Strain identifier

BacDive ID: 140950

Type strain: Yes

Species: Cryptosporangium eucalypti

Strain Designation: EURKPP3H10

NCBI tax ID(s): 1717726 (species)

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General

@ref: 43847

BacDive-ID: 140950

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, colony-forming, pigmented

description: Cryptosporangium eucalypti EURKPP3H10 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and has a Reddish brown pigmentation.

NCBI tax id

  • NCBI tax id: 1717726
  • Matching level: species

doi: 10.13145/bacdive140950.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Cryptosporangiales
  • family: Cryptosporangiaceae
  • genus: Cryptosporangium
  • species: Cryptosporangium eucalypti
  • full scientific name: Cryptosporangium eucalypti Himaman et al. 2017

@ref: 43847

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Cryptosporangiales

family: Cryptosporangiaceae

genus: Cryptosporangium

species: Cryptosporangium eucalypti

strain designation: EURKPP3H10

type strain: yes

Morphology

cell morphology

  • @ref: 43847
  • gram stain: positive

colony morphology

@refcolony colorcolony shapeincubation periodmedium used
43847orange-yellowcircular21 daysISP 2 agar
43847orange-yellowcircular21 daysISP 3 agar
43847orange-yellowcircular21 daysISP 4 agar
43847orange-yellowcircular21 daysISP 5 agar
43847orange-yellowcircular21 daysISP 6 agar
43847orange-yellowcircular21 daysBenett's agar
43847orange-yellowcircular21 daysYS agar
43847orange-yellowcircular21 daysGYE agar
43847reddish-browncircular21 daysISP 7 agar

pigmentation

  • @ref: 43847
  • production: yes
  • color: Reddish brown
  • name: Reddish brown soluble pigments (on ISP 7 agar)

Culture and growth conditions

culture medium

@refnamegrowthcomposition
43847Bennett's agaryes
43847GYE agaryes1.0% (w/v) glucose, 1.0% (w/v) yeast extract and 1.5% (w/v) agar
43847ISP 2 agaryes
43847ISP 3 agaryes
43847ISP 4 agaryes
43847ISP 5 agaryes
43847ISP 6 agaryes
43847ISP 7 agaryes
43847Soil extract agaryes
43847Yeast extract-soluble starch agaryes

culture temp

@refgrowthtypetemperaturerange
43847positivegrowth20-40
43847positiveoptimum28-37mesophilic
43847nogrowth45thermophilic

culture pH

  • @ref: 43847
  • ability: positive
  • type: growth
  • pH: 6.0-9.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43847
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43847
  • spore formation: yes

halophily

  • @ref: 43847
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4384753258sodium citrate-carbon source
4384716708adenine-degradation
4384718305arbutin-degradation
4384762968cellulose-degradation
438475291gelatin-degradation
4384716235guanine-degradation
4384753426tween 80-degradation
4384715318xanthine-degradation
4384737166xylan-degradation
4384748095(-)-D-fructose+builds acid from
4384716899D-mannitol+builds acid from
4384765327D-xylose+builds acid from
4384717057cellobiose+builds acid from
4384717634D-glucose+builds acid from
4384716988D-ribose+builds acid from
4384727922sorbose+builds acid from
4384748095(-)-D-fructose+carbon source
4384716899D-mannitol+carbon source
4384716024D-mannose+carbon source
4384765327D-xylose+carbon source
4384717634D-glucose+carbon source
4384717306maltose+carbon source
4384728053melibiose+carbon source
43847casein+degradation
438474853esculin+degradation
4384717368hypoxanthine+degradation
4384728017starch+degradation
4384718186tyrosine+degradation
4384717151xylitol+/-builds acid from
4384762345L-rhamnose+/-carbon source
4384736218beta-lactose+/-carbon source
4384727922sorbose+/-carbon source
4384717992sucrose+/-carbon source
4384717151xylitol+/-carbon source

enzymes

@refvalueactivityec
43847catalase+1.11.1.6
43847cytochrome oxidase-1.9.3.1
43847urease-3.5.1.5
43847alkaline phosphatase+3.1.3.1
43847trypsin+3.4.21.4
43847acid phosphatase+3.1.3.2
43847esterase (C 4)+/-
43847esterase Lipase (C 8)
43847leucine aminopeptidase+/-3.4.1.1
43847valine aminopeptidase+/-
43847cystine aminopeptidase+/-3.4.11.3
43847phosphoamidase+/-3.9.1.1
43847alpha-galactosidase+/-3.2.1.22
43847beta-galactosidase+/-3.2.1.23
43847alpha-glucosidase+/-3.2.1.20
43847beta-glucosidase+/-3.2.1.21
43847lipase (C 14)-
43847chymotrypsin-3.4.4.5
43847beta-glucuronidase-3.2.1.31
43847N-acetyl-beta-glucosaminidase-3.2.1.52
43847alpha-mannosidase-3.2.1.24
43847alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

  • @ref: 43847
  • sample type: surface-sterilized Eucalyptus camaldulensis roots, collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand
  • geographic location: Kamphaengphet province
  • country: Thailand
  • origin.country: THA
  • continent: Asia
  • enrichment culture: Humic acid-vitamin agar (HV)
  • enrichment culture composition: supplemented with nalidixic acid (25 µg/mL) and ketoconazole (100 µg/mL)
  • enrichment culture duration: 2 weeks
  • enrichment culture temperature: 28

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Root (Rhizome)
#Host Body-Site#Plant#Sterilized plant part

Sequence information

16S sequences

  • @ref: 43847
  • description: 16S rRNA gene sequence
  • accession: LC085570
  • database: nuccore

GC content

  • @ref: 43847
  • GC-content: 71.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43847

culture collection no.: BCC 77605, NBRC 111482

literature

  • topic: Phylogeny
  • Pubmed-ID: 28857736
  • title: Cryptosporangium eucalypti sp. nov., an actinomycete isolated from Eucalyptus camaldulensis roots.
  • authors: Himaman W, Suksaard P, Mingma R, Matsumoto A, Duangmal K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002086
  • year: 2017
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Eucalyptus/*microbiology, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43847Winanda Himaman, Paweena Suksaard, Ratchanee Mingma, Atsuko Matsumoto, Kannika DuangmalCryptosporangium eucalypti sp. nov., an actinomycete isolated from Eucalyptus camaldulensis roots10.1099/ijsem.0.002086IJSEM 67: 3077-3082 201728857736