Strain identifier

BacDive ID: 138534

Type strain: No

Species: Ensifer morelensis

Strain Designation: Lc04

Strain history: CIP <- 2003, E.T. Wang, Mexico: strain Lc04

NCBI tax ID(s): 106592 (species)

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General

@ref: 39957

BacDive-ID: 138534

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Ensifer morelensis Lc04 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Rhizobiaceae.

NCBI tax id

  • NCBI tax id: 106592
  • Matching level: species

strain history

@refhistory
399572003, E.T. Wang, Mexico: strain Lc04
39957CIP <- 2003, E.T. Wang, Mexico: strain Lc04

doi: 10.13145/bacdive138534.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Rhizobiaceae
  • genus: Ensifer
  • species: Ensifer morelensis
  • full scientific name: Ensifer morelensis corrig. (Wang et al. 2002) Wang et al. 2015

  • synonyms

    @refsynonym
    20215Sinorhizobium morelense
    20215Ensifer morelense

@ref: 39957

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Rhizobiaceae

genus: Ensifer

species: Ensifer adhaerens

strain designation: Lc04

type strain: no

Morphology

cell morphology

  • @ref: 39957
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 39957

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39957MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
39957CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
39957positivegrowth30mesophilic
39957positivegrowth25-37mesophilic
39957nogrowth10psychrophilic
39957nogrowth41thermophilic
39957nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 39957
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
39957NaClnogrowth0 %
39957NaClnogrowth2 %
39957NaClnogrowth4 %
39957NaClnogrowth6 %
39957NaClnogrowth8 %
39957NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
39957citrate-carbon source16947
39957esculin+hydrolysis4853
39957hippurate-hydrolysis606565
39957nitrate+reduction17632
39957nitrite+reduction16301
39957nitrate-respiration17632

antibiotic resistance

  • @ref: 39957
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 39957
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3995715688acetoin-
3995717234glucose-

enzymes

@refvalueactivityec
39957oxidase+
39957beta-galactosidase+3.2.1.23
39957alcohol dehydrogenase-1.1.1.1
39957gelatinase-
39957amylase-
39957DNase-
39957caseinase-3.4.21.50
39957catalase+1.11.1.6
39957tween esterase-
39957gamma-glutamyltransferase-2.3.2.2
39957lecithinase-
39957lipase-
39957lysine decarboxylase-4.1.1.18
39957ornithine decarboxylase-4.1.1.17
39957phenylalanine ammonia-lyase-4.3.1.24
39957protease-
39957tryptophan deaminase-
39957urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
39957-++--+--+-++---+-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
39957------------------------+/------------------+/-+/------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
39957++++++++++++++++++++++++++++++++++++++-+++------++++--++++-++-+++------+-+-+---++++++-+-++++-++-+++

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typeisolation date
39957MexicoMEXMiddle and South America
39957MexicoMEXNorth AmericaPlant, Root nodule of leguminous tree Leucaena leucocephala1996

Safety information

risk assessment

  • @ref: 39957
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 39957

culture collection no.: CIP 107894, LMG 21331, CFN E1007

straininfo link

  • @ref: 95238
  • straininfo: 27278

literature

  • topic: Phylogeny
  • Pubmed-ID: 12361275
  • title: Sinorhizobium morelense sp. nov., a Leucaena leucocephala-associated bacterium that is highly resistant to multiple antibiotics.
  • authors: Wang ET, Tan ZY, Willems A, Fernandez-Lopez M, Reinhold-Hurek B, Martinez-Romero E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-5-1687
  • year: 2002
  • mesh: Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Drug Resistance, Multiple, Bacterial/genetics, Fabaceae/microbiology, Molecular Sequence Data, Phenotype, Phylogeny, Plant Roots/microbiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sinorhizobium/*classification/drug effects/genetics/isolation & purification, Soil Microbiology
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
39957Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107894Collection of Institut Pasteur (CIP 107894)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
95238Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID27278.1