Strain identifier

BacDive ID: 138087

Type strain: No

Species: Lactobacillus amylovorus

Strain history: CIP <- 1995, ATCC <- L.K. Nakamura, USA: strain NRRL B-4549 <- G. Hrubant

NCBI tax ID(s): 1604 (species)

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General

@ref: 39064

BacDive-ID: 138087

keywords: Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lactobacillus amylovorus CIP 104619 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from Cattle waste-corn silage.

NCBI tax id

  • NCBI tax id: 1604
  • Matching level: species

strain history

  • @ref: 39064
  • history: CIP <- 1995, ATCC <- L.K. Nakamura, USA: strain NRRL B-4549 <- G. Hrubant

doi: 10.13145/bacdive138087.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus amylovorus
  • full scientific name: Lactobacillus amylovorus Nakamura 1981

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus sobrius

@ref: 39064

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus amylovorus

type strain: no

Morphology

cell morphology

  • @ref: 39064
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39064MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
39064CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
39064positivegrowth37
53700positivegrowth37
39064positivegrowth37-45
39064nogrowth5
39064nogrowth15

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53700anaerobe
53700microaerophile
39064facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838116199urea-hydrolysis
6838116443D-tagatose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
683816731melezitose-builds acid from
6838128053melibiose-builds acid from
6838117306maltose+builds acid from
6838127941pullulan-builds acid from
6838128087glycogen-builds acid from
68381606565hippurate-hydrolysis
6838140585alpha-cyclodextrin-builds acid from
6838118333D-arabitol-builds acid from
6838130849L-arabinose-builds acid from
6838117992sucrose-builds acid from
6838116634raffinose-builds acid from
6838127082trehalose+builds acid from
6838117716lactose-builds acid from
6838130911sorbitol-builds acid from
6838116899D-mannitol-builds acid from
6838116988D-ribose-builds acid from
6838129016arginine-hydrolysis
6838029985L-glutamate-degradation
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6837928087glycogen-fermentation
6837917716lactose-fermentation
6837917306maltose+fermentation
6837916899D-mannitol-fermentation
6837916988D-ribose-fermentation
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
683794853esculin+hydrolysis
6837916199urea-hydrolysis
3906417632nitrate-reduction
3906416301nitrite-reduction
3906417632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837159640N-acetylglucosamine+builds acid from
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838029016arginine-hydrolysis
6838016199urea-hydrolysis
6837917992sucrose-fermentation
6837965327D-xylose-fermentation
6837917632nitrate-reduction
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837128053melibiose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6838115688acetoinno
6838035581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin-
6838035581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382valine arylamidase-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381beta-galactosidase+3.2.1.23
68381pyrrolidonyl arylamidase-3.4.19.3
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
39064oxidase-
39064alcohol dehydrogenase-1.1.1.1
39064amylase+
39064catalase-1.11.1.6
39064lysine decarboxylase-4.1.1.18
39064ornithine decarboxylase-4.1.1.17
68380serine arylamidase+
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380alpha-fucosidase-3.2.1.51
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68379urease-3.5.1.5
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68381beta-mannosidase-3.2.1.25
68381alpha-galactosidase-3.2.1.22
68382esterase (C 4)-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380glutamate decarboxylase-4.1.1.15
68380alpha-arabinosidase-3.2.1.55
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379catalase-1.11.1.6
68379gelatinase-
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
53700-+---+--+---+---+----

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
53700-----+----++-+-++---
39064-+--------++---+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
53700----------++++-------++--++++--++---++-+----------
39064---------++++/---------++/-+/-+++++/--+/-+---++-++/----------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
53700---+-+----+------++-++-++-+-+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
53700-++-------+------++------+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
39064---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
53700Cattle waste-corn silage
39064Cattle wastecorn silage

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Animal feed
#Host#Mammals#Bovinae (Cow, Cattle)
#Engineered#Agriculture#Livestock (Husbandry)

Safety information

risk assessment

  • @ref: 39064
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 39064

culture collection no.: CIP 104619, ATCC 33621, CCUG 37501, NRRL B-4549, LMG 13135

straininfo link

  • @ref: 94883
  • straininfo: 7336

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology8567489Cultural conditions for production of glucoamylase from Lactobacillus amylovorus ATCC 33621.James JA, Lee BHJ Appl Bacteriol10.1111/j.1365-2672.1995.tb03169.x1995Culture Media/pharmacology, Dextrins/pharmacology, Glucan 1,4-alpha-Glucosidase/antagonists & inhibitors/*biosynthesis/metabolism, Glucose/pharmacology, Hydrogen-Ion Concentration, Lactobacillus/*enzymology/growth & development, Microbiological Techniques, TemperatureCultivation
Enzymology9009073Purification of glucoamylase from Lactobacillus amylovorus ATCC 33621.James JA, Berger JL, Lee BHCurr Microbiol10.1007/s0028499001661997Amino Acid Sequence, Glucan 1,4-alpha-Glucosidase/chemistry/*isolation & purification/metabolism, Hydrogen-Ion Concentration, Lactobacillus/*enzymology, Molecular Sequence Data, Molecular Weight, TemperatureGenetics
Biotechnology34194417Efficient Secretion and Recombinant Production of a Lactobacillal alpha-amylase in Lactiplantibacillus plantarum WCFS1: Analysis and Comparison of the Secretion Using Different Signal Peptides.Tran AM, Unban K, Kanpiengjai A, Khanongnuch C, Mathiesen G, Haltrich D, Nguyen THFront Microbiol10.3389/fmicb.2021.6894132021

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
39064Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104619Collection of Institut Pasteur (CIP 104619)
53700Curators of the CCUGhttps://www.ccug.se/strain?id=37501Culture Collection University of Gothenburg (CCUG) (CCUG 37501)
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
94883Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID7336.1