706bromosuccinate+assimilation
4407617057cellobiose+assimilation
4407616947citrate+assimilation
4407618024D-galacturonic acid+assimilation
4407617634D-glucose+assimilation
4407615748D-glucuronate+assimilation
4407623652dextrin+assimilation
440765291gelatin+assimilation
4407628066gentiobiose+assimilation
4407670744glycine-proline+assimilation
4407616977L-alanine+assimilation
4407630849L-arabinose+assimilation
4407629991L-aspartate+assimilation
4407617464L-galactonic acid gamma-lactone+assimilation
4407629985L-glutamate+assimilation
4407615589L-malate+assimilation
4407662345L-rhamnose+assimilation
4407617115L-serine+assimilation
4407625115malate+assimilation
4407617306maltose+assimilation
4407674611methyl (R)-lactate+assimilation
44076320055methyl beta-D-glucopyranoside+assimilation
4407651850methyl pyruvate+assimilation
4407617309pectin+assimilation
4407618401phenylacetate+assimilation
4407617272propionate+assimilation
4407617814salicin+assimilation
4407653423tween 40+assimilation
44076casein+hydrolysis
440764853esculin+hydrolysis
440765291gelatin+hydrolysis
4407628017starch+hydrolysis
4407629016arginine+/-assimilation
4407615824D-fructose+/-assimilation
4407678697D-fructose 6-phosphate+/-assimilation
4407612936D-galactose+/-assimilation
4407615740formate+/-assimilation
4407617596inosine+/-assimilation
4407632528turanose+/-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4407671321fusidateyesyes
4407616523d-serineyesyes
4407645735troleandomycinyesyes
4407650694minocyclineyesyes
4407628001vancomycinyesyes
4407632735guanidinium chlorideyesyes
4407675273niaproofyesyes
4407675193tetrazolium violetyesyes
4407675198tetrazolium blueyesyes
44076100147nalidixic acidyesyes
4407675248potassium telluriteyesyes
4407675229sodium bromateyesyes
4407664103sodium butyrateyesyes
440766472lincomycinyesyes
4407648607lithium chlorideyesyes
44076161680aztreonamyesyes

enzymes

@refvalueactivityec
44076cytochrome oxidase+1.9.3.1
44076catalase+1.11.1.6
44076alkaline phosphatase+3.1.3.1
44076esterase Lipase (C 8)+
44076leucine arylamidase+3.4.11.1
44076valine arylamidase+
44076cystine arylamidase+3.4.11.3
44076naphthol-AS-BI-phosphohydrolase+
44076esterase (C 4)-
44076lipase (C 14)-
44076trypsin-3.4.21.4
44076alpha-chymotrypsin-3.4.21.1
44076acid phosphatase-3.1.3.2
44076alpha-galactosidase-3.2.1.22
44076beta-galactosidase-3.2.1.23
44076alpha-glucosidase-3.2.1.20
44076beta-glucosidase-3.2.1.21
44076N-acetyl-beta-glucosaminidase-3.2.1.52
44076alpha-mannosidase-3.2.1.24
44076alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

  • @ref: 44076
  • sample type: riverbank wet soil
  • geographic location: Maonan District, Maoming, Guangdong Province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 21.5932
  • longitude: 110.925
  • enrichment culture: BTB medium (bromthymol blue)
  • enrichment culture composition: (NH4)2SO4 0.236g, sodiumcitrate 0.512g, KH2PO4 0.1g, agar 20g, 0.01g BTB, distilled water added to 1l final volume, adjusted pH to 7.0–7.3
  • enrichment culture duration: 2 days
  • enrichment culture temperature: 35

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Environmental#Aquatic#River (Creek)

Sequence information

16S sequences

  • @ref: 44076
  • description: 16S rRNA gene sequence
  • accession: KX066866
  • database: nuccore

GC content

  • @ref: 44076
  • GC-content: 64.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 44076

culture collection no.: BCRC 81061, CICC 24113

literature

  • topic: Phylogeny
  • Pubmed-ID: 29034859
  • title: Massilia neuiana sp. nov., isolated from wet soil.
  • authors: Zhao X, Li X, Qi N, Gan M, Pan Y, Han T, Hu X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002333
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44076Xin Zhao, Xuejie Li, Nan Qi, Meijun Gan, Yujin Pan, Tianfang Han and Xiaomin HuMassilia neuiana sp. nov., isolated from wet soil10.1099/ijsem.0.002333IJSEM 67: 4943-4947 201729034859