Strain identifier

BacDive ID: 141081

Type strain: Yes

Species: Psychroflexus aestuariivivens

Strain Designation: DB-3

NCBI tax ID(s): 1795040 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 43978

BacDive-ID: 141081

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Psychroflexus aestuariivivens DB-3 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from tidal flat at Daebu Island, Yellow Sea in South Korea.

NCBI tax id

  • NCBI tax id: 1795040
  • Matching level: species

doi: 10.13145/bacdive141081.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Psychroflexus
  • species: Psychroflexus aestuariivivens
  • full scientific name: Psychroflexus aestuariivivens Park et al. 2016

@ref: 43978

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Psychroflexus

species: Psychroflexus aestuariivivens

strain designation: DB-3

type strain: yes

Morphology

cell morphology

  • @ref: 43978
  • gram stain: negative
  • cell length: 0.3-10.0 µm
  • cell width: 0.2-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43978
  • colony size: 0.5-1.0 mm
  • colony color: strong orange
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Marine agar 2216

pigmentation

  • @ref: 43978
  • production: no
  • name: Flexirubin-type pigments

Culture and growth conditions

culture medium

  • @ref: 43978
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43978positivegrowth4-37
43978positiveoptimum30mesophilic
43978nogrowth40thermophilic

culture pH

@refabilitytypepH
43978positiveoptimum7.0-8.0
43978positivegrowth6
43978nogrowth5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43978
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
43978NaClpositiveoptimum2-3 %(w/v)
43978NaClpositivegrowth0.5-15 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4397817057cellobiose-builds acid from
4397815824D-fructose-builds acid from
4397812936D-galactose-builds acid from
4397816899D-mannitol-builds acid from
4397816024D-mannose-builds acid from
439786731melezitose-builds acid from
4397816634raffinose-builds acid from
4397816988D-ribose-builds acid from
4397817924D-sorbitol-builds acid from
4397865327D-xylose-builds acid from
4397830849L-arabinose-builds acid from
4397862345L-rhamnose-builds acid from
4397817716lactose-builds acid from
4397828053melibiose-builds acid from
4397817268myo-inositol-builds acid from
4397817992sucrose-builds acid from
4397827082trehalose-builds acid from
4397817368hypoxanthine-hydrolysis
4397816199urea-hydrolysis
4397815318xanthine-hydrolysis
4397817632nitrate-reduction
4397818420magnesium(2+)-required for growth
4397817634D-glucose+builds acid from
4397817306maltose+builds acid from
43978casein+hydrolysis
439784853esculin+hydrolysis
439785291gelatin+hydrolysis
4397817895L-tyrosine+hydrolysis
4397828017starch+hydrolysis
4397853424tween 20+hydrolysis
4397853423tween 40+hydrolysis
4397853425tween 60+hydrolysis
4397853426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4397828971ampicillinyesyes10 µg (disc)
439783393carbenicillinyesyes100 µg (disc)
439783542cephalothinyesyes30 µg (disc)
4397817698chloramphenicolyesyes100 µg (disc)
439786472lincomycinyesyes15 µg (disc)
4397816869oleandomycinyesyes15 µg (disc)
4397818208penicillin gyesyes20 Unit (disc)
4397827902tetracyclineyesyes30 µg (disc)
4397817833gentamicinyesyes30 µg (disc)
439786104kanamycinyesyes30 µg (disc)
439787507neomycinyesyes30 µg (disc)
4397828368novobiocinyesyes5 µg (disc)
439788309polymyxin byesyes100 Unit (disc)
4397817076streptomycinyesyes50 µg (disc)

metabolite production

  • @ref: 43978
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
43978catalase+1.11.1.6
43978cytochrome oxidase+1.9.3.1
43978alkaline phosphatase+3.1.3.1
43978esterase (C 4)+
43978esterase Lipase (C 8)+
43978leucine arylamidase+3.4.11.1
43978valine arylamidase+
43978acid phosphatase+3.1.3.2
43978naphthol-AS-BI-phosphohydrolase+
43978lipase (C 14)-
43978cystine arylamidase-3.4.11.3
43978trypsin-3.4.21.4
43978alpha-chymotrypsin-3.4.21.1
43978alpha-galactosidase-3.2.1.22
43978beta-galactosidase-3.2.1.23
43978beta-glucuronidase-3.2.1.31
43978alpha-glucosidase-3.2.1.20
43978beta-glucosidase-3.2.1.21
43978N-acetyl-beta-glucosaminidase-3.2.1.52
43978alpha-mannosidase-3.2.1.24
43978alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43978C15:0 anteiso20.8
    43978C15:1 anteiso A6.9
    43978C15:0 2OH2.5
    43978C16:1ω7c / C16:1ω6c1.3
    43978C17:0 2OH5.2
    43978C15:0 iso29.9
    43978C15:0 iso 3OH2.8
    43978C15:1 iso G10.9
    43978C16:0 iso1.1
    43978C16:0 iso 3OH3.4
    43978isoC 17:0 3-OH12.3
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine agar 2216
  • agar/liquid: agar
  • incubation temperature: 30
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890 gas chromatograph
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

  • @ref: 43978
  • sample type: tidal flat at Daebu Island, Yellow Sea in South Korea
  • geographic location: Daebu Island, Yellow Sea
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • enrichment culture: Marine agar 2216
  • enrichment culture temperature: 25

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Tidal flat

taxonmaps

  • @ref: 69479
  • File name: preview.99_50377.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_423;96_1937;97_28422;98_36213;99_50377&stattab=map
  • Last taxonomy: Psychroflexus aestuariivivens subclade
  • 16S sequence: KU167714
  • Sequence Identity:
  • Total samples: 27
  • soil counts: 1
  • aquatic counts: 16
  • animal counts: 9
  • plant counts: 1

Sequence information

16S sequences

  • @ref: 43978
  • description: 16S rRNA gene sequence
  • accession: KU167714
  • database: nuccore

GC content

  • @ref: 43978
  • GC-content: 34.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43978

culture collection no.: KCTC 52037, NBRC 111757

literature

  • topic: Phylogeny
  • Pubmed-ID: 26944341
  • title: Psychroflexus aestuariivivens sp. nov., isolated from a tidal flat.
  • authors: Park S, Jung YT, Park JM, Kim SG, Yoon JH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001000
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43978Sooyeon Park, Yong-Taek Jung, Ji-Min Park, Song-Gun Kim, Jung-Hoon YoonPsychroflexus aestuariivivens sp. nov., isolated from a tidal flat10.1099/ijsem.0.001000IJSEM 66: 2146-2151 201626944341
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/