Strain identifier

BacDive ID: 137712

Type strain: No

Species: Escherichia coli

Strain Designation: RM42B

Strain history: CIP <- 1999, T.S. Whittam, Pennsylvania Ste Univ., PA, USA: strain ECOR 35 <- H. Ochman and R.K. Selander: strain RM42B

NCBI tax ID(s): 562 (species)

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General

@ref: 38604

BacDive-ID: 137712

keywords: Bacteria, facultative anaerobe, Gram-negative, motile, rod-shaped

description: Escherichia coli RM42B is a facultative anaerobe, Gram-negative, motile bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 38604
  • history: CIP <- 1999, T.S. Whittam, Pennsylvania Ste Univ., PA, USA: strain ECOR 35 <- H. Ochman and R.K. Selander: strain RM42B

doi: 10.13145/bacdive137712.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 38604

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

strain designation: RM42B

type strain: no

Morphology

cell morphology

  • @ref: 38604
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 38604
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38604MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
38604CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
38604positivegrowth30
38604positivegrowth10-41
38604nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 38604
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol+builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
38604mannitol+fermentation29864
38604citrate-carbon source16947
38604esculin+hydrolysis4853
38604glucose+fermentation17234
38604lactose+fermentation17716
38604nitrate+reduction17632
38604nitrite+reduction16301
38604malonate-assimilation15792
38604sodium thiosulfate-builds gas from132112
38604glucose+degradation17234

antibiotic resistance

  • @ref: 38604
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 38604
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3860415688acetoin-
3860417234glucose+

enzymes

@refvalueactivityec
38604oxidase-
38604beta-galactosidase+3.2.1.23
38604alcohol dehydrogenase-1.1.1.1
38604gelatinase-
38604catalase+1.11.1.6
38604lysine decarboxylase+4.1.1.18
38604ornithine decarboxylase-4.1.1.17
38604phenylalanine ammonia-lyase-4.3.1.24
38604tryptophan deaminase-
38604urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38604-+---++-+-++++-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
38604+-+/-+++---+++++++-++--+-+/-+/-+/-+/-+++++--+---+/---+-+--+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
38604+++++++++++++++--+++++-++----+++-+--+------++--+++----+++--+++---++-++--+--+---++-++--+-+++++++-+-+

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
38604IowaUSAUSANorth America
38604IowaUnited States of AmericaUSANorth AmericaHuman, Healthy

Safety information

risk assessment

  • @ref: 38604
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 38604

culture collection no.: CIP 106054, ECOR 35

straininfo link

  • @ref: 94545
  • straininfo: 362026

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38604Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106054Collection of Institut Pasteur (CIP 106054)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
94545Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID362026.1