Strain identifier

BacDive ID: 139058

Type strain: No

Species: Enterococcus faecalis

Strain Designation: 228

Strain history: CIP <- 1978, Fournier Lab., Clichy, France: strain 228

NCBI tax ID(s): 1351 (species)

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General

@ref: 41089

BacDive-ID: 139058

keywords: Bacteria, facultative anaerobe, Gram-positive, coccus-shaped

description: Enterococcus faecalis 228 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium of the family Enterococcaceae.

NCBI tax id

  • NCBI tax id: 1351
  • Matching level: species

strain history

@refhistory
410891978, Lab. Fournier, Clichy, France: strain 228
41089CIP <- 1978, Fournier Lab., Clichy, France: strain 228

doi: 10.13145/bacdive139058.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecalis
  • full scientific name: Enterococcus faecalis (Andrewes and Horder 1906) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecalis

@ref: 41089

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecalis

strain designation: 228

type strain: no

Morphology

cell morphology

  • @ref: 41089
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 41089
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41089MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
41089CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
41089CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperature
41089positivegrowth37
41089positivegrowth10-45

Physiology and metabolism

oxygen tolerance

  • @ref: 41089
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 41089
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
410894853esculin+hydrolysis
41089606565hippurate-hydrolysis
4108917632nitrate-reduction
4108916301nitrite-reduction

metabolite tests

  • @ref: 41089
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
41089oxidase-
41089beta-galactosidase-3.2.1.23
41089alcohol dehydrogenase+1.1.1.1
41089catalase-1.11.1.6
41089gamma-glutamyltransferase-2.3.2.2
41089lysine decarboxylase-4.1.1.18
41089ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41089-+++-+-+-+++---+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
41089---------------------------------------------------------------------------------------------------

Safety information

risk assessment

  • @ref: 41089
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 41089

culture collection no.: CIP 78.14

straininfo link

  • @ref: 95642
  • straininfo: 68273

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41089Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2078.14Collection of Institut Pasteur (CIP 78.14)
68382Automatically annotated from API zym
95642Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID68273.1