Strain identifier

BacDive ID: 137565

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: 632

Strain history: CIP <- 2002, CCUG <- P. Baumann, Univ. of California, Davis, USA: strain 218 Pseudomonas perfectomarinus <- CE ZoBell: strain 632

NCBI tax ID(s): 32042 (strain), 203192 (species), 316 (species)

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General

@ref: 38224

BacDive-ID: 137565

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Pseudomonas stutzeri 632 is an obligate aerobe, mesophilic, motile bacterium that was isolated from Marine source.

NCBI tax id

NCBI tax idMatching level
32042strain
203192species
316species

strain history

@refhistory
67770IAM 12931 <-- ATCC 14405 <-- C. E. ZoBell 632.
38224CIP <- 2002, CCUG <- P. Baumann, Univ. of California, Davis, USA: strain 218 Pseudomonas perfectomarinus <- CE ZoBell: strain 632

doi: 10.13145/bacdive137565.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 38224

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

strain designation: 632

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.707
6948099.999negative
38224yesnegativerod-shaped

pigmentation

  • @ref: 38224
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38224MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
38224CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
38224positivegrowth25mesophilic
46451positivegrowth25mesophilic
67770positivegrowth25mesophilic
38224positivegrowth10-37
38224nogrowth5psychrophilic
38224nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 38224
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.965

halophily

  • @ref: 38224
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3822416947citrate+carbon source
382244853esculin-hydrolysis
3822417632nitrate+reduction
3822416301nitrite+reduction
3822417632nitrate-respiration

antibiotic resistance

  • @ref: 38224
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 38224
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
38224oxidase+
38224beta-galactosidase-3.2.1.23
38224alcohol dehydrogenase-1.1.1.1
38224gelatinase-
38224amylase+
38224DNase-
38224caseinase-3.4.21.50
38224catalase+1.11.1.6
38224tween esterase+
38224lecithinase-
38224lipase-
38224lysine decarboxylase-4.1.1.18
38224ornithine decarboxylase-4.1.1.17
38224protease+
38224tryptophan deaminase-
38224urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38224-+++-++-+--+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
38224++-------++--------------------+-----------++----++--+++----+--------------++--++++-+--+++++++---++

Isolation, sampling and environmental information

isolation

  • @ref: 38224
  • sample type: Marine source

Safety information

risk assessment

  • @ref: 38224
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Stutzerimonas stutzeri ATCC 14405GCA_015291885completencbi316
66792Stutzerimonas stutzeri ATCC 14405 = CCUG 16156GCA_000237885contigncbi32042
66792Pseudomonas stutzeri ATCC 14405 = CCUG 1615632042.3wgspatric32042
66792Pseudomonas stutzeri strain ATCC 14405316.569completepatric316
66792Pseudomonas stutzeri ZoBell 632, ATCC 144052529293194draftimg32042

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes89.921no
flagellatedyes90.341no
gram-positiveno98.775no
anaerobicno97.021no
aerobicyes91.175no
halophileno84.512no
spore-formingno94.005no
thermophileno99.821yes
glucose-utilyes90.869no
glucose-fermentno92.257no

External links

@ref: 38224

culture collection no.: CIP 107696, CCUG 16156, ATCC 14405, LMG 2243, NCIMB 568, JCM 20778, IAM 12931

