Strain identifier

BacDive ID: 140779

Type strain: Yes

Species: Mycobacterium aquiterrae

Strain Designation: S-I-6

NCBI tax ID(s): 627680 (species)

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General

@ref: 43557

BacDive-ID: 140779

keywords: 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped, colony-forming

description: Mycobacterium aquiterrae S-I-6 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from groundwater.

NCBI tax id

  • NCBI tax id: 627680
  • Matching level: species

doi: 10.13145/bacdive140779.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium aquiterrae
  • full scientific name: Mycobacterium aquiterrae Lee and Whang 2017

@ref: 43557

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Mycobacteriales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium aquiterrae

strain designation: S-I-6

type strain: yes

Morphology

cell morphology

  • @ref: 43557
  • gram stain: positive
  • cell length: 1-1.2 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43557
  • colony size: 0.1-0.5 mm
  • colony color: Light Yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 43557
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43557positivegrowth10-37
43557positiveoptimum25mesophilic

culture pH

@refabilitytypepHPH range
43557positivegrowth4-9alkaliphile
43557positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43557
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43557
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43557NaClpositivegrowth0-5 %(w/v)
43557NaClpositiveoptimum2 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43557168082-dehydro-D-gluconate-assimilation
43557581435-dehydro-D-gluconate-assimilation
4355727613amygdalin-assimilation
4355716947citrate-assimilation
4355715963ribitol-assimilation
4355717108D-arabinose-assimilation
4355718333D-arabitol-assimilation
4355712936D-galactose-assimilation
4355717634D-glucose-assimilation
4355762318D-lyxose-assimilation
4355716443D-tagatose-assimilation
4355765327D-xylose-assimilation
4355717113erythritol-assimilation
4355728066gentiobiose-assimilation
4355715443inulin-assimilation
4355730849L-arabinose-assimilation
4355718403L-arabitol-assimilation
4355718287L-fucose-assimilation
4355717266L-sorbose-assimilation
4355765328L-xylose-assimilation
4355717716lactose-assimilation
4355717306maltose-assimilation
435576731melezitose-assimilation
43557320061methyl alpha-D-glucopyranoside-assimilation
4355743943methyl alpha-D-mannoside-assimilation
4355774863methyl beta-D-xylopyranoside-assimilation
43557506227N-acetylglucosamine-assimilation
4355730031succinate-assimilation
4355717151xylitol-assimilation
43557168082-dehydro-D-gluconate-builds acid from
43557581435-dehydro-D-gluconate-builds acid from
4355715963ribitol-builds acid from
4355717108D-arabinose-builds acid from
4355718333D-arabitol-builds acid from
4355728847D-fucose-builds acid from
4355712936D-galactose-builds acid from
4355762318D-lyxose-builds acid from
4355716899D-mannitol-builds acid from
4355716024D-mannose-builds acid from
4355717924D-sorbitol-builds acid from
4355716443D-tagatose-builds acid from
4355765327D-xylose-builds acid from
4355716813galactitol-builds acid from
4355724265gluconate-builds acid from
4355717754glycerol-builds acid from
4355717268myo-inositol-builds acid from
4355715443inulin-builds acid from
4355730849L-arabinose-builds acid from
4355718403L-arabitol-builds acid from
4355718287L-fucose-builds acid from
4355762345L-rhamnose-builds acid from
4355717266L-sorbose-builds acid from
4355765328L-xylose-builds acid from
435576731melezitose-builds acid from
4355728053melibiose-builds acid from
43557320061methyl alpha-D-glucopyranoside-builds acid from
4355743943methyl alpha-D-mannoside-builds acid from
4355774863methyl beta-D-xylopyranoside-builds acid from
4355716634raffinose-builds acid from
4355717151xylitol-builds acid from
4355716991dna-hydrolysis
435575291gelatin-hydrolysis
4355717368hypoxanthine-hydrolysis
4355717895L-tyrosine-hydrolysis
4355716199urea-hydrolysis
4355715318xanthine-hydrolysis
4355718305arbutin+assimilation
4355717057cellobiose+assimilation
4355715824D-fructose+assimilation
4355728847D-fucose+assimilation
4355716899D-mannitol+assimilation
4355716024D-mannose+assimilation
4355716988D-ribose+assimilation
4355717924D-sorbitol+assimilation
4355716813galactitol+assimilation
435574853esculin+assimilation
4355724265gluconate+assimilation
4355717754glycerol+assimilation
4355728087glycogen+assimilation
4355717268myo-inositol+assimilation
4355762345L-rhamnose+assimilation
4355728053melibiose+assimilation
4355716634raffinose+assimilation
4355717814salicin+assimilation
4355728017starch+assimilation
4355717992sucrose+assimilation
4355727082trehalose+assimilation
4355732528turanose+assimilation
4355727613amygdalin+builds acid from
4355718305arbutin+builds acid from
4355717057cellobiose+builds acid from
4355715824D-fructose+builds acid from
4355717634D-glucose+builds acid from
4355716988D-ribose+builds acid from
4355717113erythritol+builds acid from
435574853esculin+builds acid from
4355728066gentiobiose+builds acid from
4355728087glycogen+builds acid from
4355717716lactose+builds acid from
43557maltose hydrate+builds acid from
43557506227N-acetylglucosamine+builds acid from
4355717814salicin+builds acid from
4355728017starch+builds acid from
4355717992sucrose+builds acid from
4355727082trehalose+builds acid from
4355732528turanose+builds acid from
435574853esculin+hydrolysis
4355728017starch+hydrolysis
4355717632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4355729687teicoplaninyesyes30 µg (disc)
435572637amikacinyesyes30 µg (disc)
4355728971ampicillinyesyes20 µg (disc)
4355717833gentamicinyesyes10 µg (disc)
435576104kanamycinyesyes30 µg (disc)
435577507neomycinyesyes30 µg (disc)
4355728001vancomycinyesyes30 µg (disc)
4355748923erythromycinyesyes15 µg (disc)
4355785260spiramycinyesyes100 µg (disc)
4355728077rifampicinyesyes10 µg (disc)
4355727902tetracyclineyesyes30 µg (disc)
435572790apramycinyesyes30 µg (disc)
4355724753hygromycinyesyes30 µg (disc)
435579169sisomycinyesyes10 µg (disc)
4355717698chloramphenicolyesyes30 µg (disc)
435576472lincomycinyesyes15 µg (disc)
43557100147nalidixic acidyesyes30 µg (disc)
4355717076streptomycinyesyes10 µg (disc)
4355716869oleandomycinyesyes15 µg (disc)
435572676amoxicillinyesyes10 µg (disc)
435577660nystatinyesyes50 µg (disc)
4355727641cycloheximideyesyes30 µg (disc)
4355748844roxithromycinyesyes15 µg (disc)
4355717636sphingomyelinyesyes30 µg (disc)
4355780025salinomycinyesyes30 µg (disc)
435573371capreomycinyesyes30 µg (disc)
435575530gramicidin syesyes30 µg (disc)
4355728915fosfomycinyesyes30 µg (disc)
435572682amphotericin byesyes2 µg (disc)
4355717334penicillinyesyes10 Unit (disc)
435578309polymyxin byesyes100 Unit (disc)
4355728669bacitracinyesyes10 Unit (disc)

