Strain identifier

BacDive ID: 140708

Type strain: Yes

Species: Brumimicrobium aurantiacum

Strain Designation: N62

Strain history: <- Zongjun Du, Shandong Univ..

NCBI tax ID(s): 1737063 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 43481

BacDive-ID: 140708

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Brumimicrobium aurantiacum N62 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from marine sediment of the coast of Weihai.

NCBI tax id

  • NCBI tax id: 1737063
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Zongjun Du, Shandong Univ..

doi: 10.13145/bacdive140708.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Crocinitomicaceae
  • genus: Brumimicrobium
  • species: Brumimicrobium aurantiacum
  • full scientific name: Brumimicrobium aurantiacum Zhang et al. 2017

@ref: 43481

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Crocinitomicaceae

genus: Brumimicrobium

species: Brumimicrobium aurantiacum

strain designation: N62

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43481negative0.8-1.5 µm0.3-0.4 µmrod-shapedno
69480negative99.998

colony morphology

  • @ref: 43481
  • colony size: 0.6-1 mm
  • colony color: opaque, orange
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Marine agar 2216

Culture and growth conditions

culture medium

  • @ref: 43481
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43481positiveoptimum28-30mesophilic
43481positivegrowth4-40
67771positivegrowth28-33mesophilic

culture pH

@refabilitytypepHPH range
43481positiveoptimum7-7.5
43481positivegrowth5.5-9alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43481facultative anaerobe
67771aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.995

halophily

@refsaltgrowthtested relationconcentration
43481NaClpositiveoptimum2-3 %(w/v)
43481NaClpositivegrowth0.5-13 %(w/v)

observation

  • @ref: 43481
  • observation: no growth without NaCl

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4348116947citrate-assimilation
434812509agar-hydrolysis
4348158187alginate-hydrolysis
4348185146carboxymethylcellulose-hydrolysis
4348128017starch-hydrolysis
4348116988D-ribose+builds acid from
43481potassium 5-dehydro-D-gluconate+builds acid from
4348116651(S)-lactate+carbon source
4348117925alpha-D-glucose+carbon source
4348115824D-fructose+carbon source
4348112936D-galactose+carbon source
4348118024D-galacturonic acid+carbon source
434818391D-gluconate+carbon source
4348116899D-mannitol+carbon source
4348133801D-saccharate+carbon source
4348132323glucuronamide+carbon source
4348116977L-alanine+carbon source
4348118287L-fucose+carbon source
4348162345L-rhamnose+carbon source
4348117716lactose+carbon source
4348117306maltose+carbon source
4348126490quinate+carbon source
4348116634raffinose+carbon source
4348117992sucrose+carbon source
4348127082trehalose+carbon source
4348132528turanose+carbon source
4348116651(S)-lactate+energy source
4348117925alpha-D-glucose+energy source
4348115824D-fructose+energy source
4348112936D-galactose+energy source
4348118024D-galacturonic acid+energy source
434818391D-gluconate+energy source
4348116899D-mannitol+energy source
4348133801D-saccharate+energy source
4348132323glucuronamide+energy source
4348116977L-alanine+energy source
4348118287L-fucose+energy source
4348162345L-rhamnose+energy source
4348117716lactose+energy source
4348117306maltose+energy source
4348126490quinate+energy source
4348116634raffinose+energy source
4348117992sucrose+energy source
4348127082trehalose+energy source
4348132528turanose+energy source
434815291gelatin+hydrolysis
4348153424tween 20+hydrolysis
4348153423tween 40+hydrolysis
4348153425tween 60+hydrolysis
4348153426tween 80+hydrolysis
4348116651(S)-lactate+oxidation
4348117925alpha-D-glucose+oxidation
4348115824D-fructose+oxidation
4348112936D-galactose+oxidation
4348118024D-galacturonic acid+oxidation
434818391D-gluconate+oxidation
4348116899D-mannitol+oxidation
4348133801D-saccharate+oxidation
4348132323glucuronamide+oxidation
4348116977L-alanine+oxidation
4348118287L-fucose+oxidation
4348162345L-rhamnose+oxidation
4348117716lactose+oxidation
4348117306maltose+oxidation
4348126490quinate+oxidation
4348116634raffinose+oxidation
4348117992sucrose+oxidation
4348127082trehalose+oxidation
4348132528turanose+oxidation
4348117632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4348148923erythromycinyesyes15 µg (disc)
4348117334penicillinyesyes10 µg (disc)
4348117698chloramphenicolyesyes30 µg (disc)
4348128077rifampicinyesyes5 µg (disc)
434816472lincomycinyesyes2 µg (disc)
4348129007ceftriaxoneyesyes30 µg (disc)
4348131168acetylspiramycinyesyes30 µg (disc)
4348128971ampicillinyesyes10 µg (disc)
43481204928cefotaximeyesyes30 µg (disc)
434813745clindamycinyesyes30 µg (disc)
4348128864tobramycinyesyes10 µg (disc)
4348127902tetracyclineyesyes30 µg (disc)
43481100246norfloxacinyesyes10 µg (disc)
434817507neomycinyesyes30 µg (disc)
4348117833gentamicinyesyes10 µg (disc)
4348153727sulfamethoxydiazineyesyes10 µg (disc)
434817731ofloxacinyesyes5 µg (disc)
434816104kanamycinyesyes30 µg (disc)
4348117076streptomycinyesyes10 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4348115688acetoinno
4348116136hydrogen sulfideno
4348135581indoleno

