Strain identifier

BacDive ID: 1359

Type strain: Yes

Species: Lentibacillus salicampi

Strain Designation: SF-20

Strain history: CIP <- 2003, JCM <- 2001, Y. H. Park: strain SF-20

NCBI tax ID(s): 175306 (species)

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General

@ref: 6445

BacDive-ID: 1359

DSM-Number: 16425

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-variable, motile, rod-shaped, colony-forming

description: Lentibacillus salicampi SF-20 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from salt field.

NCBI tax id

  • NCBI tax id: 175306
  • Matching level: species

strain history

@refhistory
6445<- CIP <- Y.-H. Park; SF-20
67770Y.-H. Park SF-20.
122372CIP <- 2003, JCM <- 2001, Y. H. Park: strain SF-20

doi: 10.13145/bacdive1359.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus salicampi
  • full scientific name: Lentibacillus salicampi Yoon et al. 2002

@ref: 6445

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus salicampi

full scientific name: Lentibacillus salicampi Yoon et al. 2002

strain designation: SF-20

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
22996variable2.0-4.0 µm0.4-0.7 µmrod-shapedyesmonotrichous
122372positiverod-shaped

colony morphology

  • @ref: 22996
  • colony size: 1.0-2.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 7 days
  • medium used: MA supplemented with 3-1% NaCl

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6445BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
22996MA supplemented with 3-1% NaClyes
42171MEDIUM 465 - for Lentibacillus salicampiyesDistilled water make up to (1000.000 ml);Sodium chloride (31.000 g);Marine agar (55.100 g)
122372CIP Medium 465yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=465

culture temp

@refgrowthtypetemperaturerange
6445positivegrowth30mesophilic
22996positivegrowth15.0psychrophilic
22996positivegrowth40.0thermophilic
22996nogrowth10.0psychrophilic
22996nogrowth>41.0thermophilic
42171positivegrowth30mesophilic
67770positivegrowth30mesophilic
122372positivegrowth22-37
122372nogrowth10psychrophilic
122372nogrowth41thermophilic
122372nogrowth45thermophilic

culture pH

@refabilitytypepH
22996positiveoptimum6.0-8.0
22996nogrowth5.0
122372nogrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 22996
  • oxygen tolerance: aerobe

spore formation

  • @ref: 22996
  • spore description: spherical or oval,terminal,in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refsalttested relationconcentrationgrowthhalophily level
22996NaCloptimum4.0-8.0 %(w/v)
22996NaClgrowth23.0 %positive
22996NaClgrowth>24.0 %no
22996moderately halophilic
122372NaClgrowth2 %positive
122372NaClgrowth0 %no
122372NaClgrowth4 %no
122372NaClgrowth6 %no
122372NaClgrowth8 %no
122372NaClgrowth10 %no

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2299615963ribitol-builds acid from
2299617057cellobiose-builds acid from
2299615824D-fructose-builds acid from
2299612936D-galactose-builds acid from
2299617634D-glucose-builds acid from
2299616899D-mannitol-builds acid from
2299616024D-mannose-builds acid from
229966731melezitose-builds acid from
2299616634raffinose-builds acid from
2299616988D-ribose-builds acid from
2299617924D-sorbitol-builds acid from
2299616551D-trehalose-builds acid from
2299665327D-xylose-builds acid from
2299630849L-arabinose-builds acid from
2299662345L-rhamnose-builds acid from
2299617716lactose-builds acid from
2299617306maltose-builds acid from
2299628053melibiose-builds acid from
2299617268myo-inositol-builds acid from
2299617992sucrose-builds acid from
229964853esculin-hydrolysis
2299617368hypoxanthine-hydrolysis
2299628017starch-hydrolysis
2299618186tyrosine-hydrolysis
2299615318xanthine-hydrolysis
2299617164stachyose+builds acid from
22996casein+hydrolysis
2299653426tween 80+hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12237216947citrate-carbon source
1223724853esculin-hydrolysis
122372606565hippurate+hydrolysis
12237217632nitrate+reduction
12237216301nitrite-reduction

metabolite production

  • @ref: 122372
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12237215688acetoin-
12237217234glucose-

