Strain identifier

BacDive ID: 13824

Type strain: Yes

Species: Rhodobacter megalophilus

Strain Designation: JA194

Culture col. no.: DSM 18937, JCM 14598, KCTC 5602

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7795

BacDive-ID: 13824

DSM-Number: 18937

keywords: gram-negative, mesophilic, anaerobe, Bacteria, 16S sequence, genome sequence, ovoid-shaped

description: Rhodobacter megalophilus JA194 is an anaerobe, mesophilic, gram-negative bacterium that was isolated from soil sample collected from Suraj tal near Sarchu .

strain history: <- C. Sasikala, Centre Environment, Inst. Sci. and Technol., J. N. T. Univ., Hyderabad, India; JA194 <- K. Arunasri

doi: 10.13145/bacdive13824.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Rhodobacteraceae
  • genus: Rhodobacter
  • species: Rhodobacter megalophilus
  • full scientific name: Rhodobacter megalophilus Arunasri et al. 2008

@ref: 7795

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Rhodobacter

species: Rhodobacter megalophilus

full scientific name: Rhodobacter megalophilus Arunasri et al. 2008

strain designation: JA194

type strain: yes

Morphology

cell morphology

  • @ref: 32531
  • gram stain: negative
  • cell length: 1.5-2 µm
  • cell width: 1.2-1.5 µm
  • cell shape: ovoid-shaped
  • motility: no

pigmentation

  • @ref: 32531
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 7795
  • name: RHODOSPIRILLACEAE MEDIUM (modified) (DSMZ Medium 27), 28°C, + 0.3% Na pyruvate, anaerobic in the light
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf

culture temp

@refgrowthtypetemperaturerange
7795positivegrowth28mesophilic
32531positivegrowth05-40
32531positiveoptimum30-35mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7795anaerobe
32531aerobe

spore formation

  • @ref: 32531
  • spore formation: no

observation

  • @ref: 32531
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3253130089acetate+carbon source
3253135391aspartate+carbon source
3253116947citrate+carbon source
3253116236ethanol+carbon source
3253115740formate+carbon source
3253128757fructose+carbon source
3253129987glutamate+carbon source
3253117754glycerol+carbon source
3253125115malate+carbon source
3253117272propionate+carbon source
3253130911sorbitol+carbon source
3253131011valerate+carbon source

Isolation, sampling and environmental information

isolation

  • @ref: 7795
  • sample type: soil sample collected from Suraj tal near Sarchu (34° 17' N 77° 58' E, 11 975 feet above sea level)
  • geographic location: Indian Himalayas, between Jammu and Kashmir
  • country: India
  • origin.country: IND
  • continent: Asia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 7795
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7795
  • description: Rhodobacter aflagellatum partial 16S rRNA gene, type strain JA194T
  • accession: AM421024
  • length: 1406
  • database: ena
  • NCBI tax ID: 418630

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Rhodobacter megalophilus DSM 18937GCA_900188265scaffoldncbi418630
66792Rhodobacter megalophilus strain DSM 18937418630.3wgspatric418630
66792Rhodobacter megalophilus DSM 189372681813553permanentimg418630

GC content

@refGC-contentmethod
779566.67high performance liquid chromatography (HPLC)
3253166.67

External links

@ref: 7795

culture collection no.: DSM 18937, JCM 14598, KCTC 5602

straininfo link

@refpassport
20218http://www.straininfo.net/strains/833442
20218http://www.straininfo.net/strains/833443

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7795Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18937)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18937
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2874910.1099/ijs.0.65642-0
32531Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information28749
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)