Strain identifier

BacDive ID: 138643

Type strain: No

Species: Pseudomonas putida

Strain Designation: 776.85

Strain history: CIP <- 1985, Lab. Ident. Inst. Pasteur, Paris, France: strain 776.85 <- Private Lab., Paris, France

NCBI tax ID(s): 303 (species)

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General

@ref: 40227

BacDive-ID: 138643

keywords: Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped

description: Pseudomonas putida 776.85 is an obligate aerobe, Gram-negative, motile bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 40227
  • history: CIP <- 1985, Lab. Ident. Inst. Pasteur, Paris, France: strain 776.85 <- Private Lab., Paris, France

doi: 10.13145/bacdive138643.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 40227

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

strain designation: 776.85

type strain: no

Morphology

cell morphology

  • @ref: 40227
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40227MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
40227CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
40227CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

  • @ref: 40227
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

oxygen tolerance

  • @ref: 40227
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4022717632nitrate-reduction
4022716301nitrite-reduction

metabolite production

  • @ref: 40227
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
40227oxidase+
40227catalase+1.11.1.6
40227urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
40227-++--+--+-++--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
40227ParisFranceFRAEurope
40227ParisFranceFRAEuropeHuman, Blood1985

Safety information

risk assessment

  • @ref: 40227
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 40227

culture collection no.: CIP 101936

straininfo link

  • @ref: 95323
  • straininfo: 69820

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40227Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101936Collection of Institut Pasteur (CIP 101936)
68382Automatically annotated from API zym
95323Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69820.1