Strain identifier

BacDive ID: 140816

Type strain: Yes

Species: Aquaticitalea lipolytica

Strain Designation: Ar-125

Strain history: Y. Wu; Second Inst. of Oceanogr., State Oceanic Admin., China; Ar-125.

NCBI tax ID(s): 1247562 (species)

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General

@ref: 43604

BacDive-ID: 140816

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Aquaticitalea lipolytica Ar-125 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from seawater from Prydz Bay.

NCBI tax id

  • NCBI tax id: 1247562
  • Matching level: species

strain history

  • @ref: 67770
  • history: Y. Wu; Second Inst. of Oceanogr., State Oceanic Admin., China; Ar-125.

doi: 10.13145/bacdive140816.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Aquaticitalea
  • species: Aquaticitalea lipolytica
  • full scientific name: Aquaticitalea lipolytica Xamxidin et al. 2016

@ref: 43604

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Aquaticitalea

species: Aquaticitalea lipolytica

strain designation: Ar-125

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
43604negative1.5-2.5 µm0.2-0.3 µmrod-shapednogliding
69480negative99.988

colony morphology

  • @ref: 43604
  • colony size: 1-2 mm
  • colony color: Yellow
  • colony shape: circular
  • incubation period: 5 days
  • medium used: MA agar

Culture and growth conditions

culture medium

  • @ref: 43604
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43604positivegrowth15-37
43604positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
43604positivegrowth6.5-8
43604positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43604
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

halophily

@refsaltgrowthtested relationconcentration
43604NaClpositivegrowth0.5-5 %(w/v)
43604NaClpositiveoptimum0.5-1 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4360436219alpha-lactose-builds acid from
4360417057cellobiose-builds acid from
4360415824D-fructose-builds acid from
4360412936D-galactose-builds acid from
4360416024D-mannose-builds acid from
436046731melezitose-builds acid from
4360416988D-ribose-builds acid from
4360465327D-xylose-builds acid from
4360416236ethanol-builds acid from
4360430849L-arabinose-builds acid from
4360462345L-rhamnose-builds acid from
4360429864mannitol-builds acid from
4360417268myo-inositol-builds acid from
4360416634raffinose-builds acid from
4360415963ribitol-builds acid from
4360430911sorbitol-builds acid from
4360427922sorbose-builds acid from
4360417992sucrose-builds acid from
4360427082trehalose-builds acid from
4360417234glucose-fermentation
4360417632nitrate-reduction
4360416947citrate+assimilation
4360417234glucose+builds acid from
4360417306maltose+builds acid from
43604peptone+carbon source
43604tryptone+carbon source
43604yeast extract+carbon source
43604peptone+energy source
43604tryptone+energy source
43604yeast extract+energy source
4360416991dna+hydrolysis
436044853esculin+hydrolysis
436045291gelatin+hydrolysis
4360428017starch+hydrolysis
4360453423tween 40+hydrolysis
4360453425tween 60+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4360428971ampicillinyesyes10 µg (disc)
436042676amoxicillinyesyes20 µg (disc)
4360429007ceftriaxoneyesyes30 µg (disc)
4360417698chloramphenicolyesyes30 µg (disc)
4360448923erythromycinyesyes15 µg (disc)
4360471415nitrofurantoinyesyes300 µg (disc)
4360428368novobiocinyesyes30 µg (disc)
4360428077rifampicinyesyes5 µg (disc)
4360427902tetracyclineyesyes30 µg (disc)
4360428001vancomycinyesyes30 µg (disc)
436043534cephalexinyesyes30 µg (disc)
4360417833gentamicinyesyes10 µg (disc)
436046104kanamycinyesyes30 µg (disc)
4360450694minocyclineyesyes30 µg (disc)
436047507neomycinyesyes30 µg (disc)
4360417076streptomycinyesyes10 µg (disc)
4360428864tobramycinyesyes10 µg (disc)
4360418208penicillin gyesyes10 Unit (disc)
436048309polymyxin byesyes300 Unit (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4360416136hydrogen sulfideno
4360435581indoleno
4360415688acetoinyes

metabolite tests

  • @ref: 43604
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
43604catalase+1.11.1.6
43604cytochrome oxidase+1.9.3.1
43604acid phosphatase+3.1.3.2
43604alkaline phosphatase+3.1.3.1
43604alpha-chymotrypsin+3.4.21.1
43604cystine arylamidase+3.4.11.3
43604esterase (C 4)+
43604esterase Lipase (C 8)+
43604beta-glucosidase+3.2.1.21
43604leucine arylamidase+3.4.11.1
43604alpha-mannosidase+3.2.1.24
43604trypsin+3.4.21.4
43604valine arylamidase+
43604N-acetyl-beta-glucosaminidase-3.2.1.52
43604beta-D-fucosidase-3.2.1.38
43604alpha-galactosidase-3.2.1.22
43604beta-galactosidase-3.2.1.23
43604alpha-glucosidase-3.2.1.20
43604beta-glucuronidase-3.2.1.31
43604lipase (C 14)-
43604naphthol-AS-BI-phosphohydrolase-
43604arginine dihydrolase-3.5.3.6
43604lysine decarboxylase-4.1.1.18
43604ornithine decarboxylase-4.1.1.17
43604tryptophan deaminase-4.1.99.1
43604urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43604seawater from Prydz BayPrydz Bay, Cape DarnleyAntarcticaATAAustralia and Oceania-68.01170.515
67770Antarctic seawater

isolation source categories

Cat1Cat2Cat3
#Climate#Cold#Polar
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_15468.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_107;96_7924;97_9531;98_11704;99_15468&stattab=map
  • Last taxonomy: Aquaticitalea lipolytica subclade
  • 16S sequence: JX844498
  • Sequence Identity:
  • Total samples: 825
  • soil counts: 7
  • aquatic counts: 713
  • animal counts: 97
  • plant counts: 8

Sequence information

16S sequences

  • @ref: 43604
  • description: 16S rRNA gene sequence
  • accession: JX844498
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aquaticitalea lipolytica CGMCC 1.15295GCA_014641635scaffoldncbi1247562
66792Aquaticitalea lipolytica strain CGMCC 1.152951247562.3wgspatric1247562

GC content

  • @ref: 43604
  • GC-content: 31.8
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno95.43yes
gram-positiveno98.518yes
anaerobicno99.121yes
aerobicyes79.866yes
halophileno87.792yes
spore-formingno96.843no
thermophileno98.181yes
glucose-utilyes86.628no
motileno89.687no
glucose-fermentno89.535no

External links

@ref: 43604

culture collection no.: CGMCC 1.15295, JCM 30876, NRIC 0975

literature

  • topic: Phylogeny
  • Pubmed-ID: 29144229
  • title: Aquaticitalea lipolytica gen. nov., sp. nov., isolated from Antarctic seawater.
  • authors: Xamxidin M, Wu YH, Jian SL, Zhou YD, Wang CS, Tohty D, Xu XW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001101
  • year: 2016
  • mesh: Antarctic Regions, Bacterial Typing Techniques, Base Composition, Carotenoids/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Taiwan, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43604Maripat Xamxidin, Yue-Hong Wu, Shu-Ling Jian, Ya-Dong Zhou, Chun-Sheng Wang, Dilbar Tohty, Xue-Wei XuAquaticitalea lipolytica gen. nov., sp. nov., isolated from Antarctic seawater10.1099/ijsem.0.001101IJSEM 66: 2657-2663 201629144229
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1