Strain identifier

BacDive ID: 136501

Type strain: No

Species: Bacillus subtilis

Strain Designation: 168 CA

Strain history: CIP <- 1999, G. Rapoport, Inst. Pasteur, Paris, France: strain 168 CA <- J. Spizizen

NCBI tax ID(s): 1423 (species)

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General

@ref: 35922

BacDive-ID: 136501

keywords: Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Bacillus subtilis 168 CA is a facultative anaerobe, spore-forming, Gram-positive bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 1423
  • Matching level: species

strain history

  • @ref: 35922
  • history: CIP <- 1999, G. Rapoport, Inst. Pasteur, Paris, France: strain 168 CA <- J. Spizizen

doi: 10.13145/bacdive136501.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus subtilis
  • full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio subtilis

@ref: 35922

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus subtilis

strain designation: 168 CA

type strain: no

Morphology

cell morphology

  • @ref: 35922
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 35922
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35922MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
35922CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
35922positivegrowth30
35922positivegrowth22-37
35922nogrowth10
35922nogrowth45
35922nogrowth55

culture pH

  • @ref: 35922
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 35922
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 35922
  • spore formation: yes

halophily

  • @ref: 35922
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol+builds acid from16899
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
35922citrate-carbon source16947
35922esculin+hydrolysis4853
35922hippurate+hydrolysis606565
35922nitrate+reduction17632
35922nitrite-reduction16301
35922nitrate+respiration17632

metabolite production

  • @ref: 35922
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3592215688acetoin+
3592217234glucose-

enzymes

@refvalueactivityec
35922oxidase+
35922beta-galactosidase+3.2.1.23
35922alcohol dehydrogenase-1.1.1.1
35922gelatinase+
35922amylase+
35922DNase+
35922caseinase+3.4.21.50
35922catalase+1.11.1.6
35922tween esterase+
35922gamma-glutamyltransferase+2.3.2.2
35922lecithinase-
35922lipase-
35922lysine decarboxylase-4.1.1.18
35922ornithine decarboxylase-4.1.1.17
35922protease+
35922tryptophan deaminase-
35922urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35922-+++-------+++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35922---+/-------+++---+/-++/----+/-+++++/---+++/---+/-+-+/-+/-------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
35922++-++-+++++----++++++++--------++++++----+-++----+++-+-+---++--------------+--+++-----+--++++++----

Isolation, sampling and environmental information

isolation

  • @ref: 35922
  • isolation date: 1958

Safety information

risk assessment

  • @ref: 35922
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35922

culture collection no.: CIP 106309

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35922Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106309Collection of Institut Pasteur (CIP 106309)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym