Strain identifier

BacDive ID: 141001

Type strain: Yes

Species: Spiribacter roseus

Strain Designation: SP30

NCBI tax ID(s): 1935996 (species)

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General

@ref: 43888

BacDive-ID: 141001

keywords: 16S sequence, Bacteria, aerobe, moderately halophilic, Gram-negative, rod-shaped, colony-forming

description: Spiribacter roseus SP30 is an aerobe, moderately halophilic, Gram-negative bacterium that forms circular colonies and was isolated from water sample with 34.2 % total salts of pond of a marine solar saltern, Santa Pola, Alicante, East coast of Spain.

NCBI tax id

  • NCBI tax id: 1935996
  • Matching level: species

doi: 10.13145/bacdive141001.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Ectothiorhodospiraceae
  • genus: Spiribacter
  • species: Spiribacter roseus
  • full scientific name: Spiribacter roseus León et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Spiribacter aquaticus

@ref: 43888

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Ectothiorhodospiraceae

genus: Spiribacter

species: Spiribacter aquaticus

strain designation: SP30

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43888negative1.5-1.8 µm0.4 µmrod-shapedno
43888curved-shaped
43888spiral-shaped
43888ring-shaped

colony morphology

  • @ref: 43888
  • colony size: 0.5-1.0 mm
  • colony color: yellow-brown
  • colony shape: circular
  • incubation period: 5 days
  • medium used: SMM medium

Culture and growth conditions

culture medium

  • @ref: 43888
  • name: SMM medium
  • growth: yes
  • composition: NaCl 46.8 g/L, MgCl 2.6H2O 19.5 g/L, MgSO4.7H2O 30.5 g/L, CaCl2 0.5 g/L, KCl3.0 g/L, NaHCO3 0.1 g/L, NaBr 0.35 g/L, casein digest 5.0 g/L, sodium pyruvate 1.1 g/L, pH 7.5

culture temp

@refgrowthtypetemperaturerange
43888positivegrowth20-40
43888positiveoptimum37mesophilic

culture pH

@refabilitytypepHPH range
43888positivegrowth7.0-9.0alkaliphile
43888positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 43888
  • oxygen tolerance: aerobe

halophily

@refhalophily levelsaltgrowthtested relationconcentration
43888moderately halophilicNaClpositivegrowth7.5-25 %(w/v)
43888NaClpositiveoptimum12.5 %(w/v)
43888NaClpositivegrowth0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4388817108D-arabinose-builds acid from
4388815824D-fructose-builds acid from
4388812936D-galactose-builds acid from
4388817634D-glucose-builds acid from
4388816024D-mannose-builds acid from
4388865327D-xylose-builds acid from
4388817306maltose-builds acid from
438886731melezitose-builds acid from
4388816634raffinose-builds acid from
4388827082trehalose-builds acid from
4388816150benzoate-carbon source
4388817057cellobiose-carbon source
4388816947citrate-carbon source
4388817108D-arabinose-carbon source
4388812936D-galactose-carbon source
4388817634D-glucose-carbon source
4388816899D-mannitol-carbon source
4388816024D-mannose-carbon source
4388817924D-sorbitol-carbon source
4388830927D-tartrate-carbon source
4388815740formate-carbon source
4388828757fructose-carbon source
4388828300glutamine-carbon source
43888606565hippurate-carbon source
43888L-alanine 4-nitroanilide-carbon source
4388816467L-arginine-carbon source
4388817196L-asparagine-carbon source
4388817561L-cysteine-carbon source
4388817191L-isoleucine-carbon source
4388818019L-lysine-carbon source
4388816643L-methionine-carbon source
4388815729L-ornithine-carbon source
4388817295L-phenylalanine-carbon source
4388817115L-serine-carbon source
4388830924L-tartrate-carbon source
4388816857L-threonine-carbon source
4388816414L-valine-carbon source
4388817716lactose-carbon source
4388817306maltose-carbon source
438886731melezitose-carbon source
4388817790methanol-carbon source
4388816634raffinose-carbon source
4388830031succinate-carbon source
4388827082trehalose-carbon source
4388817151xylitol-carbon source
4388828300glutamine-energy source
43888L-alanine 4-nitroanilide-energy source
4388816467L-arginine-energy source
4388817196L-asparagine-energy source
4388817561L-cysteine-energy source
4388817191L-isoleucine-energy source
4388818019L-lysine-energy source
4388816643L-methionine-energy source
4388815729L-ornithine-energy source
4388817295L-phenylalanine-energy source
4388817115L-serine-energy source
4388816857L-threonine-energy source
4388816414L-valine-energy source
4388817234glucose-fermentation
4388816991dna-hydrolysis
438884853esculin-hydrolysis
438885291gelatin-hydrolysis
4388828017starch-hydrolysis
4388853426tween 80-hydrolysis
4388828300glutamine-nitrogen source
43888L-alanine 4-nitroanilide-nitrogen source
4388816467L-arginine-nitrogen source
4388817196L-asparagine-nitrogen source
4388817561L-cysteine-nitrogen source
4388817191L-isoleucine-nitrogen source
4388818019L-lysine-nitrogen source
4388816643L-methionine-nitrogen source
4388815729L-ornithine-nitrogen source
4388817295L-phenylalanine-nitrogen source
4388817115L-serine-nitrogen source
4388816857L-threonine-nitrogen source
4388816414L-valine-nitrogen source
4388817632nitrate-reduction
4388816988D-ribose+builds acid from
4388815361pyruvate+carbon source

