Strain identifier

BacDive ID: 139661

Type strain: No

Species: Bacillus pseudomycoides

Strain Designation: NRS-233

Strain history: CIP <- 1998, L.K. Nakamura, NCAUR, Peoria, Illinoi, USA: strain NRRL B-346 <- N.R. Smith: strain NRS-233 <- American Museum Natural History <- W.W. Ford & J.S. Lawrence

NCBI tax ID(s): 64104 (species)

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General

@ref: 42258

BacDive-ID: 139661

keywords: Bacteria, facultative anaerobe, spore-forming, Gram-positive, rod-shaped

description: Bacillus pseudomycoides NRS-233 is a facultative anaerobe, spore-forming, Gram-positive bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 64104
  • Matching level: species

strain history

  • @ref: 42258
  • history: CIP <- 1998, L.K. Nakamura, NCAUR, Peoria, Illinoi, USA: strain NRRL B-346 <- N.R. Smith: strain NRS-233 <- American Museum Natural History <- W.W. Ford & J.S. Lawrence

doi: 10.13145/bacdive139661.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus pseudomycoides
  • full scientific name: Bacillus pseudomycoides Nakamura 1998

@ref: 42258

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus pseudomycoides

strain designation: NRS-233

type strain: no

Morphology

cell morphology

  • @ref: 42258
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 42258
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
42258MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
42258CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
42258CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
42258positivegrowth30
42258positivegrowth22-37
42258nogrowth10
42258nogrowth45
42258nogrowth55

culture pH

  • @ref: 42258
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 42258
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 42258
  • spore formation: yes

halophily

  • @ref: 42258
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
422584853esculin+hydrolysis
42258606565hippurate-hydrolysis
4225817632nitrate+reduction
4225816301nitrite-reduction
4225817632nitrate+respiration

metabolite production

  • @ref: 42258
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
4225815688acetoin+
4225817234glucose+

enzymes

@refvalueactivityec
42258oxidase-
42258beta-galactosidase+3.2.1.23
42258alcohol dehydrogenase-1.1.1.1
42258gelatinase+
42258amylase+
42258DNase+
42258catalase+1.11.1.6
42258tween esterase+
42258gamma-glutamyltransferase-2.3.2.2
42258lecithinase+
42258lipase-
42258lysine decarboxylase-4.1.1.18
42258ornithine decarboxylase-4.1.1.17
42258tryptophan deaminase-
42258urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
42258-+++-+---+++---+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
42258++-+--+++++----+--++-----------+-----------------+---------+------------+--+-+-++--+--+--++--++-++-

Safety information

risk assessment

  • @ref: 42258
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 42258

culture collection no.: CIP 105701, NRRL B-346

straininfo link

  • @ref: 96121
  • straininfo: 67403

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42258Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105701Collection of Institut Pasteur (CIP 105701)
68382Automatically annotated from API zym
96121Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67403.1