Strain identifier

BacDive ID: 137392

Type strain: No

Species: Klebsiella pneumoniae

Strain Designation: P20

Strain history: CIP <- 1995, L.S. Tzouvelekis, Hellenic Pasteur Inst., Athens, Greece: strain P20

NCBI tax ID(s): 573 (species)

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General

@ref: 37670

BacDive-ID: 137392

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Klebsiella pneumoniae P20 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 573
  • Matching level: species

strain history

@refhistory
376701995, L.S. Tzouvelekis, Inst. Pasteur, Athens, Greece: strain P20
37670CIP <- 1995, L.S. Tzouvelekis, Hellenic Pasteur Inst., Athens, Greece: strain P20

doi: 10.13145/bacdive137392.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae
  • full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Hyalococcus pneumoniae

@ref: 37670

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae

strain designation: P20

type strain: no

Morphology

cell morphology

  • @ref: 37670
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37670MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
37670CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
37670positivegrowth30mesophilic
37670positivegrowth10-41
37670nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 37670
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3767029864mannitol+fermentation
3767016947citrate+carbon source
3767017234glucose+fermentation
3767017716lactose+fermentation
3767017632nitrate+reduction
3767016301nitrite+reduction
3767015792malonate+assimilation
37670132112sodium thiosulfate-builds gas from
3767017234glucose+degradation

antibiotic resistance

  • @ref: 37670
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 37670
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3767015688acetoin+
3767017234glucose-

enzymes

@refvalueactivityec
37670oxidase-
37670beta-galactosidase+3.2.1.23
37670alcohol dehydrogenase-1.1.1.1
37670gelatinase-
37670catalase+1.11.1.6
37670lysine decarboxylase+4.1.1.18
37670ornithine decarboxylase-4.1.1.17
37670phenylalanine ammonia-lyase-4.3.1.24
37670tryptophan deaminase-
37670urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
37670-+---++---++++-++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
37670+++++++++++++++++++++++++-+-+++++++-++-----+++--++++-++++--++-+++--+-+--++-+---++--+--+-++++++++---

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
37670AthensGreeceGRCEurope
37670AthensGreeceGRCEuropeHuman, Blood1991

Safety information

risk assessment

  • @ref: 37670
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 37670

culture collection no.: CIP 104341

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
37670Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104341Collection of Institut Pasteur (CIP 104341)
68382Automatically annotated from API zym