Strain identifier

BacDive ID: 141067

Type strain: Yes

Species: Halostella salina

Strain Designation: CBA1114

Strain history: S. W. Roh; Korea Basic Sci. Inst., South Korea; CBA1114.

NCBI tax ID(s): 1547897 (species)

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General

@ref: 43953

BacDive-ID: 141067

keywords: genome sequence, 16S sequence, Archaea, chemoorganotroph, Gram-negative, coccus-shaped

description: Halostella salina CBA1114 is a chemoorganotroph, Gram-negative, coccus-shaped archaeon that was isolated from solar saltern, Republic of Korea.

NCBI tax id

  • NCBI tax id: 1547897
  • Matching level: species

strain history

  • @ref: 67770
  • history: S. W. Roh; Korea Basic Sci. Inst., South Korea; CBA1114.

doi: 10.13145/bacdive141067.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Halobacteriales
  • family: Halobacteriaceae
  • genus: Halostella
  • species: Halostella salina
  • full scientific name: Halostella salina Song et al. 2016

@ref: 43953

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Halobacteriaceae

genus: Halostella

species: Halostella salina

strain designation: CBA1114

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapeconfidence
43953negative0.7-1.4 µm0.7-1.4 µmcoccus-shaped
69480negative99.979

colony morphology

  • @ref: 43953
  • colony color: red
  • medium used: DSM medium no. 954

Culture and growth conditions

culture medium

  • @ref: 43953
  • name: DSM medium no. 954
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43953positivegrowth20-50
43953positiveoptimum40thermophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
43953positivegrowth7.0-9.0alkaliphile
43953positiveoptimum8

Physiology and metabolism

nutrition type

  • @ref: 43953
  • type: chemoorganotroph

spore formation

@refspore formationconfidence
69481no96
69480no99.942

halophily

@refsaltgrowthtested relationconcentration
43953NaClpositivegrowth15-30 %(w/v)
43953NaClpositiveoptimum20 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4395328262dimethyl sulfoxide-anaerobic growth
4395316467L-arginine-anaerobic growth
4395317632nitrate-anaerobic growth
4395316947citrate-carbon source
4395315824D-fructose-carbon source
4395316988D-ribose-carbon source
4395317317D-sorbose-carbon source
4395365327D-xylose-carbon source
4395329806fumarate-carbon source
43953L-alanine 4-nitroanilide-carbon source
4395316467L-arginine-carbon source
4395329991L-aspartate-carbon source
4395318019L-lysine-carbon source
4395315729L-ornithine-carbon source
4395330911sorbitol-carbon source
43953casein-hydrolysis
439535291gelatin-hydrolysis
4395328017starch-hydrolysis
4395353426tween 80-hydrolysis
4395316301nitrite-reduction
4395315824D-fructose+builds acid from
4395312936D-galactose+builds acid from
4395317634D-glucose+builds acid from
4395316024D-mannose+builds acid from
4395317317D-sorbose+builds acid from
4395312936D-galactose+carbon source
4395317634D-glucose+carbon source
4395316024D-mannose+carbon source
4395324996lactate+carbon source
4395317754glycerol+carbon source
4395316411indole-3-acetic acid+carbon source
4395329985L-glutamate+carbon source
43953maltose hydrate+carbon source
4395315361pyruvate+carbon source
4395328017starch+carbon source
4395317992sucrose+carbon source
4395353423tween 40+hydrolysis
4395317632nitrate+reduction
4395318420magnesium(2+)+required for growth
4395315428glycine+/-carbon source
4395315589L-malate+/-carbon source
4395317716lactose+/-carbon source
4395329864mannitol+/-carbon source
4395330031succinate+/-carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
43953338412(-)-anisomycinyesyes20 µg (disc)
4395328669bacitracinyesyes0.1 Unit (disc)
4395317698chloramphenicolyesyes50 µg (disc)
43953100241ciprofloxacinyesyes10 µg (disc)
4395348923erythromycinyesyes25 µg (disc)
439537507neomycinyesyes50 µg (disc)
43953100246norfloxacinyesyes20 µg (disc)
4395328368novobiocinyesyes50 µg (disc)
4395328077rifampicinyesyes10 µg (disc)
4395328971ampicillinyesyes20 µg (disc)
4395318208penicillin gyesyes20 Unit (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4395316136hydrogen sulfideno
4395335581indoleno

