Strain identifier

BacDive ID: 140974

Type strain: Yes

Species: Blastomonas fulva

Strain Designation: T2

Strain history: <- Long Jin, KRIBB

NCBI tax ID(s): 1550728 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 67682

BacDive-ID: 140974

DSM-Number: 112298

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped

description: Blastomonas fulva T2 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from culture of Microcystis from Daejeon, Republic of Korea.

NCBI tax id

  • NCBI tax id: 1550728
  • Matching level: species

strain history

@refhistory
67682<- JCM; JCM 30467 <- L. Jin, KRIBB - Korea Res. Inst. Biosci. Biotechn.; T2
67770L. Jin; KRIBB, South Korea; T2.
67771<- Long Jin, KRIBB

doi: 10.13145/bacdive140974.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingomonadaceae
  • genus: Blastomonas
  • species: Blastomonas fulva
  • full scientific name: Blastomonas fulva Lee et al. 2017

@ref: 67682

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Blastomonas

species: Blastomonas fulva

full scientific name: Blastomonas fulva Lee et al. 2017

strain designation: T2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43866negative1.0-3.0 µm0.4-0.7 µmrod-shapedno
69480negative99.997

colony morphology

  • @ref: 43866
  • colony color: yellow
  • incubation period: 2 days
  • medium used: R2A agar

multimedia

  • @ref: 67682
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_112298.jpg
  • caption: DSM 112298 on medium 830 at 28 °C
  • intellectual property rights: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43866Reasoner's 2A agar (R2A)yes
67682R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43866positivegrowth8-37
43866positiveoptimum30mesophilic
43866nogrowth4psychrophilic
43866nogrowth45thermophilic
67682positivegrowth28mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepH
43866positivegrowth6.0-8.0
43866positiveoptimum7
43866nogrowth5
43866nogrowth9

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43866aerobe
67771aerobe

spore formation

@refspore formationconfidence
43866no
69481no100
69480no99.968

halophily

  • @ref: 43866
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43866168082-dehydro-D-gluconate-assimilation
43866161933-hydroxybenzoate-assimilation
43866178794-hydroxybenzoate-assimilation
43866581435-dehydro-D-gluconate-assimilation
4386617128adipate-assimilation
4386627689decanoate-assimilation
4386616947citrate-assimilation
4386616899D-mannitol-assimilation
4386616024D-mannose-assimilation
4386616988D-ribose-assimilation
4386617924D-sorbitol-assimilation
4386624265gluconate-assimilation
4386628087glycogen-assimilation
4386627570histidine-assimilation
4386617240itaconate-assimilation
43866L-alanine 4-nitroanilide-assimilation
4386630849L-arabinose-assimilation
4386618287L-fucose-assimilation
4386617203L-proline-assimilation
4386617115L-serine-assimilation
4386624996lactate-assimilation
4386625115malate-assimilation
4386615792malonate-assimilation
4386617306maltose-assimilation
4386628053melibiose-assimilation
4386617268myo-inositol-assimilation
43866506227N-acetylglucosamine-assimilation
4386618401phenylacetate-assimilation
4386617814salicin-assimilation
4386617234glucose-builds acid from
438664853esculin-hydrolysis
438665291gelatin-hydrolysis
4386617632nitrate-reduction
4386630089acetate+assimilation
4386617634D-glucose+assimilation
43866370543-hydroxybutyrate+assimilation
4386662345L-rhamnose+assimilation
4386617272propionate+assimilation
438669300suberic acid+assimilation
4386617992sucrose+assimilation
4386631011valerate+assimilation
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
4386635581indoleno
6836935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43866catalase+1.11.1.6
43866cytochrome oxidase+1.9.3.1
43866urease-3.5.1.5
43866acid phosphatase+3.1.3.2
43866alkaline phosphatase+3.1.3.1
43866alpha-chymotrypsin+3.4.21.1
43866esterase (C 4)+
43866esterase Lipase (C 8)+
43866beta-galactosidase+3.2.1.23
43866leucine arylamidase+3.4.11.1
43866naphthol-AS-BI-phosphohydrolase+/-
43866N-acetyl-beta-glucosaminidase-3.2.1.52
43866cystine arylamidase-3.4.11.3
43866alpha-fucosidase-3.2.1.51
43866alpha-galactosidase-3.2.1.22
43866alpha-glucosidase-3.2.1.20
43866beta-glucosidase-3.2.1.21
43866beta-glucuronidase-3.2.1.31
43866lipase (C 14)-
43866alpha-mannosidase-3.2.1.24
43866trypsin-3.4.21.4
43866valine arylamidase-
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43866C15:0 2OH3.5
    43866C16:04.9
    43866C16:1ω5c1
    43866C16:1ω7c / C16:1ω6c3.9
    43866C17:08
    43866C17:1ω6c48.9
    43866C17:1ω8c8.1
    43866C18:1ω7c / C18:1ω6c17.2
  • type of FA analysis: whole cell analysis
  • incubation medium: Tryptic soy agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890 gas chromatograph
  • cutoff value:

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
67682-------+------------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture temperaturelatitudelongitudehost species
43866culture of Microcystis from Daejeon, Republic of KoreaDaejeonRepublic of KoreaKORAsiaR2A agar25
67682Microcystis cultureDaejeonRepublic of KoreaKORAsia36.35127.417
67770Culture of Microcystis from DaejeonRepublic of KoreaKORAsiaMicrocystis
67771From a culture of Microcystis from DaejeonRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Host
  • Cat2: #Microbial
  • Cat3: #Bacteria

taxonmaps

  • @ref: 69479
  • File name: preview.99_4300.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_574;97_668;98_3237;99_4300&stattab=map
  • Last taxonomy: Blastomonas fulva
  • 16S sequence: KM670031
  • Sequence Identity:
  • Total samples: 8418
  • soil counts: 1631
  • aquatic counts: 4621
  • animal counts: 1227
  • plant counts: 939

Safety information

risk assessment

  • @ref: 67682
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43866
  • description: 16S rRNA gene sequence
  • accession: KM670031
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Blastomonas fulva T2GCA_003431825completencbi1550728
66792Blastomonas fulva strain T21550728.3completepatric1550728

GC content

  • @ref: 67770
  • GC-content: 64.2
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes60.53yes
flagellatedno88.07yes
gram-positiveno97.187no
anaerobicno99.23yes
aerobicyes95.969yes
halophileno89.441no
spore-formingno94.474yes
glucose-utilyes80.854no
thermophileno97.991no
glucose-fermentno94.336no

External links

@ref: 67682

culture collection no.: KCTC 42354, JCM 30467, DSM 112298

straininfo link

  • @ref: 96821
  • straininfo: 397091

literature

  • topic: Phylogeny
  • Pubmed-ID: 28820119
  • title: Blastomonas fulva sp. nov., aerobic photosynthetic bacteria isolated from a Microcystis culture.
  • authors: Lee HG, Ko SR, Lee JW, Lee CS, Ahn CY, Oh HM, Jin L
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002084
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Microcystis, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43866Hyung-Gwan Lee, So Ra Ko, Jun-Woo Lee, Chang Soo Lee, Chi-Yong Ahn, Hee-Mock Oh Long JinBlastomonas fulva sp. nov., aerobic photosynthetic bacteria isolated from a Microcystis culture10.1099/ijsem.0.002084IJSEM 67: 3071-3076 201728820119
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67682Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-112298Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 112298)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96821Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397091.1