metabolite tests

  • @ref: 43481
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
43481catalase+1.11.1.6
43481cytochrome oxidase-1.9.3.1
43481gelatinase+
43481arginine dihydrolase-3.5.3.6
43481lysine decarboxylase-4.1.1.18
43481ornithine decarboxylase-4.1.1.17
43481urease-3.5.1.5
43481tryptophan deaminase-4.1.99.1
43481alkaline phosphatase+3.1.3.1
43481esterase (C 4)+
43481esterase Lipase (C 8)+
43481lipase (C 14)+
43481leucine arylamidase+3.4.11.1
43481valine arylamidase+
43481cystine arylamidase+3.4.11.3
43481trypsin+3.4.21.4
43481alpha-chymotrypsin+3.4.21.1
43481acid phosphatase+3.1.3.2
43481naphthol-AS-BI-phosphohydrolase+
43481alpha-galactosidase-3.2.1.22
43481beta-glucosidase-3.2.1.21
43481beta-galactosidase-3.2.1.23
43481beta-glucuronidase-3.2.1.31
43481alpha-glucosidase-3.2.1.20
43481N-acetyl-beta-glucosaminidase-3.2.1.52
43481alpha-mannosidase-3.2.1.24
43481beta-D-fucosidase-3.2.1.38

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43481marine sediment of the coast of WeihaiCoast of WeihaiChinaCHNAsia36.909122.254
67771From marine solar salternChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Coast

Sequence information

16S sequences

  • @ref: 43481
  • description: 16S rRNA gene sequence
  • accession: KR528475
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brumimicrobium aurantiacum N62GCA_003402975scaffoldncbi1737063
66792Brumimicrobium aurantiacum N622831584504draftimg1737063

GC content

  • @ref: 43481
  • GC-content: 35.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno87.394no
flagellatedno95.447yes
gram-positiveno97.942yes
anaerobicno97.919yes
aerobicyes88.311no
halophileyes63.115no
spore-formingno94.039no
glucose-utilyes82.63yes
thermophileno99.607yes
glucose-fermentno91.323no

External links

@ref: 43481

culture collection no.: KCTC 42589, MCCC 1H00117

literature

  • topic: Phylogeny
  • Pubmed-ID: 28829037
  • title: Brumimicrobium aurantiacum sp. nov., isolated from coastal sediment.
  • authors: Zhang H, Han JR, Shi MJ, Du ZJ, Chen GJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002092
  • year: 2017
  • mesh: Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43481Hui Zhang, Ji-Ru Han, Ming-Jing Shi, Zong-Jun Du, Guan-Jun ChenBrumimicrobium aurantiacum sp. nov., isolated from coastal sediment10.1099/ijsem.0.002092IJSEM 67: 3256-3260 201728829037
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1