Strain identifier
BacDive ID: 141050
Type strain: 
Species: Salinifilum proteinilyticum
Strain Designation: Miq-12
NCBI tax ID(s): 1851175 (species)
version 8.1 (current version)
General
@ref: 43930
BacDive-ID: 141050
keywords: 16S sequence, Bacteria, aerobe, halophilic, Gram-positive, colony-forming
description: Salinifilum proteinilyticum Miq-12 is an aerobe, halophilic, Gram-positive bacterium that forms irregular colonies and was isolated from Meighan wetland, Iran.
NCBI tax id
- NCBI tax id: 1851175
- Matching level: species
doi: 10.13145/bacdive141050.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Salinifilum
- species: Salinifilum proteinilyticum
- full scientific name: Salinifilum proteinilyticum Moshtaghi Nikou et al. 2017
@ref: 43930
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Pseudonocardiales
family: Pseudonocardiaceae
genus: Salinifilum
species: Salinifilum proteinilyticum
strain designation: Miq-12
type strain: yes
Morphology
cell morphology
- @ref: 43930
- gram stain: positive
colony morphology
- @ref: 43930
- colony color: colourless - pale green
- colony shape: irregular
- medium used: ISP 2 agar
pigmentation
- @ref: 43930
- production: no
- name: diffusible pigments
Culture and growth conditions
culture medium
- @ref: 43930
- name: ISP 2 agar
- growth: yes
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 43930 | positive | growth | 30-50 | |
| 43930 | positive | optimum | 40 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 43930 | positive | growth | 5.5-10.5 | alkaliphile |
| 43930 | positive | optimum | 7.0-8.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 43930
- oxygen tolerance: aerobe
halophily
| @ref | halophily level | salt | growth | tested relation | concentration |
|---|---|---|---|---|---|
| 43930 | halophilic | NaCl | positive | growth | 12-25 % |
| 43930 | NaCl | positive | optimum | 15-20 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43930 | 17057 | cellobiose | - | carbon source |
| 43930 | 17108 | D-arabinose | - | carbon source |
| 43930 | 12936 | D-galactose | - | carbon source |
| 43930 | 17716 | lactose | - | carbon source |
| 43930 | 17306 | maltose | - | carbon source |
| 43930 | 29864 | mannitol | - | carbon source |
| 43930 | 28053 | melibiose | - | carbon source |
| 43930 | 16708 | adenine | - | hydrolysis |
| 43930 | 16235 | guanine | - | hydrolysis |
| 43930 | 28017 | starch | - | hydrolysis |
| 43930 | 53424 | tween 20 | - | hydrolysis |
| 43930 | 53425 | tween 60 | - | hydrolysis |
| 43930 | 15318 | xanthine | - | hydrolysis |
| 43930 | 17632 | nitrate | - | reduction |
| 43930 | 15824 | D-fructose | + | carbon source |
| 43930 | 16024 | D-mannose | + | carbon source |
| 43930 | 16988 | D-ribose | + | carbon source |
| 43930 | 65327 | D-xylose | + | carbon source |
| 43930 | 17268 | myo-inositol | + | carbon source |
| 43930 | 26546 | rhamnose | + | carbon source |
| 43930 | 17814 | salicin | + | carbon source |
| 43930 | 27082 | trehalose | + | carbon source |
| 43930 | casein | + | hydrolysis | |
| 43930 | 17634 | D-glucose | + | hydrolysis |
| 43930 | 17368 | hypoxanthine | + | hydrolysis |
| 43930 | 53423 | tween 40 | + | hydrolysis |
| 43930 | 53426 | tween 80 | + | hydrolysis |
| 43930 | 18186 | tyrosine | + | hydrolysis |
metabolite production
- @ref: 43930
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
Isolation, sampling and environmental information
isolation
- @ref: 43930
- sample type: Meighan wetland, Iran
- geographic location: Meighan wetland
- country: Iran
- origin.country: IRN
- continent: Asia
- enrichment culture: modified growth medium
- enrichment culture composition: NaCl 184 g/L; MgCl2.6H2O 23 g/L; MgSO4 26.83 g/L; KCl 7.76 g/L; NaBr 0.61 g/L; peptone 5.0 g/L; yeast extract 1.0 g/L, agar 18 g/L, pH 7.5
- enrichment culture duration: 3 weeks
- enrichment culture temperature: 40
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Wetland (Swamp)
taxonmaps
- @ref: 69479
- File name: preview.99_86985.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6153;96_27325;97_34356;98_60160;99_86985&stattab=map
- Last taxonomy: Salinifilum proteinilyticum subclade
- 16S sequence: KX024578
- Sequence Identity:
- Total samples: 453
- soil counts: 112
- aquatic counts: 61
- animal counts: 251
- plant counts: 29
Sequence information
16S sequences
- @ref: 43930
- description: 16S rRNA gene sequence
- accession: KX024578
- database: nuccore
GC content
- @ref: 43930
- GC-content: 72.1
- method: high performance liquid chromatography (HPLC)
External links
@ref: 43930
culture collection no.: IBRCM 11033, LMG 28390
literature
- topic: Phylogeny
- Pubmed-ID: 28920832
- title: Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov.
- authors: Moshtaghi Nikou M, Ramezani M, Harirchi S, Makzoom S, Amoozegar MA, Shahzadeh Fazeli SA, Schumann P, Ventosa A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002286
- year: 2017
- mesh: Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Halobacteriales/classification, Iran, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/*classification, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Wetlands
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 43930 | Mahdi Moshtaghi Nikou, Mohaddaseh Ramezani, Sharareh Harirchi, Somayyeh Makzoom, Mohammad Ali Amoozegar, Seyed Abolhassan Shahzadeh Fazeli, Peter Schumann, Antonio Ventosa | Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov | 10.1099/ijsem.0.002286 | IJSEM 67: 4221-4227 2017 | 28920832 |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |