Strain identifier

BacDive ID: 1364

Type strain: Yes

Species: Lentibacillus halodurans

Strain Designation: 8-1

Strain history: CIP <- 2007, DSMZ <- P. Zhou, Chinese Academy of Sciences, Beijing, China: strain 8-1

NCBI tax ID(s): 237679 (species)

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General

@ref: 7516

BacDive-ID: 1364

DSM-Number: 18342

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, moderately halophilic, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Lentibacillus halodurans 8-1 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from salt lake soil.

NCBI tax id

  • NCBI tax id: 237679
  • Matching level: species

strain history

@refhistory
7516<- Pei-Jin Zhou <- strain 8-1
67770CGMCC 1.3702 <-- P. Ren 8-1.
119804CIP <- 2007, DSMZ <- P. Zhou, Chinese Academy of Sciences, Beijing, China: strain 8-1

doi: 10.13145/bacdive1364.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus halodurans
  • full scientific name: Lentibacillus halodurans Yuan et al. 2007

@ref: 7516

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus halodurans

full scientific name: Lentibacillus halodurans Yuan et al. 2007

strain designation: 8-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23001positive1.5-2.5 µm0.5 µmrod-shapedno
119804positiverod-shapedyes

colony morphology

  • @ref: 23001
  • colony size: 4.0-5.0 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: HM medium with 10 % (w/v) NaCl

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7516HALOBACTERIA MEDIUM (DSMZ Medium 372)yeshttps://mediadive.dsmz.de/medium/372Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
23001HM medium with 10 % (w/v) NaClyes
42177MEDIUM 330 - for Natrinema pallidumyesDistilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
119804CIP Medium 330yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=330

culture temp

@refgrowthtypetemperature
7516positivegrowth30
23001positivegrowth22.0-45.0
23001positiveoptimum30.0
42177positivegrowth30
67770positivegrowth37

culture pH

@refabilitytypepHPH range
23001positivegrowth6.0-9.0alkaliphile
23001positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23001
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23001spherical or oval,cell-terminal,in swollen sporangiaendosporeyes
69480yes91.842
69481yes100

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23001moderately halophilicNaClpositivegrowth5.0-30.0 %(w/v)
23001NaCloptimum8.0-12.0 %

murein

  • @ref: 7516
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2300165327D-xylose-builds acid from
2300117754glycerol-builds acid from
2300117716lactose-builds acid from
2300117306maltose-builds acid from
23001casein-hydrolysis
230014853esculin-hydrolysis
2300128017starch-hydrolysis
2300153424tween 20-hydrolysis
2300153426tween 80-hydrolysis
2300117632nitrate-reduction
2300115824D-fructose+builds acid from
2300117634D-glucose+builds acid from
2300116024D-mannose+builds acid from
2300122599arabinose+carbon source
2300117057cellobiose+carbon source
2300115824D-fructose+carbon source
2300112936D-galactose+carbon source
2300117634D-glucose+carbon source
2300116024D-mannose+carbon source
2300116634raffinose+carbon source
2300117317D-sorbose+carbon source
2300116813galactitol+carbon source
2300117113erythritol+carbon source
230014853esculin+carbon source
2300117754glycerol+carbon source
2300117268myo-inositol+carbon source
2300115443inulin+carbon source
2300117266L-sorbose+carbon source
2300117716lactose+carbon source
2300117306maltose+carbon source
2300129864mannitol+carbon source
230016731melezitose+carbon source
2300128053melibiose+carbon source
2300126546rhamnose+carbon source
2300117814salicin+carbon source
2300117992sucrose+carbon source
2300127082trehalose+carbon source
2300118222xylose+carbon source
230015291gelatin+hydrolysis
2300153423tween 40+hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11980417632nitrate-reduction
11980416301nitrite-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2300128669bacitracinyesyes0.04 Unit
2300118208penicillin gyesyes10 Unit
23001100246norfloxacinyesyes10 µg
2300117076streptomycinyesyes10 µg
2300117698chloramphenicolyesyes30 µg
230016104kanamycinyesyes30 µg
230017507neomycinyesyes30 µg
2300127902tetracyclineyesyes30 µg
2300128971ampicillinyesyes10 µg
2300148923erythromycinyesyes15 µg
2300128368novobiocinyesyes30 µg
2300128001vancomycinyesyes30 µg
23001100241ciprofloxacinyesyes5 µg
2300128077rifampicinyesyes5 µg

metabolite production

  • @ref: 119804
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
23001catalase+1.11.1.6
23001cytochrome oxidase+1.9.3.1
23001urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119804oxidase+
119804catalase+1.11.1.6
119804urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119804-+---++--------+-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119804------------------------------------------------+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7516salt lake soilAi-Ging Lake, Xin-Jiang ProvinceChinaCHNAsia
67770Sediment of a neutral salt lake in Xin-JiangChinaCHNAsia
119804Environment, Salt lake soil, sediment sampleXinjiangChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_18403.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3088;97_11300;98_13897;99_18403&stattab=map
  • Last taxonomy: Lentibacillus halodurans
  • 16S sequence: AY321433
  • Sequence Identity:
  • Total samples: 6
  • soil counts: 1
  • aquatic counts: 2
  • animal counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
75161Risk group (German classification)
1198041Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7516
  • description: Lentibacillus halodurans strain 8-1 16S ribosomal RNA gene, partial sequence
  • accession: AY321433
  • length: 1466
  • database: nuccore
  • NCBI tax ID: 237679

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lentibacillus halodurans strain CGMCC 1.3702237679.4wgspatric237679
66792Lentibacillus halodurans CGMCC 1.37022617270778draftimg237679
67770Lentibacillus halodurans CGMCC 1.3702GCA_900112045scaffoldncbi237679

GC content

@refGC-contentmethod
751643.4
2300143.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes81.902yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.61yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes91.842no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes78.125no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno89.177no
69480flagellatedmotile2+Ability to perform flagellated movementyes85.121no

External links

@ref: 7516

culture collection no.: DSM 18342, CGMCC 1.3702, CIP 109588, JCM 12770

straininfo link

  • @ref: 71015
  • straininfo: 297250

literature

  • topic: Phylogeny
  • Pubmed-ID: 17329773
  • title: Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China.
  • authors: Yuan S, Ren P, Liu J, Xue Y, Ma Y, Zhou P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64547-0
  • year: 2007
  • mesh: Bacillaceae/*classification/genetics/isolation & purification/physiology, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Salts
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7516Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18342)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18342
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23001Sanqing Yuan,Peigen Ren,Jun Liu,Yanfen Xue,Yanhe Ma,Peijin Zhou10.1099/ijs.0.64547-0Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, ChinaIJSEM 57: 485-488 200717329773
42177Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7349
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71015Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297250.1StrainInfo: A central database for resolving microbial strain identifiers
119804Curators of the CIPCollection of Institut Pasteur (CIP 109588)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109588