metabolite production

@refChebi-IDmetaboliteproduction
4388815688acetoinno
4388835581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testcitrate test
4388817234glucose-
4388815688acetoin-
4388816947citrate-

enzymes

@refvalueactivityec
43888catalase+1.11.1.6
43888cytochrome oxidase+1.9.3.1
43888phosphatase+
43888urease+3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 43888
  • sample type: water sample with 34.2 % (w/v) total salts of pond of a marine solar saltern, Santa Pola, Alicante, East coast of Spain
  • sampling date: 2015-05
  • geographic location: Santa Pola, Alicante
  • country: Spain
  • origin.country: ESP
  • continent: Europe
  • enrichment culture: Seawater salts solution (25 % w/v)
  • enrichment culture composition: NaCl 195 g/L, MgCl2.6H2O 34.6 g/L, MgSO4.7H2O 49.5 g/L, CaCl2 0.7 g/L, KCl 5.0 g/L, NaHCO3 0.17 g/L, NaBr 0.65 g/L, pH 7.5, supplemented with 0.1 % yeast extract, solidified with 2 % agar
  • enrichment culture duration: 4 weeks
  • enrichment culture temperature: 37

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Pond (small)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5259.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15363;96_653;97_3122;98_3921;99_5259&stattab=map
  • Last taxonomy: Spiribacter
  • 16S sequence: LT714150
  • Sequence Identity:
  • Total samples: 905
  • soil counts: 128
  • aquatic counts: 691
  • animal counts: 71
  • plant counts: 15

Sequence information

16S sequences

  • @ref: 43888
  • description: 16S rRNA gene sequence
  • accession: LT714150
  • database: nuccore

GC content

  • @ref: 43888
  • GC-content: 65.4
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 43888

culture collection no.: CECT 9238, LMG 30005

literature

  • topic: Phylogeny
  • Pubmed-ID: 28820120
  • title: Spiribacter aquaticus sp. nov., a novel member of the genus Spiribacter isolated from a saltern.
  • authors: Leon MJ, Aldeguer-Riquelme B, Anton J, Sanchez-Porro C, Ventosa A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002053
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Spain
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43888María José León, Borja Aldeguer-Riquelme, Josefa Antón, Cristina Sánchez-Porro, Antonio VentosaSpiribacter aquaticus sp. nov., a novel member of the genus Spiribacter isolated from a saltern10.1099/ijsem.0.002053IJSEM 67: 2947-2952 201728820120
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/