Strain identifier

BacDive ID: 136832

Type strain: No

Species: Achromobacter insulatus

Strain Designation: 2254

Strain history: CIP <- 1983, Lab. Ident. Inst. Pasteur, Paris, France: strain R 931, Achromobacter xylosoxidans denitrificans <- F. Pichinoty <- Gilardi: strain 2254

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General

@ref: 36509

BacDive-ID: 136832

keywords: obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Achromobacter insulatus 2254 is an obligate aerobe, mesophilic, Gram-negative prokaryote of the family Not assigned to family.

strain history

  • @ref: 36509
  • history: CIP <- 1983, Lab. Ident. Inst. Pasteur, Paris, France: strain R 931, Achromobacter xylosoxidans denitrificans <- F. Pichinoty <- Gilardi: strain 2254

doi: 10.13145/bacdive136832.20230509.8.1

Name and taxonomic classification

@ref: 36509

phylum: Not assigned to order

class: Not assigned to order

order: Not assigned to order

family: Not assigned to family

genus: Achromobacter

species: Achromobacter insulatus

strain designation: 2254

type strain: no

Morphology

cell morphology

  • @ref: 36509
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 36509
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36509MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
36509CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
36509CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
36509positivegrowth30mesophilic
36509positivegrowth15-41
36509nogrowth5psychrophilic
36509nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 36509
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
36509NaClpositivegrowth0-6 %
36509NaClnogrowth8 %
36509NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3650916947citrate+carbon source
365094853esculin-hydrolysis
36509606565hippurate+hydrolysis
3650917632nitrate+builds gas from
3650917632nitrate+reduction
3650916301nitrite+builds gas from
3650916301nitrite+reduction
3650915792malonate-assimilation
3650917632nitrate+respiration

antibiotic resistance

  • @ref: 36509
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 36509
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3650915688acetoin-
3650917234glucose-

enzymes

@refvalueactivityec
36509oxidase+
36509beta-galactosidase-3.2.1.23
36509alcohol dehydrogenase-1.1.1.1
36509gelatinase-
36509amylase-
36509DNase-
36509caseinase-3.4.21.50
36509catalase+1.11.1.6
36509tween esterase-
36509gamma-glutamyltransferase+2.3.2.2
36509lecithinase-
36509lipase-
36509lysine decarboxylase-4.1.1.18
36509ornithine decarboxylase-4.1.1.17
36509phenylalanine ammonia-lyase-4.3.1.24
36509protease-
36509tryptophan deaminase-
36509urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
36509-++--+----+---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
36509-------------------------------------------+++-++++-++----+-+++--+-+-----+-++-++++-----++++++++-+++

Safety information

risk assessment

  • @ref: 36509
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 36509

culture collection no.: CIP 100009

Reference

@idauthorscataloguedoi/urltitle
36509Curators of the CIPCollection of Institut Pasteur (CIP 100009)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20100009
68382Automatically annotated from API zym