Strain identifier

BacDive ID: 136513

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PAO 25

Strain history: CIP <- 2000, F. Gasser, Inst. Pasteur, Paris, France <- D. Haas, Inst. Pasteur, Paris, France: strain PAO 25

NCBI tax ID(s): 287 (species)

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General

@ref: 35935

BacDive-ID: 136513

keywords: Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped

description: Pseudomonas aeruginosa PAO 25 is an obligate aerobe, Gram-negative, motile bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 35935
  • history: CIP <- 2000, F. Gasser, Inst. Pasteur, Paris, France <- D. Haas, Inst. Pasteur, Paris, France: strain PAO 25

doi: 10.13145/bacdive136513.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 35935

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

strain designation: PAO 25

type strain: no

Morphology

cell morphology

  • @ref: 35935
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 35935
  • production: yes
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35935MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
35935CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
35935positivegrowth30
35935positivegrowth5-41

Physiology and metabolism

oxygen tolerance

  • @ref: 35935
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
35935NaClpositivegrowth0-6 %
35935NaClnogrowth8 %
35935NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3593516947citrate+carbon source
359354853esculin-hydrolysis
3593517632nitrate+reduction
3593516301nitrite+reduction
3593517632nitrate+respiration

antibiotic resistance

  • @ref: 35935
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 35935
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
35935oxidase+
35935beta-galactosidase-3.2.1.23
35935alcohol dehydrogenase+1.1.1.1
35935gelatinase+
35935amylase-
35935DNase-
35935caseinase+3.4.21.50
35935catalase+1.11.1.6
35935tween esterase-
35935lecithinase+
35935lipase+
35935lysine decarboxylase-4.1.1.18
35935ornithine decarboxylase-4.1.1.17
35935protease+
35935tryptophan deaminase-
35935urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35935---++++-+-+---------

Safety information

risk assessment

  • @ref: 35935
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35935

culture collection no.: CIP 106385

straininfo link

  • @ref: 93654
  • straininfo: 69772

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35935Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106385Collection of Institut Pasteur (CIP 106385)
68382Automatically annotated from API zym
93654Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69772.1