Strain identifier

BacDive ID: 140856

Type strain: Yes

Species: Aquisalinus flavus

Strain Designation: D11M-2

NCBI tax ID(s): 1526572 (species)

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General

@ref: 43668

BacDive-ID: 140856

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Aquisalinus flavus D11M-2 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from A surface water sample of the saline Lake Dasugan, depth 2.6 m.

NCBI tax id

  • NCBI tax id: 1526572
  • Matching level: species

doi: 10.13145/bacdive140856.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Parvularculales
  • family: Parvularculaceae
  • genus: Aquisalinus
  • species: Aquisalinus flavus
  • full scientific name: Aquisalinus flavus Zhong et al. 2016

@ref: 43668

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Parvularculales

family: Parvularculaceae

genus: Aquisalinus

species: Aquisalinus flavus

strain designation: D11M-2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
43668negative1.2-1.6 µm0.5-0.7 µmrod-shapedyesmonotrichous, polar
69480negative99.998

colony morphology

  • @ref: 43668
  • colony size: 0.1-0.4 mm
  • colony color: Yellow
  • colony shape: circular
  • incubation period: 4 days
  • medium used: MA agar

pigmentation

  • @ref: 43668
  • production: yes
  • name: Carotenoid Pigments

Culture and growth conditions

culture medium

  • @ref: 43668
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43668positivegrowth10-35
43668positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
43668positivegrowth6-10.5alkaliphile
43668positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 43668
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
43668no
69481no100
69480no99.985

halophily

@refsaltgrowthtested relationconcentration
43668NaClpositivegrowth0-14 %(w/v)
43668NaClpositiveoptimum2 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4366827689decanoate-assimilation
4366816899D-mannitol-assimilation
4366818401phenylacetate-assimilation
4366816947citrate-carbon source
4366815824D-fructose-carbon source
4366817924D-sorbitol-carbon source
4366865327D-xylose-carbon source
4366815428glycine-carbon source
4366817561L-cysteine-carbon source
4366818019L-lysine-carbon source
4366817716lactose-carbon source
4366817306maltose-carbon source
4366817790methanol-carbon source
4366815361pyruvate-carbon source
4366830031succinate-carbon source
4366827082trehalose-carbon source
4366816947citrate-energy source
4366815824D-fructose-energy source
4366817924D-sorbitol-energy source
4366865327D-xylose-energy source
4366815428glycine-energy source
4366817561L-cysteine-energy source
4366818019L-lysine-energy source
4366817716lactose-energy source
4366817306maltose-energy source
4366817790methanol-energy source
4366815361pyruvate-energy source
4366830031succinate-energy source
4366827082trehalose-energy source
4366817634D-glucose-fermentation
43668casein-hydrolysis
4366816467L-arginine-hydrolysis
4366817895L-tyrosine-hydrolysis
4366853424tween 20-hydrolysis
4366853423tween 40-hydrolysis
4366853425tween 60-hydrolysis
4366853426tween 80-hydrolysis
4366816199urea-hydrolysis
4366817632nitrate-reduction
4366817128adipate+assimilation
4366825115malate+assimilation
43668506227N-acetylglucosamine+assimilation
4366832032potassium gluconate+assimilation
4366812936D-galactose+carbon source
4366817634D-glucose+carbon source
4366816024D-mannose+carbon source
4366830849L-arabinose+carbon source
4366829985L-glutamate+carbon source
4366817992sucrose+carbon source
4366812936D-galactose+energy source
4366817634D-glucose+energy source
4366816024D-mannose+energy source
4366830849L-arabinose+energy source
4366829985L-glutamate+energy source
4366817992sucrose+energy source
436684853esculin+hydrolysis
436685291gelatin+hydrolysis
4366828017starch+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4366848923erythromycinyesyes15 µg (disc)
4366817698chloramphenicolyesyes30 µg (disc)
4366817076streptomycinyesyes10 µg (disc)
4366828971ampicillinyesyes10 µg (disc)
436683732clarithromycinyesyes15 µg (disc)
436686104kanamycinyesyes30 µg (disc)
4366828001vancomycinyesyes30 µg (disc)
43668100246norfloxacinyesyes10 µg (disc)
436689332sulfamethoxazoleyesyes300 µg (disc)
4366827902tetracyclineyesyes30 µg (disc)
4366817833gentamicinyesyes10 µg (disc)
436683745clindamycinyesyes2 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4366835581indoleno
4366816136hydrogen sulfideyes

