Strain identifier

BacDive ID: 140472

Type strain: Yes

Species: Psychromonas aquatilis

Strain Designation: M1A1

Strain history: CIP <- 2016, S. Glaeser, Giessen Univ., Giessen, Germany: strain M1A1

NCBI tax ID(s): 2005072 (species)

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General

@ref: 43204

BacDive-ID: 140472

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Psychromonas aquatilis M1A1 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water samples.

NCBI tax id

  • NCBI tax id: 2005072
  • Matching level: species

strain history

  • @ref: 116443
  • history: CIP <- 2016, S. Glaeser, Giessen Univ., Giessen, Germany: strain M1A1

doi: 10.13145/bacdive140472.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Psychromonadaceae
  • genus: Psychromonas
  • species: Psychromonas aquatilis
  • full scientific name: Psychromonas aquatilis Kämpfer et al. 2017

@ref: 43204

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Psychromonadaceae

genus: Psychromonas

species: Psychromonas aquatilis

strain designation: M1A1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43204negative3 µm1.2 µmrod-shapedyes
116443negativeoval-shapedno

colony morphology

  • @ref: 43204
  • colony color: beige-white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: tryptone soya agar (TSA-1, Oxoid)

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
43204tryptone soya agar (TSA-1, Oxoid)yessupplemented with 1 % (w/v) NaCl
43204marine agar (MA)yes
43204tryptone soy agar (TSA)yes
43204Columbia agar with sheep blood (BA; Liofilchem)yes
43204thiosulfate-citrate-bile salts agar (TCBS; Oxoid)no
43204nutritient agar (NA)no
43204MacConkey agarno
116443CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
43204positiveoptimum30mesophilic
43204positiveoptimum5-22psychrophilic
43204positivegrowth4-28

culture pH

@refabilitytypepH
43204positivegrowth6.5-8.0
43204positiveoptimum6.5-7.0
43204nogrowth5
43204nogrowth8.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43204
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
43204NaClpositivegrowth0-7.0 %(w/v)
43204NaClpositiveoptimum0-3.0 %(w/v)
43204NaClnogrowth8 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4320430849L-arabinose-builds acid from
4320432032potassium gluconate-builds acid from
4320425115malate-builds acid from
43204506227N-acetylglucosamine-assimilation
4320427689decanoate-assimilation
4320417128adipate-assimilation
4320453258sodium citrate-assimilation
4320418401phenylacetate-assimilation
432044853esculin+hydrolysis
4320428017starch+hydrolysis
432045291gelatin-hydrolysis
4320415824D-fructose-carbon source
4320412936D-galactose-carbon source
4320417634D-glucose-carbon source
4320416024D-mannose-carbon source
4320416899D-mannitol-carbon source
4320422599arabinose-carbon source
4320465327D-xylose-carbon source
4320417057cellobiose-carbon source
4320417716lactose-carbon source
4320417306maltose-carbon source
4320417992sucrose-carbon source
4320429806fumarate-carbon source
4320424996lactate-carbon source
4320425115malate-carbon source
4320417924D-sorbitol-carbon source
4320417268myo-inositol-fermentation
4320427082trehalose-fermentation

metabolite production

  • @ref: 43204
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43204catalase+1.11.1.6
43204cytochrome oxidase+1.9.3.1
43204arginine dihydrolase-3.5.3.6
43204beta-galactosidase-3.2.1.23
43204alkaline phosphatase+3.1.3.1
43204leucine arylamidase+3.4.11.1
43204acid phosphatase+3.1.3.2
43204naphthol-AS-BI-phosphohydrolase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43204C12:04.2
    43204C16:025.2
    43204C16:1ω7c55.9
    43204C18:1ω7c4.6
    43204C12:0 aldehyd/iso-C16:1/C14:0 3-OH10.1
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy agar (TSA)
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • system: MIS MIDI
  • instrument: 5898A, Hewlett Packard
  • method/protocol: Kämpfer & Kroppenstedt 1996
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation date
43204water samplesKing George Island, AntarcticaChileCHLMiddle and South America-62-58tryptone soya agar (Oxoid)supplemented with 1 % (w/v) NaCl1 week18
116443Environment, Antarctic sea waterAntarcticChileCHLMiddle and South America2014-03-01

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

  • @ref: 116443
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 43204
  • description: Psychromonas sp. strain M1A1 16S ribosomal RNA gene, partial sequence
  • accession: KX645876
  • length: 1471
  • database: nuccore
  • NCBI tax ID: 2005072

GC content

  • @ref: 43204
  • GC-content: 38.5
  • method: Thermal denaturation, fluorometry

External links

@ref: 43204

culture collection no.: CIP 111183, CCM 8710, LMG 29766

straininfo link

  • @ref: 96727
  • straininfo: 412652

literature

  • topic: Phylogeny
  • Pubmed-ID: 28109208
  • title: Psychromonas aquatilis sp. nov., isolated from seawater samples obtained in the Chilean Antarctica.
  • authors: Kampfer P, Irgang R, Poblete-Morales M, Glaeser SP, Cortez-San Martin M, Avendano-Herrera R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001801
  • year: 2017
  • mesh: Antarctic Regions, Bacterial Typing Techniques, Base Composition, Chile, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43204Peter Kämpfer, Rute Irgang, Matías Poblete-Morales, Stefanie P. Glaeser, Marcelo Cortez-San Martín, Ruben Avendaño-HerreraPsychromonas aquatilis sp. nov., isolated from seawater samples obtained in the Chilean Antarctica10.1099/ijsem.0.001801IJSEM 67: 1306-1311 201728109208
96727Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID412652.1
116443Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111183Collection of Institut Pasteur (CIP 111183)