Strain identifier

BacDive ID: 139527

Type strain: No

Species: Enterococcus faecalis

Strain history: CIP <- 1984, ATCC <- M. Bushby: strain CN 478, Streptococcus faecalis <- S.D. Elliot

NCBI tax ID(s): 1351 (species)

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General

@ref: 41824

BacDive-ID: 139527

keywords: Bacteria, facultative anaerobe, Gram-positive, coccus-shaped

description: Enterococcus faecalis CIP 100750 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium of the family Enterococcaceae.

NCBI tax id

  • NCBI tax id: 1351
  • Matching level: species

strain history

  • @ref: 41824
  • history: CIP <- 1984, ATCC <- M. Bushby: strain CN 478, Streptococcus faecalis <- S.D. Elliot

doi: 10.13145/bacdive139527.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecalis
  • full scientific name: Enterococcus faecalis (Andrewes and Horder 1906) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecalis

@ref: 41824

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecalis

type strain: no

Morphology

cell morphology

  • @ref: 41824
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 41824

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41824MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
41824CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
41824CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
41824positivegrowth37
41824positivegrowth30-45
41824nogrowth10

Physiology and metabolism

oxygen tolerance

  • @ref: 41824
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 41824
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
418244853esculin+hydrolysis
41824606565hippurate+hydrolysis
4182417632nitrate-reduction
4182416301nitrite-reduction

metabolite tests

  • @ref: 41824
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
41824oxidase-
41824beta-galactosidase+3.2.1.23
41824alcohol dehydrogenase+1.1.1.1
41824catalase-1.11.1.6
41824gamma-glutamyltransferase-2.3.2.2
41824lysine decarboxylase-4.1.1.18
41824ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41824--++-+---+++-+-+++--

Safety information

risk assessment

  • @ref: 41824
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 41824

culture collection no.: CIP 100750, ATCC 33186, WDCM 00210

straininfo link

  • @ref: 96013
  • straininfo: 46748

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity24112733Survival of Enterococcus faecalis during alkaline stress: changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts.Ran S, He Z, Liang JArch Oral Biol10.1016/j.archoralbio.2013.08.0132013Analysis of Variance, Biofilms/*growth & development, Cell Wall/pathology/*physiology/ultrastructure, Culture Techniques, Dentin/*microbiology, *Enterococcus faecalis/genetics/physiology/ultrastructure, Humans, *Hydrogen-Ion Concentration, Hydrophobic and Hydrophilic Interactions, Microscopy, Electron, Scanning, Sodium-Potassium-Exchanging ATPase/*metabolism, Transcription, Genetic/*physiology, Up-RegulationStress
Metabolism25990488Exploration of the mechanisms of biofilm formation by Enterococcus faecalis in glucose starvation environments.Ran SJ, Jiang W, Zhu CL, Liang JPAust Dent J10.1111/adj.123242015Biofilms, Enterococcus faecalis/metabolism/*physiology, Glucose/*metabolism, Humans, Hydrophobic and Hydrophilic Interactions, Microscopy, Confocal, Root Canal Therapy, Treatment Failure, Up-RegulationPathogenicity
Enzymology27706660Preliminary study on total protein extraction methods from Enterococcus faecalis biofilm.Chen W, Liang J, He Z, Jiang WGenet Mol Res10.4238/gmr.150389882016Bacterial Proton-Translocating ATPases/*isolation & purification/metabolism, *Biofilms, Electrophoresis, Polyacrylamide Gel, Enterococcus faecalis/*physiology, SonicationPhylogeny
Pathogenicity29653134The optimal ethanol lock therapy regimen for treatment of biofilm-associated catheter infections: an in-vitro study.Alonso B, Perez-Granda MJ, Rodriguez-Huerta A, Rodriguez C, Bouza E, Guembe MJ Hosp Infect10.1016/j.jhin.2018.04.0072018Bacteria/drug effects/growth & development, Biofilms/*drug effects/growth & development, Candida/drug effects/growth & development, Catheter-Related Infections/*prevention & control, Catheters/*microbiology, Disinfectants/*administration & dosage, Ethanol/*administration & dosage, Heparin/administration & dosage, Humans, Infection Control/*methods, Metabolism/drug effects, Staining and Labeling/methods, Tetrazolium Salts/metabolismMetabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41824Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20100750Collection of Institut Pasteur (CIP 100750)
68382Automatically annotated from API zym
96013Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID46748.1