Strain identifier

BacDive ID: 135935

Type strain: No

Species: Lysinibacillus sphaericus

Strain Designation: A20

Strain history: CIP <- 2000, L.K. Nakamura, USDA, Peoria, Illinois, USA <- N.R. Smith, USDA <- C. Lamana, Washington State College, USA: strain A20

NCBI tax ID(s): 1421 (species)

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General

@ref: 35214

BacDive-ID: 135935

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Lysinibacillus sphaericus A20 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Caryophanaceae.

NCBI tax id

  • NCBI tax id: 1421
  • Matching level: species

strain history

  • @ref: 35214
  • history: CIP <- 2000, L.K. Nakamura, USDA, Peoria, Illinois, USA <- N.R. Smith, USDA <- C. Lamana, Washington State College, USA: strain A20

doi: 10.13145/bacdive135935.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Lysinibacillus
  • species: Lysinibacillus sphaericus
  • full scientific name: Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

  • synonyms

    @refsynonym
    20215Lysinibacillus varians
    20215Bacillus sphaericus
    20215Lysinibacillus mangiferahumi
    20215Lysinibacillus mangiferihumi
    20215Lysinibacillus tabacifolii

@ref: 35214

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Lysinibacillus

species: Lysinibacillus sphaericus

strain designation: A20

type strain: no

Morphology

cell morphology

  • @ref: 35214
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 35214
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35214MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
35214CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
35214CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
35214positivegrowth30mesophilic
35214positivegrowth22-45
35214nogrowth10psychrophilic
35214nogrowth55thermophilic

culture pH

  • @ref: 35214
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 35214
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 35214
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
35214NaClpositivegrowth0-6 %
35214NaClnogrowth8 %
35214NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
35214esculin-hydrolysis4853
35214hippurate+hydrolysis606565
35214nitrate-reduction17632
35214nitrite-reduction16301
35214nitrate-respiration17632

metabolite production

  • @ref: 35214
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3521415688acetoin-
3521417234glucose-

enzymes

@refvalueactivityec
35214oxidase+
35214beta-galactosidase-3.2.1.23
35214alcohol dehydrogenase-1.1.1.1
35214gelatinase-
35214amylase-
35214DNase+
35214caseinase-3.4.21.50
35214catalase+1.11.1.6
35214tween esterase+
35214gamma-glutamyltransferase+2.3.2.2
35214lecithinase-
35214lipase-
35214lysine decarboxylase-4.1.1.18
35214ornithine decarboxylase-4.1.1.17
35214urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35214-+++-+++-+++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35214---------------------+/-------------------------+/---

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinent
35214USAUSANorth America
35214United States of AmericaUSANorth America

Safety information

risk assessment

  • @ref: 35214
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35214

culture collection no.: CIP 106828, NRRL NRS-800

straininfo link

  • @ref: 93199
  • straininfo: 67445

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35214Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106828Collection of Institut Pasteur (CIP 106828)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
93199Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67445.1