straininfo link

  • @ref: 94430
  • straininfo: 4333

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1644760NosR, a membrane-bound regulatory component necessary for expression of nitrous oxide reductase in denitrifying Pseudomonas stutzeri.Cuypers H, Viebrock-Sambale A, Zumft WGJ Bacteriol10.1128/jb.174.16.5332-5339.19921992Amino Acid Sequence, Bacterial Proteins/genetics/*metabolism, Base Sequence, Blotting, Northern, Blotting, Western, Cell Membrane/enzymology, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, *Genes, Regulator, Genetic Complementation Test, Membrane Proteins/genetics/*metabolism, Molecular Sequence Data, Multigene Family, Oxidoreductases/*genetics/metabolism, Plasmids, Pseudomonas/*enzymology/geneticsMetabolism
Metabolism6329753NMR studies of a dihaem cytochrome from Pseudomonas perfectomarinus (ATCC 14405).Moura I, Liu MC, LeGall J, Peck HD Jr, Payne WJ, Xavier AV, Moura JJEur J Biochem10.1111/j.1432-1033.1984.tb08191.x1984Chemical Phenomena, Chemistry, Cytochrome c Group/*metabolism, Heme, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Models, Chemical, Oxidation-Reduction, Pseudomonas/*enzymology, TemperatureEnzymology
Metabolism6833178Comparison of cytochromes from anaerobically and aerobically grown cells of Pseudomonas perfectomarinus.Liu MC, Payne WJ, Peck HD Jr, LeGall JJ Bacteriol10.1128/jb.154.1.278-286.19831983Aerobiosis, Anaerobiosis, Cell Membrane/metabolism, Cytochromes/*analysis, Pseudomonas/*metabolism, Spectrophotometry
Genetics7811935Solution conformation of cytochrome c-551 from Pseudomonas stutzeri ZoBell determined by NMR.Cai M, Timkovich RBiophys J10.1016/S0006-3495(94)80590-91994Amino Acid Sequence, *Bacterial Proteins, Biophysical Phenomena, Biophysics, Cytochrome c Group/*chemistry/genetics, Magnetic Resonance Spectroscopy, Models, Molecular, Molecular Sequence Data, Molecular Structure, Protein Conformation, Protein Folding, Pseudomonas/*chemistry/genetics, Solutions, ThermodynamicsProteome
Enzymology8245846Antibody and DNA probes for detection of nitrite reductase in seawater.Ward BB, Cockcroft AR, Kilpatrick KAJ Gen Microbiol10.1099/00221287-139-9-22851993Antibodies, Bacterial, Base Sequence, DNA Probes/genetics, DNA, Bacterial/analysis/genetics, Genes, Bacterial, Molecular Sequence Data, Nitrite Reductases/*analysis/genetics/immunology, Pseudomonas/*enzymology/genetics/isolation & purification, Seawater/*analysis, Species Specificity, *Water MicrobiologyPhylogeny
Phylogeny857349616S rRNA gene sequence analysis relative to genomovars of Pseudomonas stutzeri and proposal of Pseudomonas balearica sp. nov.Bennasar A, Rossello-Mora R, Lalucat J, Moore ERInt J Syst Bacteriol10.1099/00207713-46-1-2001996Base Sequence, DNA, Bacterial/genetics, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, Pseudomonas/*classification/genetics, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*geneticsGenetics
Genetics8950369Sequence analysis of an internal 9.72-kb segment from the 30-kb denitrification gene cluster of Pseudomonas stutzeri.Glockner AB, Zumft WGBiochim Biophys Acta10.1016/s0005-2728(96)00108-91996Amino Acid Sequence, *Genes, Bacterial, Molecular Sequence Data, *Multigene Family, Nitrogen Fixation/genetics, Open Reading Frames, Pseudomonas/*genetics, Restriction MappingTranscriptome
Metabolism10368138Nitrate and nitrite control of respiratory nitrate reduction in denitrifying Pseudomonas stutzeri by a two-component regulatory system homologous to NarXL of Escherichia coli.Hartig E, Schiek U, Vollack KU, Zumft WGJ Bacteriol10.1128/JB.181.12.3658-3665.19991999Amino Acid Sequence, *Anion Transport Proteins, Bacterial Proteins/chemistry/*genetics/*metabolism, Base Sequence, Carrier Proteins/genetics/*metabolism, Cloning, Molecular, DNA-Binding Proteins/chemistry/*genetics/metabolism, Escherichia coli/*metabolism, *Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Nitrate Reductase, Nitrate Reductases/genetics/*metabolism, Nitrate Transporters, Nitrates/*metabolism, Nitrites/*metabolism, Oxidation-Reduction, Oxygen Consumption, Protein Kinases/chemistry/*genetics/metabolism, Pseudomonas/*genetics/*metabolism, Recombinant Proteins/metabolism, Restriction Mapping, Sequence Alignment, Sequence Homology, Amino Acid, Transcription, Genetic, Transcriptional ActivationEnzymology
