Strain identifier
BacDive ID: 139768
Type strain: 
Species: Pseudomonas aeruginosa
Strain Designation: NN2, clone C40A
Strain history: <- J. Klockgether, Hannover Medical School, Germany; NN2
NCBI tax ID(s): 287 (species)
version 9 (current version)
General
@ref: 22000
BacDive-ID: 139768
DSM-Number: 29238
keywords: Bacteria, human pathogen
description: Pseudomonas aeruginosa NN2 is a human pathogen that was isolated from airways of a cystic fibrosis patient.
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
- @ref: 22000
- history: <- J. Klockgether, Hannover Medical School, Germany; NN2
doi: 10.13145/bacdive139768.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 22000
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Burkholderiales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900
strain designation: NN2, clone C40A
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 22000 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| 22000 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | yes | https://mediadive.dsmz.de/medium/381 | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
culture temp
- @ref: 22000
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 22000 | + | - | - | + | + | - | + | - | + | - | - | + | + | - | + | + | + | + | + | - | + |
| 22000 | - | - | - | + | + | - | + | - | + | - | - | + | + | - | + | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 22000
- sample type: airways of a cystic fibrosis patient
- geographic location: Hannover
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body-Site | #Oral cavity and airways | #Airways |
| #Infection | #Disease | #Cystic fibrosis |
| #Infection | #Patient |
Safety information
risk assessment
- @ref: 22000
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 22000
culture collection no.: DSM 29238
straininfo link
- @ref: 96213
- straininfo: 396883
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22000 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29238 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29238) | |
| 68369 | Automatically annotated from API 20NE | |||
| 96213 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID396883.1 |