enzymes

@refvalueactivityec
43668alkaline phosphatase+3.1.3.1
43668esterase (C 4)+
43668esterase Lipase (C 8)+
43668leucine arylamidase+3.4.11.1
43668trypsin+3.4.21.4
43668alpha-chymotrypsin+3.4.21.1
43668acid phosphatase+3.1.3.2
43668naphthol-AS-BI-phosphohydrolase+
43668alpha-glucosidase+3.2.1.20
43668valine arylamidase+/-
43668cystine arylamidase+/-3.4.11.3
43668beta-glucosidase+/-3.2.1.21
43668lipase (C 14)-
43668alpha-galactosidase-3.2.1.22
43668beta-galactosidase-3.2.1.23
43668beta-glucuronidase-3.2.1.31
43668N-acetyl-beta-glucosaminidase-3.2.1.52
43668alpha-mannosidase-3.2.1.24
43668alpha-fucosidase-3.2.1.51
43668catalase+1.11.1.6
43668cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 43668
  • sample type: A surface water sample of the saline Lake Dasugan, depth 2.6 m
  • geographic location: Qaidam Baisin, Qinghai Province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 38.88
  • longitude: 93.955

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Surface water

taxonmaps

  • @ref: 69479
  • File name: preview.99_127321.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_406;96_34570;97_61632;98_82048;99_127321&stattab=map
  • Last taxonomy: Aquisalinus flavus
  • 16S sequence: KJ782430
  • Sequence Identity:
  • Total samples: 66
  • soil counts: 7
  • aquatic counts: 45
  • animal counts: 11
  • plant counts: 3

Sequence information

16S sequences

  • @ref: 43668
  • description: 16S rRNA gene sequence
  • accession: KJ782430
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aquisalinus flavus CGMCC 1.12921GCA_014640095contigncbi1526572
66792Aquisalinus flavus D11M-2GCA_014534785scaffoldncbi1526572
66792Aquisalinus flavus strain CGMCC 1.129211526572.5wgspatric1526572
66792Aquisalinus flavus D11M-2GCA_022559625chromosomencbi1526572

GC content

  • @ref: 43668
  • GC-content: 59
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno77.99no
gram-positiveno97.715yes
anaerobicno99.194yes
aerobicyes92.818yes
halophileno66.325no
spore-formingno96.382yes
motileyes89.722yes
glucose-fermentno90.04yes
thermophileno95.906no
glucose-utilyes83.769no

External links

@ref: 43668

culture collection no.: CGMCC 1.12921, KCTC 42673

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26843074Aquisalinus flavus gen. nov., sp. nov., a member of the family Parvularculaceae isolated from a saline lake.Zhong ZP, Liu Y, Wang F, Zhou YG, Liu HC, Liu ZPInt J Syst Evol Microbiol10.1099/ijsem.0.0009502016Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lakes/*microbiology, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, Pigmentation, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNAGenetics
Phylogeny34705626Reclassification of Parvularcula flava as Aquisalinus luteolus nom. nov. and emended description of the genus Aquisalinus.Ying JJ, Wu ZC, Fang YC, Xu L, Sun CInt J Syst Evol Microbiol10.1099/ijsem.0.0050722021Alphaproteobacteria/*classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43668Zhi-Ping Zhong, Ying Liu, Fang Wang, Yu-Guang Zhou, Hong-Can Liu, Zhi-Pei LiuAquisalinus flavus gen. nov., sp. nov., a member of the family Parvularculaceae isolated from a saline lake10.1099/ijsem.0.000950IJSEM 66: 1813-1817 201626843074
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1