Strain identifier

BacDive ID: 140777

Type strain: Yes

Species: Massilia glaciei

Strain Designation: B448-2

Strain history: Y. Liu; Inst. of Tibetan Plateau Res., CAS, China; B448-2.

NCBI tax ID(s): 1524097 (species)

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General

@ref: 43555

BacDive-ID: 140777

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Massilia glaciei B448-2 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from ice core from Muztagh Glacier, 5770 m above sea level.

NCBI tax id

  • NCBI tax id: 1524097
  • Matching level: species

strain history

  • @ref: 67770
  • history: Y. Liu; Inst. of Tibetan Plateau Res., CAS, China; B448-2.

doi: 10.13145/bacdive140777.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Massilia
  • species: Massilia glaciei
  • full scientific name: Massilia glaciei Gu et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Mokoshia glaciei

@ref: 43555

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia glaciei

strain designation: B448-2

type strain: yes

Morphology

cell morphology

  • @ref: 43555
  • gram stain: negative
  • cell length: 2.5-9.2 µm
  • cell width: 0.5-0.75 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43555
  • colony color: white, opaque
  • colony shape: circular
  • incubation period: 3-4 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 43555
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43555positivegrowth4-30
43555positiveoptimum20psychrophilic
67770positivegrowth15psychrophilic

culture pH

@refabilitytypepHPH range
43555positivegrowth5-9alkaliphile
43555positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43555
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43555NaClpositivegrowth0-1 %(w/v)
43555NaClpositiveoptimum0 %(w/v)non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4355517128adipate-assimilation
4355527689decanoate-assimilation
4355516947citrate-assimilation
4355517634D-glucose-assimilation
4355516899D-mannitol-assimilation
4355530849L-arabinose-assimilation
4355538030L-mannitol-assimilation
4355525115malate-assimilation
4355529864mannitol-assimilation
4355537684mannose-assimilation
43555506227N-acetylglucosamine-assimilation
4355518401phenylacetate-assimilation
4355515963ribitol-builds acid from
4355517057cellobiose-builds acid from
4355515824D-fructose-builds acid from
4355517634D-glucose-builds acid from
4355516899D-mannitol-builds acid from
4355516024D-mannose-builds acid from
4355565327D-xylose-builds acid from
4355528260galactose-builds acid from
4355517754glycerol-builds acid from
4355515443inulin-builds acid from
4355530849L-arabinose-builds acid from
4355562345L-rhamnose-builds acid from
4355517306maltose-builds acid from
435556731melezitose-builds acid from
4355528053melibiose-builds acid from
4355517268myo-inositol-builds acid from
4355516634raffinose-builds acid from
4355527922sorbose-builds acid from
4355517992sucrose-builds acid from
4355527082trehalose-builds acid from
4355517234glucose-fermentation
435555291gelatin-hydrolysis
43555911224-nitrophenyl alpha-D-glucopyranoside+assimilation
4355517306maltose+assimilation
435554853esculin+builds acid from
4355528066gentiobiose+builds acid from
4355517716lactose+builds acid from
435554853esculin+hydrolysis
4355517632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
4355535581indoleno
4355515688acetoinyes

metabolite tests

  • @ref: 43555
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
43555cytochrome oxidase+1.9.3.1
43555catalase+1.11.1.6
43555arginine dihydrolase-3.5.3.6
43555urease-3.5.1.5
43555alkaline phosphatase+3.1.3.1
43555esterase (C 4)+
43555esterase Lipase (C 8)+
43555leucine arylamidase+3.4.11.1
43555valine arylamidase+
43555acid phosphatase+3.1.3.2
43555naphthol-AS-BI-phosphohydrolase+
43555beta-galactosidase+3.2.1.23
43555alpha-glucosidase+3.2.1.20
43555N-acetyl-beta-glucosaminidase+3.2.1.52
43555lipase (C 14)-
43555cystine arylamidase-3.4.11.3
43555trypsin-3.4.21.4
43555alpha-chymotrypsin-3.4.21.1
43555alpha-galactosidase-3.2.1.22
43555beta-glucosidase-3.2.1.21
43555beta-glucuronidase-3.2.1.31

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43555ice core from Muztagh Glacier, 5770 m above sea levelMuztagh Glacier on the Tibetan PlateauChinaCHNAsia36.34987.173
67770Ice core from the Muztagh Glacier, on the Tibetan PlateauTibetChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Glacier
#Environmental#Terrestrial#Core sample

Sequence information

16S sequences

  • @ref: 43555
  • description: 16S rRNA gene sequence
  • accession: KJ755877
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Massilia glaciei B448-22791355050draftimg1524097
67770Massilia glaciei B448-2GCA_003011895contigncbi1524097

GC content

  • @ref: 67770
  • GC-content: 66.1
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 43555

culture collection no.: JCM 30271, CGMCC 1.12920, CCM 8861

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28901899Massilia glaciei sp. nov., isolated from the Muztagh Glacier.Gu Z, Liu Y, Xu B, Wang N, Jiao N, Shen L, Liu H, Zhou Y, Liu X, Li J, Sun JInt J Syst Evol Microbiol10.1099/ijsem.0.0022522017Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome
Phylogeny29851375Massilia violaceinigra sp. nov., a novel purple-pigmented bacterium isolated from glacier permafrost.Wang H, Zhang X, Wang S, Zhao B, Lou K, Xing XHInt J Syst Evol Microbiol10.1099/ijsem.0.0028262018Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Permafrost/*microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome
Phylogeny31140962Massilia atriviolacea sp. nov., a dark purple-pigmented bacterium isolated from soil.Yang E, Zhao M, Li S, Wang Y, Sun L, Liu J, Wang WInt J Syst Evol Microbiol10.1099/ijsem.0.0034492019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/isolation & purification, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome
Phylogeny33864512Massilia aromaticivorans sp. nov., a BTEX Degrading Bacterium Isolated from Arctic Soil.Son J, Lee H, Kim M, Kim DU, Ka JOCurr Microbiol10.1007/s00284-021-02379-y2021Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids, Nucleic Acid Hybridization, Oxalobacteraceae, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *SoilTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43555Zhengquan Gu, Yongqin Liu, Baiqing Xu, Ninglian Wang, Nianzhi Jiao, Liang Shen, Hongcan Liu, Yuguang Zhou, Xiaobo Liu, Jiule Li, Jia SunMassilia glaciei sp. nov., isolated from the Muztagh Glacier10.1099/ijsem.0.002252IJSEM 67: 4075-4079 201728901899
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/