Strain identifier

BacDive ID: 140561

Type strain: Yes

Species: Hymenobacter tenuis

Strain Designation: POB6

Strain history: <- Chi Nam Seong, Sunchon National Univ.

NCBI tax ID(s): 2232097 (species)

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General

@ref: 43292

BacDive-ID: 140561

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Hymenobacter tenuis POB6 is an aerobe, mesophilic, Gram-negative bacterium that forms irregular colonies and was isolated from wastewater sample collected from the acidic water neutralisation facility of Gwanyang steelworks.

NCBI tax id

  • NCBI tax id: 2232097
  • Matching level: species

strain history

@refhistory
67770C. N. Seong; Sunchon Natl. Univ., South Korea; POB6.
67771<- Chi Nam Seong, Sunchon National Univ.

doi: 10.13145/bacdive140561.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Hymenobacteraceae
  • genus: Hymenobacter
  • species: Hymenobacter tenuis
  • full scientific name: Hymenobacter tenuis Kang et al. 2017

@ref: 43292

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Hymenobacteraceae

genus: Hymenobacter

species: Hymenobacter tenuis

strain designation: POB6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43292negative1.8-2.8 µm0.4 µmrod-shapedno
67771rod-shapedno
67771negative

colony morphology

  • @ref: 43292
  • colony size: 2-7 mm
  • colony color: red-pink
  • colony shape: irregular
  • incubation period: 3 days
  • medium used: R2A agar (Becton Dickinson)

pigmentation

@refproductionname
43292noFlexirubin
43292yesCarotenoid pigments

Culture and growth conditions

culture medium

@refnamegrowth
43292R2A agar (Becton Dickinson)yes
43292R2A (Becton Dickinson)yes
43292nutrient (NA; Becton Dickinson)yes
43292plate count agar (PCA; Becton Dickinson)yes
43292glucose yeast extract agar (GYEA, Gordon RE 1962)yes
43292marine agar (MA, Becton-Dickinson)no
43292TSA (Becton Dickinson)no

culture temp

@refgrowthtypetemperaturerange
43292nogrowth37mesophilic
43292nogrowth4psychrophilic
43292positivemaximum30mesophilic
43292positivegrowth10-35
67770positivegrowth25mesophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
43292positivegrowth6-10alkaliphile
43292positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43292aerobe
67771aerobe

spore formation

  • @ref: 43292
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43292NaClpositivegrowth0-0.5 %
43292NaClpositivemaximum0 %
43292NaClnogrowth0.6-5 %

observation

@refobservation
43292production of extracellular polymeric substances
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4329217632nitrate+reduction
4329235581indole-
432924853esculin+hydrolysis
43292casein+hydrolysis
4329216991dna+hydrolysis
432925291gelatin+hydrolysis
4329228017starch+hydrolysis
4329253424tween 20+hydrolysis
4329253311sodium alginate-hydrolysis
4329217029chitin-hydrolysis
4329285146carboxymethylcellulose-hydrolysis
4329217368hypoxanthine-hydrolysis
4329217309pectin-hydrolysis
4329217895L-tyrosine-hydrolysis
4329253426tween 80-hydrolysis
4329216199urea-hydrolysis
4329215318xanthine-hydrolysis
4329237166xylan-hydrolysis
4329222599arabinose+assimilation
4329217234glucose+assimilation
4329217306maltose+assimilation
4329237684mannose+assimilation
4329217992sucrose+builds acid from
4329226546rhamnose+/-builds acid from
4329228757fructose-builds acid from
4329217306maltose-builds acid from
4329217234glucose-builds acid from
4329228260galactose-builds acid from
4329217716lactose-builds acid from
4329237684mannose-builds acid from
4329218222xylose-builds acid from
4329227082trehalose-builds acid from
4329229016arginine-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
432922637amikacinyesyes30 µg (disc)
4329228971ampicillinyesyes10 µg (disc)
4329217698chloramphenicolyesyes30 µg (disc)
4329248923erythromycinyesyes15 µg (disc)
4329217833gentamicinyesyes10 µg (disc)
432926104kanamycinyesyes30 µg (disc)
43292100147nalidixic acidyesyes30 µg (disc)
4329217334penicillinyesyes10 Unit
4329217076streptomycinyesyes10 µg (disc)
4329227902tetracyclineyesyes30 µg (disc)
4329228001vancomycinyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4329235581indoleno
4329216136hydrogen sulfideno

enzymes

@refvalueactivityec
43292catalase+1.11.1.6
43292cytochrome oxidase+1.9.3.1
43292acid phosphatase+3.1.3.2
43292alkaline phosphatase+3.1.3.1
43292esterase (C 4)+
43292leucine arylamidase+3.4.11.1
43292naphthol-AS-BI-phosphohydrolase+
43292N-acetyl-beta-glucosaminidase+3.2.1.52
43292valine arylamidase+
43292alpha-chymotrypsin-3.4.21.1
43292cystine arylamidase-3.4.11.3
43292alpha-fucosidase-3.2.1.51
43292alpha-galactosidase-3.2.1.22
43292beta-galactosidase-3.2.1.23
43292beta-glucuronidase-3.2.1.31
43292lipase (C 14)-
43292alpha-mannosidase-3.2.1.24
43292trypsin-3.4.21.4

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43292C14:02
    43292C16:08.6
    43292C18:02
    43292C15:0 iso18.3
    43292C16:0 iso1.1
    43292C17:0 iso2.9
    43292iso-15:0 3-OH1.1
    43292iso-17:0 3-OH1
    43292C15:0 anteiso9.2
    43292C17:0 anteiso1
    43292C16:1 iso H1.7
    43292C16:1 ω5c13.7
    43292C18:1ω9c1.5
    43292C16:1 ω7c / C16:1 ω6c15.9
    43292iso C17:1 I / anteiso-C17:1 B18.3
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A (Becton Dickinson)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
43292wastewater sample collected from the acidic water neutralisation facility of Gwanyang steelworksJeonnam ProvinceRepublic of KoreaKORAsiaR2A-Agar (Becton Dickinson)5 days25
67770Wastewater treatment facilityRepublic of KoreaKORAsia
67771From wastewater, acidic water neutralization facility of Gwangyang steelworksJeonnam ProvinceRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Condition#Acidic
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_28480.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_263;96_1043;97_2946;98_21298;99_28480&stattab=map
  • Last taxonomy: Hymenobacter
  • 16S sequence: KY196418
  • Sequence Identity:
  • Total samples: 1158
  • soil counts: 219
  • aquatic counts: 215
  • animal counts: 710
  • plant counts: 14

Sequence information

16S sequences

  • @ref: 43292
  • description: Hymenobacter sp. strain POB6 16S ribosomal RNA gene, partial sequence
  • accession: KY196418
  • length: 1445
  • database: nuccore
  • NCBI tax ID: 1898978

GC content

@refGC-contentmethod
6777161.0
6777061thermal denaturation, midpoint method (Tm)

External links

@ref: 43292

culture collection no.: KCTC 52271, JCM 31659

literature

  • topic: Phylogeny
  • Pubmed-ID: 28691666
  • title: Hymenobacter tenuis sp. nov., isolated from wastewater of an acidic water neutralization facility.
  • authors: Kang JW, Lee JH, Choe HN, Seong CN
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001918
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Cytophagaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistry, Waste Water/*microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43292Joo Won Kang, Ji Hee Lee, Han Na Choe and Chi Nam SeongHymenobacter tenuis sp. nov., isolated from wastewater of an acidic water neutralization facility10.1099/ijsem.0.001918IJSEM 67: 2171-2177 201728691666
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/