enzymes

@refvalueactivityec
22996catalase+1.11.1.6
22996cytochrome oxidase+1.9.3.1
22996urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
122372oxidase+
122372beta-galactosidase-3.2.1.23
122372alcohol dehydrogenase-1.1.1.1
122372gelatinase-
122372catalase+1.11.1.6
122372gamma-glutamyltransferase+2.3.2.2
122372lysine decarboxylase-4.1.1.18
122372ornithine decarboxylase-4.1.1.17
122372urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122372--+--++----------+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122372---------+/---------------------------------------+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment culture
22996salt fieldYellow SeaRepublic of KoreaKORAsiamarine agar (MA) (Difco) supplemented with 8 - 1% (w/v) NaCl
6445salt fieldYellw seaRepublic of KoreaKORAsia
67770Salt field of the Yellow SeaRepublic of KoreaKORAsia
122372Environment, Salt field of the Yellow seaYellow seaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2
#Environmental#Terrestrial
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_68523.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3088;97_31504;98_48241;99_68523&stattab=map
  • Last taxonomy: Lentibacillus salicampi subclade
  • 16S sequence: AY057394
  • Sequence Identity:
  • Total samples: 81
  • soil counts: 14
  • aquatic counts: 41
  • animal counts: 25
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64451Risk group (German classification)
1223721Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6445
  • description: Lentibacillus salicampi 16S ribosomal RNA gene, partial sequence
  • accession: AY057394
  • length: 1521
  • database: ena
  • NCBI tax ID: 175306

Genome sequences

  • @ref: 67770
  • description: Lentibacillus salicampi ATCC BAA-719
  • accession: GCA_004565465
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 175306

GC content

  • @ref: 67770
  • GC-content: 44
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes76.83no
flagellatedyes79.401yes
gram-positiveyes80.867yes
anaerobicno99.153yes
aerobicyes92.17yes
halophileyes94.889yes
spore-formingyes91.904yes
glucose-utilyes88.672no
thermophileno97.282no
glucose-fermentno84.239no

External links

@ref: 6445

culture collection no.: DSM 16425, CIP 107807, JCM 11462, KCCM 41560, ATCC BAA 719, CGMCC 1.3447, CGMCC 1.6154

straininfo link

  • @ref: 71010
  • straininfo: 100721

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12508866Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea.Yoon JH, Kang KH, Park YHInt J Syst Evol Microbiol10.1099/00207713-52-6-20432002Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Geologic Sediments/microbiology, Korea, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium ChlorideGenetics
Phylogeny15879279Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus.Jeon CO, Lim JM, Lee JC, Lee GS, Lee JM, Xu LH, Jiang CL, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.63462-02005Bacillaceae/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Flagella/physiology, Genes, Bacterial, Genes, rRNA, Gentian Violet, Geologic Sediments/*microbiology, Growth Inhibitors/pharmacology, Molecular Sequence Data, Movement, Phenazines, Phylogeny, Quinones/analysis/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/pharmacology, *Soil Microbiology, Spores, Bacterial, TemperatureEnzymology
Phylogeny16166670Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China.Lim JM, Jeon CO, Song SM, Lee JC, Ju YJ, Xu LH, Jiang CL, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.63721-02005Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium ChlorideGenetics
Phylogeny19671718Lentibacillus persicus sp. nov., a moderately halophilic species isolated from a saline lake.Sanchez-Porro C, Amoozegar MA, Fernandez AB, Babavalian Fard H, Ramezani M, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.015396-02009Bacillaceae/classification/cytology/*genetics/isolation & purification, DNA Primers, DNA, Bacterial/chemistry/genetics, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Water MicrobiologyGenetics
Phylogeny26545789Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample.Wang JL, Ma KD, Wang YW, Wang HM, Li YB, Zhou S, Chen XR, Kong DL, Guo X, He MX, Ruan ZYAntonie Van Leeuwenhoek10.1007/s10482-015-0618-92015Bacillaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/analysis/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/analysis/*metabolismMetabolism
Genetics31320435Genome Sequence of the Moderately Halophilic Yellow Sea Bacterium Lentibacillus salicampi ATCC BAA-719(T).Simoes Junior M, MacLea KSMicrobiol Resour Announc10.1128/MRA.00702-192019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6445Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16425)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16425
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22996Jung-Hoon Yoon,Kook Hee Kang,Yong-Ha Park10.1099/00207713-52-6-2043Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea.IJSEM 52: 2043-2048 200212508866
42171Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5284
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71010Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100721.1StrainInfo: A central database for resolving microbial strain identifiers
122372Curators of the CIPCollection of Institut Pasteur (CIP 107807)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107807