Strain identifier

BacDive ID: 14109

Type strain: Yes

Species: Pedobacter steynii

Strain Designation: WB2.3-45

Strain history: <- S. Muurholm <- E. Brambilla <- O. Päuker; WB2.3-45

NCBI tax ID(s): 430522 (species)

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@ref: 7897

BacDive-ID: 14109

DSM-Number: 19110

keywords: motile, Gram-negative, mesophilic, Bacteria, 16S sequence, genome sequence, rod-shaped

description: Pedobacter steynii WB2.3-45 is a mesophilic, Gram-negative, motile bacterium that was isolated from stream water.

NCBI tax id

  • NCBI tax id: 430522
  • Matching level: species

strain history: <- S. Muurholm <- E. Brambilla <- O. Päuker; WB2.3-45

doi: 10.13145/bacdive14109.20220920.7

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Pedobacter
  • species: Pedobacter steynii
  • full scientific name: Pedobacter steynii Muurholm et al. 2007

@ref: 7897

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Pedobacter

species: Pedobacter steynii

full scientific name: Pedobacter steynii Muurholm et al. 2007

strain designation: WB2.3-45

type strain: yes


cell morphology

  • @ref: 32230
  • gram stain: negative
  • cell length: 2.73 µm
  • cell width: 0.83 µm
  • cell shape: rod-shaped
  • motility: yes


  • @ref: 32230
  • production: no

Culture and growth conditions

culture medium

7897R2A MEDIUM (DSMZ Medium 830)yes R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l Na-pyruvate 0.3 g/l K2HPO4 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
37317MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)

culture temp


culture pH


Physiology and metabolism

spore formation

  • @ref: 32230
  • spore formation: no


@refsaltgrowthtested relationconcentration
32230NaClpositivegrowth0-4 %
32230NaClpositiveoptimum2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3223040585alpha-cyclodextrin+carbon source
3223035391aspartate+carbon source
3223017057cellobiose+carbon source
3223023652dextrin+carbon source
3223028260galactose+carbon source
322305291gelatin+carbon source
3223017234glucose+carbon source
3223029987glutamate+carbon source
3223017754glycerol+carbon source
3223028087glycogen+carbon source
3223017716lactose+carbon source
3223017306maltose+carbon source
3223037684mannose+carbon source
3223028053melibiose+carbon source
3223037657methyl D-glucoside+carbon source
32230506227N-acetylglucosamine+carbon source
3223016634raffinose+carbon source
3223017814salicin+carbon source
3223017822serine+carbon source
3223026986threonine+carbon source
3223027082trehalose+carbon source
3223053426tween 80+carbon source
3223018222xylose+carbon source


32230alkaline phosphatase+
32230cytochrome oxidase+

Isolation, sampling and environmental information


  • @ref: 7897
  • sample type: stream water
  • geographic location: Lower Saxony, Westhöfer Bach
  • country: Germany
  • DEU
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #River (Creek)

Safety information

risk assessment

  • @ref: 7897
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 32230
  • description: Pedobacter steynii partial 16S rRNA gene, type strain WB2.3-45T
  • accession: AM491372
  • length: 1505
  • database: nuccore
  • NCBI tax ID: 430522

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pedobacter steynii DSM 19110GCA_900103665scaffoldncbi430522
66792Pedobacter steynii strain DSM 19110430522.10wgspatric430522
66792Pedobacter steynii DSM 191102675903693draftimg430522

External links

@ref: 7897

culture collection no.: DSM 19110

straininfo link

  • @ref: 20218
  • passport:


Phylogeny17911287Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp. nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet.Muurholm S, Cousin S, Pauker O, Brambilla E, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.65166-02007Animals, Bacterial Typing Techniques, Bacteroidetes/chemistry/*classification/*isolation & purification, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Germany, Molecular Sequence Data, Organic Chemicals/metabolism, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidGenetics
Phylogeny24659428Pedobacter seoulensis sp. nov., isolated from soil of a bamboo field.Ngo HT, Son HM, Park SY, Kim KY, Yi THAntonie Van Leeuwenhoek10.1007/s10482-014-0154-z2014Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Pedobacter/*classification/genetics/*isolation & purification/physiology, Phospholipids/analysis, Phylogeny, Polyamines/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny33502299Pedobacter gandavensis sp. nov., Pedobacter foliorum sp. nov. and Pedobacter planticolens sp. nov., isolated from leaves of Arabidopsis thaliana.Qi SS, Cnockaert M, Carlier A, Vandamme PInt J Syst Evol Microbiol10.1099/ijsem.0.0046672021


7897Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19110)
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32230Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128472
37317Curators of the CIP
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880