Strain identifier

BacDive ID: 13766

Type strain: Yes

Species: Roseovarius tolerans

Strain Designation: EL-172

Strain history: CIP <- 2000, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-172

NCBI tax ID(s): 74031 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4374

BacDive-ID: 13766

DSM-Number: 11457

keywords: genome sequence, 16S sequence, Bacteria, psychrophilic, Gram-negative

description: Roseovarius tolerans EL-172 is a psychrophilic, Gram-negative bacterium that was isolated from water.

NCBI tax id

  • NCBI tax id: 74031
  • Matching level: species

strain history

@refhistory
4374<- M. Labrenz and P. Hirsch, Inst. Allg. Mikrobiol. Univ. Kiel; EL-172
67770IAM 14840 <-- DSM 11457 <-- M. Labrenz and P. Hirsch EL-172.
123559CIP <- 2000, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-172

doi: 10.13145/bacdive13766.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Roseovarius
  • species: Roseovarius tolerans
  • full scientific name: Roseovarius tolerans Labrenz et al. 1999

@ref: 4374

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Roseovarius

species: Roseovarius tolerans

full scientific name: Roseovarius tolerans Labrenz et al. 1999

strain designation: EL-172

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
123559negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4374EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a)yeshttps://mediadive.dsmz.de/medium/621aName: EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a) Composition: NaCl 22.6553 g/l Agar 15.0 g/l MgCl2 x 6 H2O 4.80666 g/l Na2SO4 3.77991 g/l CaCl2 1.06343 g/l KCl 0.64076 g/l MgSO4 x 7 H2O 0.625264 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.210526 g/l NaHCO3 0.18528 g/l CaCl2 x 2 H2O 0.0703158 g/l H3BO3 0.02509 g/l SrCl2 0.02316 g/l KBr 0.00579 g/l NaF 0.002895 g/l FeSO4 x 7 H2O 0.00208422 g/l Na2MoO4 x 2 H2O 0.000266736 g/l Pyridoxine hydrochloride 0.0001 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Metall salt sol. 44 Distilled water
39510MEDIUM 168 - for Cytophaga latercula and Flammeovirga apricayesAgar (15.000 g);Yeast extract (0.500 g);Sodium acetate (0.200 g);Tryptone (2.000 g);Beef extract (0.500 g);Synthetic sea solution - M0216 (1000.000 ml)
123559CIP Medium 168yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=168

culture temp

@refgrowthtypetemperaturerange
4374positivegrowth20psychrophilic
39510positivegrowth22psychrophilic
67770positivegrowth20psychrophilic
123559positivegrowth10-41
123559nogrowth45thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no98
69480no99.999

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12355917632nitrate-builds gas from
12355917632nitrate-reduction
12355916301nitrite-builds gas from
12355916301nitrite-reduction

antibiotic resistance

  • @ref: 123559
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123559
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 123559
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
123559oxidase+
123559beta-galactosidase-3.2.1.23
123559alcohol dehydrogenase-1.1.1.1
123559gelatinase-
123559catalase+1.11.1.6
123559gamma-glutamyltransferase-2.3.2.2
123559lysine decarboxylase-4.1.1.18
123559ornithine decarboxylase-4.1.1.17
123559tryptophan deaminase-
123559urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123559--++-++----+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
4374waterEkho LakeAustralia and Oceania
67770Water from a depth of 10 m, Ekho LakeVestfold HillsAntarcticaAntarcticaATA
123559Environment, Water samplesEkho lakeAntarcticaAntarcticaATA

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_7102.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_3328;97_4084;98_5222;99_7102&stattab=map
  • Last taxonomy: Roseovarius tolerans subclade
  • 16S sequence: Y11551
  • Sequence Identity:
  • Total samples: 84
  • soil counts: 6
  • aquatic counts: 77
  • animal counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43741Risk group (German classification)
1235591Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4374
  • description: Roseovarius tolerans 16S rRNA gene, strain Ekho Lake-172
  • accession: Y11551
  • length: 1418
  • database: ena
  • NCBI tax ID: 74031

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Roseovarius tolerans strain DSM 1145774031.7wgspatric74031
66792Roseovarius tolerans DSM 114572619619026draftimg74031
67770Roseovarius tolerans DSM 11457GCA_900109855scaffoldncbi74031

GC content

@refGC-contentmethod
437463.3-63.4high performance liquid chromatography (HPLC)
6777062.2-63.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
motileno81.026no
flagellatedno95.628no
gram-positiveno97.663no
anaerobicno97.524no
aerobicyes90.456no
halophileyes86.332no
spore-formingno97.194no
thermophileno96.869yes
glucose-utilno64.856no
glucose-fermentno92.41no

