Strain identifier
BacDive ID: 136512
Type strain:
Species: Pseudomonas aeruginosa
Strain Designation: PAO 12
Strain history: CIP <- 2000, F. Gasser, Inst. Pasteur, Paris, France <- D. Haas, Inst. Pasteur, Paris, France: strain PAO 12
NCBI tax ID(s): 287 (species)
General
@ref: 35934
BacDive-ID: 136512
keywords: Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped
description: Pseudomonas aeruginosa PAO 12 is an obligate aerobe, Gram-negative, motile bacterium of the family Pseudomonadaceae.
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
- @ref: 35934
- history: CIP <- 2000, F. Gasser, Inst. Pasteur, Paris, France <- D. Haas, Inst. Pasteur, Paris, France: strain PAO 12
doi: 10.13145/bacdive136512.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 35934
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
strain designation: PAO 12
type strain: no
Morphology
cell morphology
- @ref: 35934
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 35934
- production: yes
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
35934 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
35934 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature |
---|---|---|---|
35934 | positive | growth | 30 |
35934 | positive | growth | 10-41 |
35934 | no | growth | 5 |
Physiology and metabolism
oxygen tolerance
- @ref: 35934
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
35934 | NaCl | positive | growth | 0-2 % |
35934 | NaCl | no | growth | 6 % |
35934 | NaCl | no | growth | 8 % |
35934 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
35934 | 16947 | citrate | + | carbon source |
35934 | 4853 | esculin | - | hydrolysis |
35934 | 17632 | nitrate | + | reduction |
35934 | 16301 | nitrite | + | reduction |
35934 | 15882 | phenol | + | degradation |
35934 | 17632 | nitrate | + | respiration |
antibiotic resistance
- @ref: 35934
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 35934
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
35934 | oxidase | + | |
35934 | beta-galactosidase | - | 3.2.1.23 |
35934 | alcohol dehydrogenase | + | 1.1.1.1 |
35934 | gelatinase | + | |
35934 | amylase | - | |
35934 | DNase | - | |
35934 | caseinase | + | 3.4.21.50 |
35934 | catalase | + | 1.11.1.6 |
35934 | tween esterase | + | |
35934 | lecithinase | - | |
35934 | lipase | + | |
35934 | lysine decarboxylase | - | 4.1.1.18 |
35934 | ornithine decarboxylase | - | 4.1.1.17 |
35934 | protease | + | |
35934 | tryptophan deaminase | - | |
35934 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
35934 | - | - | - | + | + | + | - | - | + | - | + | - | - | - | - | - | - | - | - | - |
Safety information
risk assessment
- @ref: 35934
- biosafety level: 2
- biosafety level comment: Risk group (French classification)
External links
@ref: 35934
culture collection no.: CIP 106384
straininfo link
- @ref: 93653
- straininfo: 69771
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
35934 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106384 | Collection of Institut Pasteur (CIP 106384) | |
68382 | Automatically annotated from API zym | |||
93653 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID69771.1 |