enzymes

@refvalueactivityec
43953catalase+1.11.1.6
43953cytochrome oxidase-1.9.3.1
43953urease-3.5.1.5
43953phenylalanine deaminase-4.3.1.5
43953lysine decarboxylase-4.1.1.18
43953ornithine decarboxylase-4.1.1.17
43953arginine dihydrolase-3.5.3.6

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
43953solar saltern, Republic of KoreaRepublic of KoreaKORAsia34.6092126.287DSM medium no. 9545 g/L yeast extract, 5 g/L Casamino acids, 20 g/L MgCl2.6H2O, 2 g/L KCl, 12 g/L Tris, 0.2 g/L CaCl2.2H2O, 200 g/L NaCl and 20 g/L agar, pH 7.48 weeks37
67770Solar salternRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2
#Condition#Saline
#Environmental#Terrestrial

taxonmaps

  • @ref: 69479
  • File name: preview.99_189578.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18293;96_69977;97_90881;98_123496;99_189578&stattab=map
  • Last taxonomy: Halostella salina subclade
  • 16S sequence: KR053261
  • Sequence Identity:
  • Total samples: 116
  • soil counts: 41
  • aquatic counts: 56
  • animal counts: 5
  • plant counts: 14

Sequence information

16S sequences

@refdescriptionaccessiondatabase
4395316S rRNA rrnA gene sequenceKM068159nuccore
4395316S rRNA rrnB gene sequenceKR053260nuccore
4395316S rRNA rrnC gene sequenceKR053261nuccore

Genome sequences

  • @ref: 67770
  • description: Halostella salina CBA1114
  • accession: GCA_003675855
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1547897

GC content

@refGC-contentmethod
4395368.1sequence analysis
6777068.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
motileno70.513no
flagellatedno96.198no
gram-positiveno96.573yes
anaerobicno95.235no
aerobicyes85.009no
halophileyes86.245yes
spore-formingno94.613no
glucose-utilyes91.317yes
thermophileno71.522no
glucose-fermentno82.329no

External links

@ref: 43953

culture collection no.: JCM 30111, KCTC 4206

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27118259Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt.Song HS, Cha IT, Rhee JK, Yim KJ, Kim AY, Choi JS, Baek SJ, Seo MJ, Park SJ, Nam YD, Roh SWInt J Syst Evol Microbiol10.1099/ijsem.0.0011182016Base Composition, DNA, Archaeal/genetics, Genes, Archaeal, Halobacteriaceae/*classification/genetics/isolation & purification, Multilocus Sequence Typing, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Sodium ChlorideTranscriptome
Phylogeny31395107Halostella limicola sp. nov., isolated from saline soil sampled at the Tarim Basin.Han D, Hong LG, Xu Q, Cui HLInt J Syst Evol Microbiol10.1099/ijsem.0.0036432019Bacterial Typing Techniques, Base Composition, China, DNA, Archaeal/genetics, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Glycolipids/chemistry, Halobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome
Phylogeny31971500Halostella pelagica sp. nov. and Halostella litorea sp. nov., isolated from salted brown alga Laminaria.Han D, Cui HLInt J Syst Evol Microbiol10.1099/ijsem.0.0040032020Base Composition, China, DNA, Archaeal/genetics, Genes, Archaeal, Glycolipids/chemistry, Halobacteriaceae/*classification/isolation & purification, Laminaria/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43953Hye Seon Song, In-Tae Cha, Jin-Kyu Rhee, Kyung June Yim, Ah Yoon Kim, Jong-Soon Choi, Su Jeong Baek, Myung-Ji Seo, Soo-Je Park, Young-Do Nam, Seong Woon RohHalostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt10.1099/ijsem.0.001118IJSEM 66: 2740-2746 201627118259
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1