Strain identifier

BacDive ID: 138579

Type strain: No

Species: Lysinibacillus sphaericus

Strain Designation: 2518

Strain history: CIP <- 1951, Proom, Wellcome C°, France: strain 2518

NCBI tax ID(s): 1421 (species)

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General

@ref: 40136

BacDive-ID: 138579

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Lysinibacillus sphaericus 2518 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Caryophanaceae.

NCBI tax id

  • NCBI tax id: 1421
  • Matching level: species

strain history

@refhistory
401361951, Proom, Wellcome C°, France: strain 2518
122820CIP <- strain CIP 51.25
40136CIP <- 1951, Proom, Wellcome C°, France: strain 2518

doi: 10.13145/bacdive138579.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Lysinibacillus
  • species: Lysinibacillus sphaericus
  • full scientific name: Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

  • synonyms

    @refsynonym
    20215Lysinibacillus varians
    20215Bacillus sphaericus
    20215Lysinibacillus mangiferahumi
    20215Lysinibacillus mangiferihumi
    20215Lysinibacillus tabacifolii

@ref: 40136

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Lysinibacillus

species: Lysinibacillus sphaericus

strain designation: 2518

type strain: no

Morphology

cell morphology

  • @ref: 40136
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 40136

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40136MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
40136CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
40136CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
40136positivegrowth30mesophilic
40136positivegrowth22-45
40136nogrowth10psychrophilic
40136nogrowth55thermophilic

culture pH

  • @ref: 40136
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 40136
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 40136
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
40136NaClpositivegrowth0-6 %
40136NaClnogrowth8 %
40136NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
40136citrate+carbon source16947
40136esculin-hydrolysis4853
40136hippurate-hydrolysis606565
40136nitrate-reduction17632
40136nitrite-reduction16301
40136nitrate-respiration17632
122820glucose-fermentation17234
122820inuline-fermentation2759
122820lactose-fermentation17716
122820nitrate-reduction17632
122820xylose-fermentation18222

metabolite production

  • @ref: 40136
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
4013615688acetoin-
4013617234glucose-
12282017234glucose-

enzymes

@refvalueactivityec
40136oxidase+
40136beta-galactosidase-3.2.1.23
40136alcohol dehydrogenase-1.1.1.1
40136gelatinase+/-
40136amylase-
40136DNase+
40136caseinase+3.4.21.50
40136catalase+1.11.1.6
40136tween esterase-
40136gamma-glutamyltransferase-2.3.2.2
40136lecithinase-
40136lipase-
40136lysine decarboxylase-4.1.1.18
40136ornithine decarboxylase-4.1.1.17
40136protease+
122820lecithinase-
40136urease-3.5.1.5
122820urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
40136-+++-+-+-++---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122820-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
40136------------------+-------------+------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

  • @ref: 122820
  • country: United States of America
  • origin.country: USA
  • continent: North America

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
1228201Risk group (French classification)
401361Risk group (French classification)

External links

@ref: 40136

culture collection no.: CIP 51.25, CRBIP3.2497

straininfo link

  • @ref: 95271
  • straininfo: 67439

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40136Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2051.25Collection of Institut Pasteur (CIP 51.25)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
95271Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67439.1
122820Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CRBIP3.2497Collection of Institut Pasteur (CRBIP3.2497)