enzymes

@refvalueactivityec
43557cytochrome oxidase+1.9.3.1
43557esterase (C 4)+
43557esterase Lipase (C 8)+
43557leucine arylamidase+3.4.11.1
43557cystine arylamidase+3.4.11.3
43557trypsin+3.4.21.4
43557acid phosphatase+3.1.3.2
43557naphthol-AS-BI-phosphohydrolase+
43557alpha-galactosidase+3.2.1.22
43557N-acetyl-beta-glucosaminidase+3.2.1.52
43557catalase-1.11.1.6
43557alkaline phosphatase-3.1.3.1
43557lipase (C 14)-
43557valine arylamidase-
43557alpha-chymotrypsin-3.4.21.1
43557beta-galactosidase-3.2.1.23
43557beta-glucuronidase-3.2.1.31
43557alpha-glucosidase-3.2.1.20
43557beta-glucosidase-3.2.1.21
43557alpha-mannosidase-3.2.1.24
43557alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43557C15:0 anteiso17.1
    43557C17:0 anteiso22.3
    43557C17:1 anteiso ω9c1.4
    43557C14:00.6
    43557C16:03.7
    43557C17:02.5
    4355710-methyl C17:00.6
    43557C18:03
    43557C18:1ω7c / C18:1ω6c1.8
    43557C18:1ω9c1.1
    43557C14:0 iso3.2
    43557C15:0 iso18.8
    43557C16:0 iso16.8
    43557C16:1 iso H0.5
    43557C17:0 iso2.3
    43557C17:1 iso ω9c / C16:0 10-methyl1.1
    43557C18:0 iso1.1
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 4.5
  • library/peak naming table: TSBA40 4.10
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 43557
  • sample type: groundwater
  • geographic location: Daejeon
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Groundwater

Sequence information

16S sequences

@refdescriptionaccessiondatabase
4355716S rRNA geneFJ796421nuccore
4355716S-23S ITS sequenceLC126335nuccore

GC content

  • @ref: 43557
  • GC-content: 64.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43557

culture collection no.: KACC 17600, NBRC 109805, NCAIM B 02535

literature

  • topic: Phylogeny
  • Pubmed-ID: 28920836
  • title: Mycobacterium aquiterrae sp. nov., a rapidly growing bacterium isolated from groundwater.
  • authors: Lee JC, Whang KS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002261
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Groundwater/*microbiology, Multilocus Sequence Typing, Mycobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43557Jae-Chan Lee, Kyung-Sook WhangMycobacterium aquiterrae sp. nov., a rapidly growing bacterium isolated from groundwater10.1099/ijsem.0.002261IJSEM 67: 4104-4110 201728920836