Strain identifier

BacDive ID: 140873

Type strain: Yes

Species: Ampullimonas aquatilis

Strain Designation: B15.09-116

NCBI tax ID(s): 1341549 (species)

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General

@ref: 43699

BacDive-ID: 140873

keywords: 16S sequence, Bacteria, obligate aerobe, organotroph, Gram-negative, motile, rod-shaped

description: Ampullimonas aquatilis B15.09-116 is an obligate aerobe, organotroph, Gram-negative bacterium that was isolated from bottled mineral water.

NCBI tax id

  • NCBI tax id: 1341549
  • Matching level: species

doi: 10.13145/bacdive140873.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Ampullimonas
  • species: Ampullimonas aquatilis
  • full scientific name: Ampullimonas aquatilis França et al. 2016

@ref: 43699

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Ampullimonas

species: Ampullimonas aquatilis

strain designation: B15.09-116

type strain: yes

Morphology

cell morphology

  • @ref: 43699
  • gram stain: negative
  • cell length: 1-2 µm
  • cell width: 1 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43699
  • colony color: non-pigmented
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 43699
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43699positivegrowth15-35
43699positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
43699positivegrowth5.5-9alkaliphile
43699positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43699
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 43699
  • type: organotroph

spore formation

  • @ref: 43699
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43699NaClpositivegrowth0-0.5 %(w/v)
43699NaClpositiveoptimum0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43699309162-oxoglutarate-assimilation
4369935391aspartate-assimilation
4369917057cellobiose-assimilation
4369916947citrate-assimilation
4369918333D-arabitol-assimilation
4369915824D-fructose-assimilation
4369912936D-galactose-assimilation
4369918391D-gluconate-assimilation
4369917315D-glucosamine-assimilation
4369917634D-glucose-assimilation
4369916899D-mannitol-assimilation
4369916024D-mannose-assimilation
4369923652dextrin-assimilation
4369917113erythritol-assimilation
4369915740formate-assimilation
4369916977L-alanine-assimilation
4369930849L-arabinose-assimilation
4369917196L-asparagine-assimilation
4369918287L-fucose-assimilation
4369929985L-glutamate-assimilation
4369915971L-histidine-assimilation
4369915603L-leucine-assimilation
4369915729L-ornithine-assimilation
4369917295L-phenylalanine-assimilation
4369917203L-proline-assimilation
4369962345L-rhamnose-assimilation
4369917115L-serine-assimilation
4369917716lactose-assimilation
4369917306maltose-assimilation
4369928053melibiose-assimilation
4369917268myo-inositol-assimilation
43699506227N-acetylglucosamine-assimilation
4369916634raffinose-assimilation
4369915963ribitol-assimilation
4369930911sorbitol-assimilation
4369917992sucrose-assimilation
4369927082trehalose-assimilation
4369917151xylitol-assimilation
43699casein-hydrolysis
436994853esculin-hydrolysis
436995291gelatin-hydrolysis
4369928017starch-hydrolysis
4369953424tween 20-hydrolysis
4369953423tween 40-hydrolysis
4369916383cis-aconitate-respiration
4369916947citrate-respiration
4369915824D-fructose-respiration
436998391D-gluconate-respiration
4369917634D-glucose-respiration
4369916899D-mannitol-respiration
4369933801D-saccharate-respiration
4369917924D-sorbitol-respiration
4369916000ethanolamine-respiration
4369915740formate-respiration
4369917754glycerol-respiration
4369929985L-glutamate-respiration
4369917203L-proline-respiration
4369915792malonate-respiration
4369917272propionate-respiration
4369926490quinate-respiration
43699143136succinamate-respiration
4369930031succinate-respiration
4369917992sucrose-respiration
4369953423tween 40-respiration
4369924996lactate+assimilation
4369925115malate+assimilation
4369917754glycerol+assimilation
4369915361pyruvate+assimilation
4369930031succinate+assimilation
4369953425tween 60+hydrolysis
4369953426tween 80+hydrolysis
4369917632nitrate+reduction
43699yeast extract+required for growth
436998295beta-hydroxybutyrate+respiration
4369924996lactate+respiration
4369951850methyl pyruvate+respiration
4369953426tween 80+respiration

enzymes

@refvalueactivityec
43699catalase-1.11.1.6
43699alkaline phosphatase-3.1.3.1
43699esterase (C 4)-
43699esterase Lipase (C 8)-
43699valine arylamidase-
43699acid phosphatase-3.1.3.2
43699naphthol-AS-BI-phosphohydrolase-
43699beta-glucosidase-3.2.1.21
43699N-acetyl-beta-glucosaminidase-3.2.1.52
43699DNase-
43699lipase (C 14)-
43699cystine arylamidase-3.4.11.3
43699trypsin-3.4.21.4
43699alpha-chymotrypsin-3.4.21.1
43699alpha-galactosidase-3.2.1.22
43699beta-galactosidase-3.2.1.23
43699beta-glucuronidase-3.2.1.31
43699alpha-glucosidase-3.2.1.20
43699alpha-mannosidase-3.2.1.24
43699alpha-fucosidase-3.2.1.51
43699cytochrome oxidase+1.9.3.1
43699leucine arylamidase+3.4.11.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43699C15:032.1
    43699C16:1ω5c9.4
    43699C16:1ω7c / C16:1ω6c11.7
    43699C15:0 iso6.8
    43699C17:0 iso 3OH20.5
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.0
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43699
  • sample type: bottled mineral water
  • geographic location: Portugal
  • country: Portugal
  • origin.country: PRT
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Beverage
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_14062.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_173;96_7258;97_8704;98_10671;99_14062&stattab=map
  • Last taxonomy: Ampullimonas aquatilis subclade
  • 16S sequence: JX458401
  • Sequence Identity:
  • Total samples: 7012
  • soil counts: 882
  • aquatic counts: 3064
  • animal counts: 2641
  • plant counts: 425

Sequence information

16S sequences

  • @ref: 43699
  • description: 16S rRNA gene sequence
  • accession: JX458401
  • database: nuccore

GC content

  • @ref: 43699
  • GC-content: 49.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43699

culture collection no.: CECT 8581, LMG 28208

literature

  • topic: Phylogeny
  • Pubmed-ID: 26782963
  • title: Ampullimonas aquatilis gen. nov., sp. nov. isolated from bottled mineral water.
  • authors: Franca L, Albuquerque L, Sanchez C, Fareleira P, da Costa MS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000903
  • year: 2016

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43699Luís Franca, Luciana Albuquerque, Claudia Sánchez, Paula Fareleira, Milton S. da CostaAmpullimonas aquatilis gen. nov., sp. nov. isolated from bottled mineral water10.1099/ijsem.0.000903IJSEM 66: 1459-1465 201626782963
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/