Strain identifier

BacDive ID: 137701

Type strain: Yes

Species: Brachyspira intermedia

Strain Designation: PWS/A

Strain history: CIP <- 1999, T. Stanton, NADC, Iowa, USA: strain PWS/A <- D. Hampson <- D. Taylor

NCBI tax ID(s): 1045858 (strain), 84377 (species)

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General

@ref: 38585

BacDive-ID: 137701

keywords: genome sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Brachyspira intermedia PWS/A is an anaerobe, mesophilic, Gram-negative bacterium of the family Brachyspiraceae.

NCBI tax id

NCBI tax idMatching level
84377species
1045858strain

strain history

  • @ref: 38585
  • history: CIP <- 1999, T. Stanton, NADC, Iowa, USA: strain PWS/A <- D. Hampson <- D. Taylor

doi: 10.13145/bacdive137701.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Brachyspirales
  • family: Brachyspiraceae
  • genus: Brachyspira
  • species: Brachyspira intermedia
  • full scientific name: Brachyspira intermedia (Stanton et al. 1997) Hampson and La 2006

  • synonyms

    @refsynonym
    20215Serpulina intermedius
    20215Serpulina intermedia

@ref: 38585

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Brachyspirales

family: Brachyspiraceae

genus: Brachyspira

species: Brachyspira intermedia

strain designation: PWS/A

type strain: yes

Morphology

cell morphology

  • @ref: 38585
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 38585

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38585MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
38585CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
38585CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 38585
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 38585
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
385854853esculin-hydrolysis
38585606565hippurate+hydrolysis
3858517632nitrate-reduction
3858516301nitrite-reduction

metabolite production

  • @ref: 38585
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
38585oxidase-
38585beta-galactosidase-3.2.1.23
38585gelatinase-
38585amylase-
38585DNase-
38585caseinase-3.4.21.50
38585catalase+1.11.1.6
38585lecithinase-
38585lipase-
38585protease-
38585urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38585-+++-+----++-+-++---

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typeisolation date
38585United KingdomGBREurope
38585United KingdomGBREuropeAnimal, Swine, intestinal contents1975

Safety information

risk assessment

  • @ref: 38585
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Brachyspira intermedia PWS/A
  • accession: 1045858.9
  • assembly level: plasmid
  • database: patric
  • NCBI tax ID: 1045858

External links

@ref: 38585

culture collection no.: CIP 105833, ATCC 51140

straininfo link

  • @ref: 94534
  • straininfo: 45269

literature

  • topic: Phylogeny
  • Pubmed-ID: 16627646
  • title: Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira intermedia comb. nov. and Brachyspira murdochii comb. nov.
  • authors: Hampson DJ, La T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64004-0
  • year: 2006
  • mesh: Base Composition, Brachyspira/*classification/enzymology/genetics/physiology, DNA, Bacterial/chemistry/genetics, Genes, rRNA, Phenotype, Phylogeny, Spirochaetales/*classification/enzymology/genetics/physiology
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38585Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105833Collection of Institut Pasteur (CIP 105833)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
94534Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID45269.1