Strain identifier

BacDive ID: 1389

Type strain: Yes

Species: Pontibacillus litoralis

Strain history: <- X.-L. Cui, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China

NCBI tax ID(s): 1385512 (strain), 516703 (species)

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General

@ref: 15495

BacDive-ID: 1389

DSM-Number: 21186

keywords: 16S sequence, Bacteria, facultative anaerobe, moderately halophilic, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Pontibacillus litoralis DSM 21186 is a facultative anaerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from surface-sterilized sea anemone Anthopleura xanthogrammica from tidal flat.

NCBI tax id

NCBI tax idMatching level
1385512strain
516703species

strain history

  • @ref: 15495
  • history: <- X.-L. Cui, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China

doi: 10.13145/bacdive1389.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Pontibacillus
  • species: Pontibacillus litoralis
  • full scientific name: Pontibacillus litoralis Chen et al. 2010

@ref: 15495

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Pontibacillus

species: Pontibacillus litoralis

full scientific name: Pontibacillus litoralis Chen et al. 2010

type strain: yes

Morphology

cell morphology

  • @ref: 23020
  • gram stain: positive
  • cell length: 3.5-5.0 µm
  • cell width: 0.4-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 23020
  • colony size: 1.0-2.0 mm
  • colony color: creamy white
  • colony shape: circular
  • incubation period: 2-5 days
  • medium used: MA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15495MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d)yeshttps://mediadive.dsmz.de/medium/514dName: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d) Composition: Difco marine broth 37.4 g/l Agar 15.0 g/l Malt extract 1.0 g/l Soy peptone 1.0 g/l Pancreatic digest of casein 1.0 g/l Distilled water
23020Marine agar (MA)yes

culture temp

@refgrowthtypetemperaturerange
15495positivegrowth30mesophilic
23020positivegrowth15.0-50.0
23020positiveoptimum35.0mesophilic

culture pH

@refabilitytypepHPH range
23020positivegrowth6.0-10.0alkaliphile
23020positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23020
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 23020
  • spore description: ellipsoidal,subterminal,in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23020moderately halophilic
23020NaClpositivegrowth0.5-15.0 %(w/v)
23020NaCloptimum2.0-5.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2302015963ribitol-builds acid from
2302012936D-galactose-builds acid from
2302016899D-mannitol-builds acid from
2302016024D-mannose-builds acid from
2302016988D-ribose-builds acid from
2302017924D-sorbitol-builds acid from
2302065327D-xylose-builds acid from
2302016813galactitol-builds acid from
2302017754glycerol-builds acid from
2302030849L-arabinose-builds acid from
2302062345L-rhamnose-builds acid from
2302017716lactose-builds acid from
230206731melezitose-builds acid from
2302028053melibiose-builds acid from
2302017268myo-inositol-builds acid from
2302016634raffinose-builds acid from
2302017814salicin-builds acid from
23020182404-hydroxy-L-proline-carbon source
2302030089acetate-carbon source
2302015963ribitol-carbon source
2302017968butyrate-carbon source
2302017057cellobiose-carbon source
2302016947citrate-carbon source
2302018333D-arabitol-carbon source
2302015824D-fructose-carbon source
2302012936D-galactose-carbon source
2302016024D-mannose-carbon source
2302016988D-ribose-carbon source
2302017924D-sorbitol-carbon source
2302065327D-xylose-carbon source
2302023652dextrin-carbon source
2302024265gluconate-carbon source
2302015428glycine-carbon source
2302030849L-arabinose-carbon source
2302016467L-arginine-carbon source
2302029985L-glutamate-carbon source
2302015971L-histidine-carbon source
2302017191L-isoleucine-carbon source
2302015603L-leucine-carbon source
2302016643L-methionine-carbon source
2302017203L-proline-carbon source
2302062345L-rhamnose-carbon source
2302017115L-serine-carbon source
2302016414L-valine-carbon source
2302017716lactose-carbon source
230206731melezitose-carbon source
2302028053melibiose-carbon source
2302017268myo-inositol-carbon source
2302017272propionate-carbon source
2302016634raffinose-carbon source
2302017814salicin-carbon source
2302017992sucrose-carbon source
2302027082trehalose-carbon source
23020casein-hydrolysis
2302016991dna-hydrolysis
2302017368hypoxanthine-hydrolysis
2302028017starch-hydrolysis
2302053424tween 20-hydrolysis
2302053425tween 60-hydrolysis
2302053426tween 80-hydrolysis
2302016199urea-hydrolysis
2302015318xanthine-hydrolysis
2302016301nitrite-reduction
2302015824D-fructose+builds acid from
2302017634D-glucose+builds acid from
2302017306maltose+builds acid from
2302017992sucrose+builds acid from
2302027082trehalose+builds acid from
2302032528turanose+builds acid from
2302015824D-fructose+carbon source
2302017634D-glucose+carbon source
2302016899D-mannitol+carbon source
2302017754glycerol+carbon source
2302017306maltose+carbon source
2302017234glucose+fermentation
230204853esculin+hydrolysis
230205291gelatin+hydrolysis
2302053423tween 40+hydrolysis
2302016977L-alanine+nitrogen source
2302017196L-asparagine+nitrogen source
2302017632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantresistance conc.sensitivity conc.
23020100147nalidixic acidyesnoyes20 µg (disc)
2302028368novobiocinyesnoyes30 µg (disc)
230208309polymyxin byesnoyes30 µg (disc)
2302027902tetracyclineyesnoyes30 µg (disc)
2302017833gentamicinyesyes10 µg (disc)
2302017076streptomycinyesyes10 µg (disc)
230206472lincomycinyesyes2 µg (disc)
2302028971ampicillinyesyes30 µg (disc)
230203393carbenicillinyesyes30 µg (disc)
2302017698chloramphenicolyesyes30 µg (disc)
230206104kanamycinyesyes30 µg (disc)
2302028077rifampicinyesyes5 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2302035581indoleno
2302016136hydrogen sulfideno
2302015688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2302015688acetoin-
2302017234glucose+

