Strain identifier

BacDive ID: 13794

Type strain: Yes

Species: Sulfitobacter brevis

Strain Designation: EL-162

Strain history: <- DSM <- M Labrenz <- P Hirsch, Inst. Allg. Mikrobiol. Univ. Kiel.

NCBI tax ID(s): 74348 (species)

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General

@ref: 4369

BacDive-ID: 13794

DSM-Number: 11443

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative

description: Sulfitobacter brevis EL-162 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from water.

NCBI tax id

  • NCBI tax id: 74348
  • Matching level: species

strain history

@refhistory
4369<- M. Labrenz <- P. Hirsch, Inst. Allg. Mikrobiol. Univ. Kiel
67770IAM 15345 <-- DSM 11443 <-- M. Labrenz EL-162 <-- P. Hirsch.
67771<- DSM <- M Labrenz <- P Hirsch, Inst. Allg. Mikrobiol. Univ. Kiel.

doi: 10.13145/bacdive13794.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Sulfitobacter
  • species: Sulfitobacter brevis
  • full scientific name: Sulfitobacter brevis Labrenz et al. 2000

@ref: 4369

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Sulfitobacter

species: Sulfitobacter brevis

full scientific name: Sulfitobacter brevis Labrenz et al. 2000

strain designation: EL-162

type strain: yes

Morphology

cell morphology

@refgram stainconfidence
67771negative
69480negative99.993

colony morphology

  • @ref: 4369
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 4369
  • name: EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/621a
  • composition: Name: EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a) Composition: NaCl 22.6553 g/l Agar 15.0 g/l MgCl2 x 6 H2O 4.80666 g/l Na2SO4 3.77991 g/l CaCl2 1.06343 g/l KCl 0.64076 g/l MgSO4 x 7 H2O 0.625264 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.210526 g/l NaHCO3 0.18528 g/l CaCl2 x 2 H2O 0.0703158 g/l H3BO3 0.02509 g/l SrCl2 0.02316 g/l KBr 0.00579 g/l NaF 0.002895 g/l FeSO4 x 7 H2O 0.00208422 g/l Na2MoO4 x 2 H2O 0.000266736 g/l Pyridoxine hydrochloride 0.0001 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Metall salt sol. 44 Distilled water

culture temp

@refgrowthtypetemperaturerange
4369positivegrowth20psychrophilic
67770positivegrowth20psychrophilic
67771positivegrowth20psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4369catalase+1.11.1.6
4369cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4369--------+--+----+++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
4369waterEkho Lake, Vestfold HillsAustralia and Oceania
67771From Water; East Antarctica, Ekho LakeVestfold HillsAustralia and OceaniaAntarcticaATA
67770Water from a depth of 8 m, Ekho LakeVestfold HillsAntarcticaAntarcticaATA

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

  • @ref: 4369
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sulfitobacter brevis strain DSM11443 16S ribosomal RNA gene, partial sequenceDQ9156331308ena74348
4369Sulfitobacter brevis 16S rRNA gene (strain Ekho Lake-162)Y164251342ena74348

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sulfitobacter brevis strain DSM 1144374348.5wgspatric74348
66792Sulfitobacter brevis DSM 114432619619004draftimg74348
67770Sulfitobacter brevis DSM 11443GCA_900112755contigncbi74348

GC content

@refGC-contentmethod
436957.9-58.1high performance liquid chromatography (HPLC)
6777058high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes58.131no
flagellatedno92.287no
gram-positiveno97.528no
anaerobicno97.731yes
aerobicyes89.922no
halophileyes75.864no
spore-formingno96.153no
glucose-utilyes73.886yes
thermophileno98.551yes
glucose-fermentno93.599yes

External links

@ref: 4369

culture collection no.: DSM 11443, ATCC BAA 4, KCTC 32186, JCM 21790, IAM 15345

straininfo link

  • @ref: 82972
  • straininfo: 46984

literature

  • topic: Phylogeny
  • Pubmed-ID: 10826817
  • title: Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., alpha-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake.
  • authors: Labrenz M, Tindall BJ, Lawson PA, Collins MD, Schumann P, Hirsch P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-1-303
  • year: 2000
  • mesh: Alphaproteobacteria/chemistry/*classification/cytology/physiology, Antarctic Regions, Base Composition, Culture Media, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride, Sulfites/metabolism, Temperature, *Water Microbiology
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4369Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11443)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11443
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82972Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46984.1StrainInfo: A central database for resolving microbial strain identifiers