Strain identifier

BacDive ID: 140870

Type strain: Yes

Species: Terasakiella brassicae

Strain Designation: B3

NCBI tax ID(s): 1634917 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 43694

BacDive-ID: 140870

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, helical-shaped, colony-forming

description: Terasakiella brassicae B3 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from Wastewater collected from a pickle-processing factory.

NCBI tax id

  • NCBI tax id: 1634917
  • Matching level: species

doi: 10.13145/bacdive140870.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Terasakiellaceae
  • genus: Terasakiella
  • species: Terasakiella brassicae
  • full scientific name: Terasakiella brassicae Han et al. 2016

@ref: 43694

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodospirillales

family: Methylocystaceae

genus: Terasakiella

species: Terasakiella brassicae

strain designation: B3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
43694negative1.5-4 µm0.3-0.5 µmhelical-shapedyesmonotrichous, polar
69480yes93.541
69480negative100

colony morphology

  • @ref: 43694
  • colony size: 0.5-1 mm
  • colony color: non-pigmented
  • colony shape: circular
  • incubation period: 1 day
  • medium used: Marine agar 2216

Culture and growth conditions

culture medium

  • @ref: 43694
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43694positivegrowth4-40
43694positiveoptimum32-37mesophilic

culture pH

@refabilitytypepHPH range
43694positivegrowth5.5-9alkaliphile
43694positiveoptimum6.5-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43694
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.991

halophily

@refsaltgrowthtested relationconcentration
43694NaClpositivegrowth0.5-8 %(w/v)
43694NaClpositiveoptimum1-2 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4369416947citrate-assimilation
4369417754glycerol-assimilation
4369453423tween 40-assimilation
4369458187alginate-carbon source
4369422599arabinose-carbon source
4369417057cellobiose-carbon source
4369416947citrate-carbon source
4369415740formate-carbon source
4369428757fructose-carbon source
4369428260galactose-carbon source
4369424265gluconate-carbon source
4369417234glucose-carbon source
4369417754glycerol-carbon source
4369417716lactose-carbon source
4369417306maltose-carbon source
4369437684mannose-carbon source
4369430623oxalate-carbon source
4369426546rhamnose-carbon source
4369417992sucrose-carbon source
4369430929tartrate-carbon source
4369427082trehalose-carbon source
4369453423tween 40-carbon source
4369453426tween 80-carbon source
4369418222xylose-carbon source
4369417234glucose-fermentation
4369485146carboxymethylcellulose-hydrolysis
43694casein-hydrolysis
436944853esculin-hydrolysis
436945291gelatin-hydrolysis
4369428017starch-hydrolysis
4369418186tyrosine-hydrolysis
4369416199urea-hydrolysis
4369417632nitrate-reduction
4369416301nitrite-reduction
4369430089acetate+carbon source
4369417968butyrate+carbon source
4369424996lactate+carbon source
4369425115malate+carbon source
4369417272propionate+carbon source
4369415361pyruvate+carbon source
4369430031succinate+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
436942676amoxicillinyesyes10 µg (disc)
4369428971ampicillinyesyes10 µg (disc)
436943393carbenicillinyesyes100 µg (disc)
4369417698chloramphenicolyesyes30 µg (disc)
4369448923erythromycinyesyes15 µg (disc)
4369417833gentamicinyesyes10 µg (disc)
436946104kanamycinyesyes30 µg (disc)
436947507neomycinyesyes30 µg (disc)
43694100246norfloxacinyesyes10 µg (disc)
4369428368novobiocinyesyes30 µg (disc)
4369428077rifampicinyesyes5 µg (disc)
4369417076streptomycinyesyes10 µg (disc)
436949332sulfamethoxazoleyesyes300 µg (disc)
4369418208penicillin gyesyes10 Unit (disc)
4369427902tetracyclineyesyes30 µg (disc)
43694209807cefoxitinyesyes30 µg (disc)
4369428669bacitracinyesyes0.04 Unit (disc)
436948309polymyxin byesyes300 Unit (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4369415688acetoinno
4369435581indoleno
4369416136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
4369417234glucose-
4369415688acetoin-

enzymes

@refvalueactivityec
43694catalase-1.11.1.6
43694arginine dihydrolase-3.5.3.6
43694cytochrome oxidase+1.9.3.1
43694alkaline phosphatase+3.1.3.1
43694acid phosphatase+3.1.3.2
43694esterase+
43694lipase (C 14)+
43694naphthol-AS-BI-phosphohydrolase+

Isolation, sampling and environmental information

isolation

  • @ref: 43694
  • sample type: Wastewater collected from a pickle-processing factory
  • geographic location: Zhejiang Province
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_37696.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_458;96_2157;97_2604;98_27717;99_37696&stattab=map
  • Last taxonomy: Terasakiella brassicae subclade
  • 16S sequence: KP994391
  • Sequence Identity:
  • Total samples: 61
  • aquatic counts: 60
  • animal counts: 1

Sequence information

16S sequences

  • @ref: 43694
  • description: 16S rRNA gene sequence
  • accession: KP994391
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Terasakiella brassicae CGMCC 1.15254GCA_014641495scaffoldncbi1634917
66792Terasakiella brassicae strain CGMCC 1.152541634917.3wgspatric1634917

GC content

  • @ref: 43694
  • GC-content: 42.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes90.602yes
gram-positiveno99.327no
anaerobicno95.529yes
aerobicno51.131yes
halophileno64.311no
spore-formingno88.255no
glucose-utilno66.724yes
thermophileno89.826yes
motileyes93.504no
glucose-fermentno89.516no

External links

@ref: 43694

culture collection no.: KCTC 42652, CGMCC 1.15254

literature

  • topic: Phylogeny
  • Pubmed-ID: 26828022
  • title: Terasakiella brassicae sp. nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella.
  • authors: Han SB, Su Y, Hu J, Wang RJ, Sun C, Wu D, Zhu XF, Wu M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000946
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Food Industry, Hydroxybutyrates/chemistry, Methylocystaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, Waste Water/*microbiology
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43694Shuai-Bo Han, Yue Su, Jing Hu, Rui-Jun Wang, Cong Sun, Dildar Wu, Xu-Fen Zhu, Min WuTerasakiella brassicae sp. nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella10.1099/ijsem.0.000946IJSEM 66: 1807-1812 201626828022
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1