enzymes

@refvalueactivityec
23020acid phosphatase-3.1.3.2
23020alkaline phosphatase+3.1.3.1
23020alpha-chymotrypsin-3.4.21.1
23020alpha-fucosidase-3.2.1.51
23020alpha-galactosidase-3.2.1.22
23020alpha-glucosidase-3.2.1.20
23020alpha-mannosidase-3.2.1.24
23020beta-galactosidase-3.2.1.23
23020beta-glucosidase-3.2.1.21
23020beta-glucuronidase-3.2.1.31
23020catalase+1.11.1.6
23020cystine arylamidase-3.4.11.3
23020cytochrome oxidase+1.9.3.1
23020esterase (C 4)+
23020esterase lipase (C 8)+
23020leucine arylamidase+3.4.11.1
23020lipase (C 14)-
23020lysine decarboxylase-4.1.1.18
23020N-acetyl-beta-glucosaminidase-3.2.1.52
23020naphthol-AS-BI-phosphohydrolase+
23020ornithine decarboxylase-4.1.1.17
23020phenylalanine deaminase-4.3.1.5
23020trypsin-3.4.21.4
23020valine arylamidase-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
15495surface-sterilized sea anemone Anthopleura xanthogrammica from tidal flatAnthopleura xanthogrammicaSouth China Sea, Naozhou IslandChinaCHNAsia
23020homogenates of a surface-sterilized sea anemone (Anthopleura xanthogrammica) collected from a tidal flatmarine agar 2216 (MA, pH 7.5; Difco)7 days37.0

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Tidal flat
#Host#Invertebrates (Other)#Cnidaria (Corals)

Safety information

risk assessment

  • @ref: 15495
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15495
  • description: Pontibacillus litoralis JSM 072002 16S ribosomal RNA gene, partial sequence
  • accession: EU583724
  • length: 1475
  • database: ena
  • NCBI tax ID: 1385512

GC content

  • @ref: 15495
  • GC-content: 41.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 15495

culture collection no.: DSM 21186, KCTC 13237, JSM 072002

straininfo link

  • @ref: 71040
  • straininfo: 405987

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19654352Pontibacillus litoralis sp. nov., a facultatively anaerobic bacterium isolated from a sea anemone, and emended description of the genus Pontibacillus.Chen YG, Zhang YQ, Yi LB, Li ZY, Wang YX, Xiao HD, Chen QH, Cui XL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.009910-02009Anaerobiosis, Anemone/*microbiology, Animals, Bacillaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyMetabolism
Phylogeny20833883Pontibacillus yanchengensis sp. nov., a moderately halophilic bacterium isolated from salt field soil.Yang Y, Zou Z, He M, Wang GInt J Syst Evol Microbiol10.1099/ijs.0.023911-02010Bacillaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Soil MicrobiologyMetabolism
Phylogeny25368138Pontibacillus salicampi sp. nov., a moderately halophilic bacterium isolated from saltern soil.Lee JC, Kim YS, Yun BS, Whang KSInt J Syst Evol Microbiol10.1099/ijs.0.066423-02014Bacillaceae/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Salinity, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15495Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21186)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21186
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23020Yi-Guang Chen,Yu-Qin Zhang,Lang-Bo Yi,Zhao-Yang Li,Yong-Xiao Wang,Huai-Dong Xiao,Qi-Hui Chen,Xiao-Long Cui,Wen-Jun Li10.1099/ijs.0.009910-0Pontibacillus litoralis sp. nov., a facultatively anaerobic bacterium isolated from a sea anemone, and emended description of the genus PontibacillusIJSEM 60: 560-565 201019654352
71040Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405987.1StrainInfo: A central database for resolving microbial strain identifiers