Strain identifier
BacDive ID: 137015
Type strain: 
Species: Ensifer garamanticus
Strain history: CIP <- 2008, P. de Lajudie, Lab. Symbioses Tropicales et Méditerranéennes, UMR 113 IRD-CIRAD-SupAgro-Université Montpellier, Montpellier, France: strain ORS 1401
NCBI tax ID(s): 680247 (species)
version 8.1 (current version)
General
@ref: 36841
BacDive-ID: 137015
keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Ensifer garamanticus CIP 109848 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Rhizobiaceae.
NCBI tax id
- NCBI tax id: 680247
- Matching level: species
strain history
- @ref: 36841
- history: CIP <- 2008, P. de Lajudie, Lab. Symbioses Tropicales et Méditerranéennes, UMR 113 IRD-CIRAD-SupAgro-Université Montpellier, Montpellier, France: strain ORS 1401
doi: 10.13145/bacdive137015.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Rhizobiaceae
- genus: Ensifer
- species: Ensifer garamanticus
- full scientific name: Ensifer garamanticus Merabet et al. 2010
synonyms
- @ref: 20215
- synonym: Sinorhizobium garamanticum
@ref: 36841
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Hyphomicrobiales
family: Rhizobiaceae
genus: Ensifer
species: Ensifer garamanticus
type strain: no
Morphology
cell morphology
- @ref: 36841
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 36841 | MEDIUM 705 - for Ensifer | yes | Distilled water make up to (1.000 l);Sodium chloride (0.050 g);Magnesiumsulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.040 g);Agar (15.000 g);Yeast extract (1.000 g);Potassium di-hydrogen phosphate (0.500 g);Sodium glutamate (0.500 g);Ferri | |
| 36841 | CIP Medium 705 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=705 |
culture temp
- @ref: 36841
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 36841
- oxygen tolerance: obligate aerobe
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 36841 | 17632 | nitrate | + | reduction |
| 36841 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 36841
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 36841 | oxidase | + | |
| 36841 | catalase | + | 1.11.1.6 |
| 36841 | urease | + | 3.5.1.5 |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | + | |
| 68382 | esterase lipase (C 8) | - | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | + | |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | trypsin | + | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 36841 | - | + | + | - | - | + | + | - | + | - | + | - | - | + | - | + | + | + | - | - |
Isolation, sampling and environmental information
isolation
| @ref | country | origin.country | continent | sample type | isolation date |
|---|---|---|---|---|---|
| 36841 | Tunisia | TUN | Africa | ||
| 36841 | Tunisia | TUN | Africa | Plant, Root nodule, Argyrolobium uniflorum | 1993 |
Safety information
risk assessment
- @ref: 36841
- biosafety level: 1
- biosafety level comment: Risk group (French classification)
External links
@ref: 36841
culture collection no.: CIP 109848, LMG 24693, ORS 1401
straininfo link
- @ref: 94003
- straininfo: 371372
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 36841 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109848 | Collection of Institut Pasteur (CIP 109848) | |
| 68382 | Automatically annotated from API zym | |||
| 94003 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID371372.1 |