Pathogenicity10540232Comparative responses of Pseudomonas stutzeri and Pseudomonas aeruginosa to antibacterial agents.Tattawasart U, Maillard JY, Furr JR, Russell ADJ Appl Microbiol10.1046/j.1365-2672.1999.00811.x1999Anti-Bacterial Agents/*pharmacology, Microbial Sensitivity Tests, Pseudomonas/*drug effects, Pseudomonas aeruginosa/*drug effects, Species SpecificityPhylogeny
Enzymology10691974Structural investigations of the CuA centre of nitrous oxide reductase from Pseudomonas stutzeri by site-directed mutagenesis and X-ray absorption spectroscopy.Charnock JM, Dreusch A, Korner H, Neese F, Nelson J, Kannt A, Michel H, Garner CD, Kroneck PM, Zumft WGEur J Biochem10.1046/j.1432-1327.2000.01131.x2000Amino Acid Sequence, Base Sequence, Binding Sites, Copper/*chemistry, DNA Primers, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Oxidoreductases/*chemistry/genetics, Protein Conformation, Pseudomonas/*enzymology, Sequence Homology, Amino Acid, Spectrum AnalysisGenetics
Metabolism15743947Functional domains of NosR, a novel transmembrane iron-sulfur flavoprotein necessary for nitrous oxide respiration.Wunsch P, Zumft WGJ Bacteriol10.1128/JB.187.6.1992-2001.20052005Amino Acid Sequence, Bacterial Proteins/*chemistry/genetics/*metabolism, Catalytic Domain, Energy Metabolism, Flavoproteins/chemistry/genetics/metabolism, Gene Expression Regulation, Bacterial, Iron-Sulfur Proteins/chemistry/genetics/metabolism, Membrane Proteins/*chemistry/genetics/*metabolism, Molecular Sequence Data, Mutagenesis, Nitrous Oxide/*metabolism, Protein Structure, Tertiary, Pseudomonas stutzeri/genetics/*metabolism, Recombinant Proteins/chemistry/genetics/metabolismGenetics
Genetics22328767Draft genome of Pseudomonas stutzeri strain ZoBell (CCUG 16156), a marine isolate and model organism for denitrification studies.Pena A, Busquets A, Gomila M, Bosch R, Nogales B, Garcia-Valdes E, Lalucat J, Bennasar AJ Bacteriol10.1128/JB.06648-112012DNA, Bacterial/*chemistry/*genetics, Denitrification, *Genome, Bacterial, Molecular Sequence Data, Pseudomonas stutzeri/*genetics/*isolation & purification/metabolism, Seawater/*microbiology, Sequence Analysis, DNATranscriptome
24189920Immunofluorescence detection of the denitrifying strain Pseudomonas stutzeri (ATCC 14405) in seawater and intertidal sediment environments.Ward BB, Cockcroft ARMicrob Ecol10.1007/BF001718901993
Metabolism26905325Enhanced denitrification of Pseudomonas stutzeri by a bioelectrochemical system assisted with solid-phase humin.Xiao Z, Awata T, Zhang D, Zhang C, Li Z, Katayama AJ Biosci Bioeng10.1016/j.jbiosc.2015.11.0042016*Denitrification, Electricity, Electrochemical Techniques, Electrodes, Electron Transport, Electrons, *Humic Substances, Nitrates/chemistry/metabolism, Nitrogen/chemistry/metabolism, Oxidation-Reduction, Pseudomonas stutzeri/*metabolism
Metabolism26975755Denitrification by Pseudomonas stutzeri coupled with CO2 reduction by Sporomusa ovata with hydrogen as an electron donor assisted by solid-phase humin.Xiao Z, Awata T, Zhang D, Katayama AJ Biosci Bioeng10.1016/j.jbiosc.2016.02.0022016Acetic Acid/metabolism, Ammonium Compounds/metabolism, Carbon Dioxide/*metabolism, Coculture Techniques, *Denitrification, Electron Transport, *Electrons, Heterotrophic Processes, *Humic Substances, Hydrogen/*metabolism, Nitrates/metabolism, Nitrites/metabolism, Nitrogen/metabolism, Nitrous Oxide/metabolism, Pseudomonas stutzeri/cytology/*metabolism, Veillonellaceae/cytology/*metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38224Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107696Collection of Institut Pasteur (CIP 107696)
46451Curators of the CCUGhttps://www.ccug.se/strain?id=16156Culture Collection University of Gothenburg (CCUG) (CCUG 16156)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
94430Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID4333.1