Strain identifier

BacDive ID: 140546

Type strain: Yes

Species: Thiorhodococcus alkaliphilus

Strain Designation: JA878

Strain history: <- Ch Sasikala, JNT Univ, India

NCBI tax ID(s): 1752873 (species)

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General

@ref: 43334

BacDive-ID: 140546

keywords: 16S sequence, Bacteria, anaerobe, organotroph|photoautotroph, mesophilic, Gram-negative, motile, coccus-shaped

description: Thiorhodococcus alkaliphilus JA878 is an anaerobe, organotroph|photoautotroph, mesophilic bacterium that was isolated from sediment of a brown pond near Nari Salt Pan, Bhavnagar, Gujarat, India.

NCBI tax id

  • NCBI tax id: 1752873
  • Matching level: species

strain history

@refhistory
67770Ch. Sasikala; Jawaharlal Nehru Technol. Univ., India; JA878.
67771<- Ch Sasikala, JNT Univ, India

doi: 10.13145/bacdive140546.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Chromatiaceae
  • genus: Thiorhodococcus
  • species: Thiorhodococcus alkaliphilus
  • full scientific name: Thiorhodococcus alkaliphilus Prathyash Ushus et al. 2017

@ref: 43334

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Chromatiaceae

genus: Thiorhodococcus

species: Thiorhodococcus alkaliphilus

strain designation: JA878

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityflagellum arrangement
43334negative2-4 µmcoccus-shapedyespolar
67771yesmonotrichous, polar
67771negative

pigmentation

@refproductionname
43334yesBacteriochlorophyll alpha
43334yesRhodopin

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
43334positiveoptimum27mesophilic
43334positivegrowth25-30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43334positiveoptimum10-11alkaliphile
43334positivegrowth6-12

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: anaerobe

nutrition type

@reftype
43334organotroph|photoautotroph
43334photolithoautotroph

halophily

@refsaltgrowthtested relationconcentration
43334NaClpositiveoptimum1-3 %(w/v)
43334NaClpositivegrowth1-6 %(w/v)

observation

@refobservation
43334reddish brown when grown phototrophically
43334quinone Q-8
67770quinones: Q-8
67771quinones: Q-8(90%), MK-8(4%)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4333416411acetic acid+carbon source
4333415361pyruvate+carbon source
4333428757fructose+carbon source
4333429806fumarate+carbon source
4333425115malate+carbon source
4333430031succinate+carbon source
4333435899crotonate+carbon source
4333431011valerate+carbon source
4333486477sodium sulfite+
4333433403elemental sulfur+
43334132112sodium thiosulfate+
4333416811methionine+
4333428300glutamine+nitrogen source
4333435391aspartate+nitrogen source
4333429987glutamate+nitrogen source
4333417632nitrate+nitrogen source
4333416301nitrite+nitrogen source
4333431206ammonium chloride+nitrogen source
4333416150benzoate-carbon source
43334casamino acids-carbon source
4333425646octanoate-carbon source
4333415740formate-carbon source
4333424996lactate-carbon source
4333417272propionate-carbon source
4333430929tartrate-carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation date
43334sediment of a brown pond near Nari Salt Pan, Bhavnagar, Gujarat, IndiaBhavnagar, GujaratIndiaINDAsiaKH2PO4 (0.5 g/L), CaCl2.2H2O (0.15 g/L), MgSO4.7H2O (2 g/L), NH4Cl (0.6 g/L), yeast extract (0.3 g/L), NaCl (20 g/L), sodium pyruvate (3 g/L), ferric citrate [5 ml/L] from 0.1 % (w/v) stock] and trace element solution SL7 (1 ml/L)7 days272014-10-18
67770Sediment sample of a brown pond near Nari Salt PanBhavnagar, GujaratIndiaINDAsia
67771From sediment sample, brown pond near Nari Salt PanBhavnagar, GujaratIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Pond (small)
#Environmental#Aquatic#Sediment

Sequence information

16S sequences

  • @ref: 43334
  • description: 16S rRNA gene sequence
  • accession: LN901453
  • database: nuccore

GC content

  • @ref: 43334
  • GC-content: 62.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43334

culture collection no.: KCTC 15531, JCM 31245

literature

  • topic: Phylogeny
  • Pubmed-ID: 28693683
  • title: Description of a phototrophic bacterium, Thiorhodococcus alkaliphilus sp. nov.
  • authors: Prathyash Ushus MJ, Divyasree B, Lakshmi KVNS, Dave BP, Sasikala C, Ramana CV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001947
  • year: 2017
  • mesh: Bacterial Typing Techniques, Bacteriochlorophyll A/chemistry, Carotenoids/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, India, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thiotrichaceae/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry, *Water Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43334M. J. Prathyash Ushus, B. Divyasree, K. V. N. S. Lakshmi, Bharti P. Dave, Ch. Sasikala, Ch. V. RamanaDescription of a phototrophic bacterium, Thiorhodococcus alkaliphilus sp. nov.10.1099/ijsem.0.001947IJSEM 67: 2323-2327 201728693683
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc