Strain identifier
BacDive ID: 139538
Type strain: 
Species: Bacillus subtilis
Strain Designation: RS2008
Strain history: CIP <- 2009, A. Sekowska, Inst. Pasteur, Paris, France: strain RS2008 <- INRA, Jouy en Josas, France
NCBI tax ID(s): 1423 (species)
version 8.1 (current version)
General
@ref: 41887
BacDive-ID: 139538
keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, motile, rod-shaped
description: Bacillus subtilis RS2008 is a facultative anaerobe, mesophilic, Gram-positive bacterium of the family Bacillaceae.
NCBI tax id
- NCBI tax id: 1423
- Matching level: species
strain history
- @ref: 41887
- history: CIP <- 2009, A. Sekowska, Inst. Pasteur, Paris, France: strain RS2008 <- INRA, Jouy en Josas, France
doi: 10.13145/bacdive139538.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus subtilis
- full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Vibrio subtilis
@ref: 41887
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Bacillus
species: Bacillus subtilis
strain designation: RS2008
type strain: no
Morphology
cell morphology
- @ref: 41887
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 41887 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
| 41887 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
- @ref: 41887
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 41887
- oxygen tolerance: facultative anaerobe
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 41887 | 17632 | nitrate | + | reduction |
| 41887 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 41887
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 41887 | oxidase | + | |
| 41887 | catalase | + | 1.11.1.6 |
| 41887 | urease | + | 3.5.1.5 |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | + | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | - | |
| 68382 | cystine arylamidase | + | 3.4.11.3 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | + | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 41887 | - | + | + | + | - | + | - | + | - | - | + | + | + | + | - | + | + | - | - | - |
Isolation, sampling and environmental information
isolation
| @ref | geographic location | country | origin.country | continent | sample type | isolation date |
|---|---|---|---|---|---|---|
| 41887 | Paris | France | FRA | Europe | ||
| 41887 | Paris | France | FRA | Europe | Strain 168, C. Anagnostopoulos | 1995 |
Safety information
risk assessment
- @ref: 41887
- biosafety level: 1
- biosafety level comment: Risk group (French classification)
External links
@ref: 41887
culture collection no.: CIP 109935
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 41887 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109935 | Collection of Institut Pasteur (CIP 109935) | |
| 68382 | Automatically annotated from API zym |