Strain identifier

BacDive ID: 138354

Type strain: Yes

Species: Marinospirillum megaterium

Strain Designation: H7

Strain history: CIP <- 2000, JCM <- T. Fujii: strain H7

NCBI tax ID(s): 64975 (species)

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General

@ref: 39535

BacDive-ID: 138354

keywords: 16S sequence, Bacteria, psychrophilic, Gram-negative, motile, rod-shaped

description: Marinospirillum megaterium H7 is a psychrophilic, Gram-negative, motile bacterium of the family Oceanospirillaceae.

NCBI tax id

  • NCBI tax id: 64975
  • Matching level: species

strain history

@refhistory
67770T. Fujii H7.
39535CIP <- 2000, JCM <- T. Fujii: strain H7

doi: 10.13145/bacdive138354.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Oceanospirillaceae
  • genus: Marinospirillum
  • species: Marinospirillum megaterium
  • full scientific name: Marinospirillum megaterium Satomi et al. 1998

@ref: 39535

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Oceanospirillaceae

genus: Marinospirillum

species: Marinospirillum megaterium

strain designation: H7

type strain: yes

Morphology

cell morphology

  • @ref: 39535
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39535MEDIUM 346 - for Marinospirillum megateriumyesDistilled water make up to (1000.000 ml);Sodium chloride (30.000 g);Agar(2.000 g);Tryptone (17.000 g);Yeast nitrogen base (1.000 g);Soytone peptone (3.000 g)
39535CIP Medium 346yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=346

culture temp

@refgrowthtypetemperaturerange
39535positivegrowth15psychrophilic
67770positivegrowth20psychrophilic
39535positivegrowth5-30
39535nogrowth37mesophilic
39535nogrowth41thermophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3953517632nitrate-reduction
3953516301nitrite-reduction

metabolite production

  • @ref: 39535
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
39535beta-galactosidase-3.2.1.23
39535catalase+1.11.1.6
39535gamma-glutamyltransferase-2.3.2.2
39535tryptophan deaminase-
39535urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
39535-----------+--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
39535Izu Islands, TokyoJapanJPNAsia
67770Izu IslandsJapanJPNAsiaKusaya-gravy
39535Izu Islands, TokyoJapanJPNAsiaFood, Kusaya gravy, fermented fish extract sauce1988

taxonmaps

  • @ref: 69479
  • File name: preview.99_84995.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15849;96_1225;97_1452;98_1770;99_84995&stattab=map
  • Last taxonomy: Marinospirillum megaterium
  • 16S sequence: AB006770
  • Sequence Identity:
  • Total samples: 115
  • soil counts: 20
  • aquatic counts: 7
  • animal counts: 80
  • plant counts: 8

Safety information

risk assessment

  • @ref: 39535
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Marinospirillum megaterium gene for 16S rRNA, partial sequence
  • accession: AB006770
  • length: 1456
  • database: ena
  • NCBI tax ID: 64975

GC content

  • @ref: 67770
  • GC-content: 44.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 39535

culture collection no.: CIP 106459, JCM 10129, CGMCC 1.2728

straininfo link

@refstraininfo
9510769233
95108312132

literature

  • topic: Phylogeny
  • Pubmed-ID: 9828435
  • title: Marinospirillum gen. nov., with descriptions of Marinospirillum megaterium sp. nov., isolated from kusaya gravy, and transfer of Oceanospirillum minutulum to Marinospirillum minutulum comb. nov.
  • authors: Satomi M, Kimura B, Hayashi M, Shouzen Y, Okuzumi M, Fujii T
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-48-4-1341
  • year: 1998
  • mesh: DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Food Microbiology, Gram-Negative Bacteria/*classification/*isolation & purification/physiology/ultrastructure, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Terminology as Topic
  • topic2: Biotechnology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
39535Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106459Collection of Institut Pasteur (CIP 106459)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
95107Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69233.1
95108Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID312132.1