External links

@ref: 4374

culture collection no.: DSM 11457, JCM 21346, CIP 106398, IAM 14840, IFO 16695, NBRC 16695, CECT 7738

straininfo link

  • @ref: 82945
  • straininfo: 49115

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10028255Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake.Labrenz M, Collins MD, Lawson PA, Tindall BJ, Schumann P, Hirsch PInt J Syst Bacteriol10.1099/00207713-49-1-1371999Bacteriochlorophylls/*biosynthesis, Base Sequence, DNA, Bacterial/analysis, Gram-Negative Aerobic Bacteria/*classification/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/chemistry, *Water MicrobiologyGenetics
Phylogeny22941298Roseovarius sediminilitoris sp. nov., isolated from seashore sediment.Park S, Yoon JHInt J Syst Evol Microbiol10.1099/ijs.0.043737-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analysisGenetics
Phylogeny23828179Roseovarius marisflavi sp. nov., isolated from an amphioxus breeding zone in the coastal region of the Yellow Sea, China.Li Z, Zhao R, Ji S, Shi X, Zhang XHAntonie Van Leeuwenhoek10.1007/s10482-013-9965-62013Aerobiosis, Animals, Bacterial Proteins/analysis, Base Composition, Catalase/analysis, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Hydroxybutyrates/analysis, Lancelets/*growth & development, Molecular Sequence Data, Oxidoreductases/analysis, Phospholipids/analysis, Phylogeny, Polyesters/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/*isolation & purification/physiology, Seawater/*microbiology, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureGenetics
Phylogeny24384627Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores.Rajasabapathy R, Mohandass C, Dastager SG, Liu Q, Khieu TN, Son CK, Li WJ, Colaco AAntonie Van Leeuwenhoek10.1007/s10482-013-0109-92014Azores, Fatty Acids/chemistry, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/chemistry/*classification/genetics, Seawater/*microbiology, *Water MicrobiologyGenetics
Phylogeny24844264Youngimonas vesicularis gen. nov., sp. nov., of the family Rhodobacteraceae, isolated from surface seawater, reclassification of Donghicola xiamenensis Tan et al. 2009 as Pseudodonghicola xiamenensis gen. nov., comb. nov. and emended description of the genus Donghicola Yoon et al. 2007.Hameed A, Shahina M, Lin SY, Nakayan P, Liu YC, Lai WA, Hsu YHInt J Syst Evol Microbiol10.1099/ijs.0.060962-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Taiwan, Ubiquinone/chemistryGenetics
Phylogeny25209747Extremely 'vanadiphilic' multiply metal-resistant and halophilic aerobic anoxygenic phototrophs, strains EG13 and EG8, from hypersaline springs in Canada.Csotonyi JT, Maltman C, Swiderski J, Stackebrandt E, Yurkov VExtremophiles10.1007/s00792-014-0693-22014Aerobiosis, Bacteria/*classification/genetics/isolation & purification, Bacteriochlorophylls/chemistry, Biomass, Canada, DNA, Bacterial/genetics, Hydrogen-Ion Concentration, Metals/chemistry, Natural Springs/*microbiology, Photosynthesis, Phototropism, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Temperature, Vanadium/chemistry, *Water MicrobiologyTranscriptome
Phylogeny26066711Pseudoroseovarius zhejiangensis gen. nov., sp. nov., a novel alpha-proteobacterium isolated from the chemical wastewater, and reclassification of Roseovarius crassostreae as Pseudoroseovarius crassostreae comb. nov., Roseovarius sediminilitoris as Pseudoroseovarius sediminilitoris comb. nov. and Roseovarius halocynthiae as Pseudoroseovarius halocynthiae comb. nov.Sun C, Pan J, Zhang XQ, Su Y, Wu MAntonie Van Leeuwenhoek10.1007/s10482-015-0480-92015Aerobiosis, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/*isolation & purification/physiology, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Waste Water/*microbiologyGenetics
Phylogeny26446977Roseovarius algicolus sp. nov., isolated from culture fluid of Cochlidinium polykrikoides.Moon SB, Lee SSAntonie Van Leeuwenhoek10.1007/s10482-015-0600-62015Aerobiosis, Bacterial Typing Techniques, Base Composition, Catalase/analysis, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Dinoflagellida/*microbiology, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/analysis, Phospholipids/analysis, Phylogeny, Pigments, Biological/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/*isolation & purification/physiology, Sequence Analysis, DNA, TemperatureGenetics
Phylogeny29461183Roseovarius nitratireducens sp. nov., a halotolerant bacterium isolated from a saline lake.Shen X, Zhao Z, Wu C, Yu XY, Li Y, Sun C, Wu MInt J Syst Evol Microbiol10.1099/ijsem.0.0026512018
Phylogeny29664362Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain.Castro DJ, Cerezo I, Sampedro I, Martinez-Checa FInt J Syst Evol Microbiol10.1099/ijsem.0.0027442018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, *Salinity, Sequence Analysis, DNA, Soil, *Soil Microbiology, Spain, Ubiquinone/chemistryTranscriptome
Phylogeny30353463Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau.Lu HB, Xue XF, Phurbu D, Xing P, Wu QLJ Microbiol10.1007/s12275-018-8178-02018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial/genetics, Lakes/chemistry/*microbiology, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/*isolation & purification/physiology, Sequence Analysis, DNA, Species Specificity, Tibet, Ubiquinone, *Water MicrobiologyTranscriptome
Biotechnology33202548Bioleaching of Gold from Sulfidic Gold Ore Concentrate and Electronic Waste by Roseovarius tolerans and Roseovarius mucosus.Kudpeng K, Bohu T, Morris C, Thiravetyan P, Kaksonen AHMicroorganisms10.3390/microorganisms81117832020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4374Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11457)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11457
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39510Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18524
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82945Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49115.1StrainInfo: A central database for resolving microbial strain identifiers
123559Curators of the CIPCollection of Institut Pasteur (CIP 106398)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106398