Strain identifier

BacDive ID: 13551

Type strain: Yes

Species: Ensifer saheli

Strain history: CIP <- 2002, P. De Lajudie, Montpellier, France: strain ORS 609T <- 1987, B. Dreyfus, ORSTOM, Dakar, Senegal (Rhizobium sp.) <- I. Ndoye ORSTOM, Dakar, Senegal

NCBI tax ID(s): 36856 (species)

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General

@ref: 4275

BacDive-ID: 13551

DSM-Number: 11273

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile

description: Ensifer saheli DSM 11273 is a mesophilic, motile bacterium that was isolated from rhizosphere of Sesbania.

NCBI tax id

  • NCBI tax id: 36856
  • Matching level: species

strain history

@refhistory
4275<- LMG <- B. Dreyfus, Laboratoire de Mikrobiologie des Sols, Dakar <- I. Noloye, Laboratoire de Mikrobiologie des Sols, Dakar
122349CIP <- 2002, P. De Lajudie, Montpellier, France: strain ORS 609T <- 1987, B. Dreyfus, ORSTOM, Dakar, Senegal (Rhizobium sp.) <- I. Ndoye ORSTOM, Dakar, Senegal

doi: 10.13145/bacdive13551.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Rhizobiaceae
  • genus: Ensifer
  • species: Ensifer saheli
  • full scientific name: Ensifer saheli (De Lajudie et al. 1994) Young 2003

  • synonyms

    • @ref: 20215
    • synonym: Sinorhizobium saheli

@ref: 4275

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Rhizobiaceae

genus: Ensifer

species: Ensifer saheli

full scientific name: Ensifer saheli (De Lajudie et al. 1994) Young 2003

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.124
6948099.996negative
122349nonegativerod-shaped

colony morphology

  • @ref: 122349

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4275RHIZOBIUM MEDIUM (DSMZ Medium 98)yeshttps://mediadive.dsmz.de/medium/98Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
33112MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
122349CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
4275positivegrowth30mesophilic
33112positivegrowth30mesophilic
122349positivegrowth25-41
122349nogrowth10psychrophilic
122349nogrowth45thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.994

halophily

@refsaltgrowthtested relationconcentration
122349NaClpositivegrowth0 %
122349NaClnogrowth2 %
122349NaClnogrowth4 %
122349NaClnogrowth6 %
122349NaClnogrowth8 %
122349NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose+builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122349citrate-carbon source16947
122349esculin-hydrolysis4853
122349hippurate-hydrolysis606565
122349nitrate-reduction17632
122349nitrite-reduction16301

antibiotic resistance

  • @ref: 122349
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 122349
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12234915688acetoin-
12234917234glucose-

enzymes

@refvalueactivityec
122349oxidase+
122349beta-galactosidase+3.2.1.23
122349alcohol dehydrogenase-1.1.1.1
122349gelatinase-
122349catalase+1.11.1.6
122349lysine decarboxylase-4.1.1.18
122349ornithine decarboxylase-4.1.1.17
122349phenylalanine ammonia-lyase-4.3.1.24
122349tryptophan deaminase-
122349urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122349-++--++-+-++-+-+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122349---+--------------------+-----------------+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122349+++++-+++++++++++++++++++-+----+++++++----------++-+-------+--+-------++---+---++-----+--+++-+-+-+-

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
4275rhizosphere of SesbaniaSesbaniaSenegalSENAfrica
122349Sesbania cannabinaSenegalSENAfrica

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_273.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_84;97_88;98_232;99_273&stattab=map
  • Last taxonomy: Rhizobiaceae
  • 16S sequence: X68390
  • Sequence Identity:
  • Total samples: 9487
  • soil counts: 5353
  • aquatic counts: 1562
  • animal counts: 1094
  • plant counts: 1478

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
42751Risk group (German classification)
1223491Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sinorhizobium saheli strain LMG 7837 16S ribosomal RNA gene, partial sequence; internal transcribed spacer, complete sequence; and 23S ribosomal RNA, partial sequenceAF3452871082ena36856
20218Sinorhizobium saheli partial 16S rRNA gene, strain LMG 7837X683901437ena36856
20218Sinorhizobium saheli gene for 16S rRNA, partial sequence, strain: NBRC 100386AB6811611410ena36856

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sinorhizobium saheli LMG 7837GCA_001651875scaffoldncbi36856
66792Sinorhizobium saheli strain LMG 783736856.3wgspatric36856
66792Sinorhizobium saheli LMG 78372751185839draftimg36856

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno75.499no
gram-positiveno98.104no
anaerobicno98.365no
halophileno92.208no
spore-formingno94.296no
glucose-utilyes93.033no
motileyes86.621no
aerobicyes92.453no
thermophileno98.754no
glucose-fermentno91.135no

External links

@ref: 4275

culture collection no.: DSM 11273, LMG 7837, ORS 609, CIP 107336, ATCC 51690, ORS 609T

straininfo link

  • @ref: 82740
  • straininfo: 4803

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9464375Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America.Haukka K, Lindstrom K, Young JPAppl Environ Microbiol10.1128/AEM.64.2.419-426.19981998Acyltransferases/*genetics, Bacterial Proteins, Fabaceae/*microbiology, Gene Dosage, *Genes, Bacterial, Nitrogenase/*genetics, *Oxidoreductases, Phylogeny, *Plants, Medicinal, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Rhizobium/*classification/genetics, SymbiosisEnzymology
Phylogeny27653171Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline-alkaline soils.Li Y, Yan J, Yu B, Wang ET, Li X, Yan H, Liu W, Xie ZInt J Syst Evol Microbiol10.1099/ijsem.0.0015102016Alkalies, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizobiaceae/*classification/genetics/isolation & purification, Root Nodules, Plant/*microbiology, Salinity, Sequence Analysis, DNA, Sesbania/*microbiology, Soil/chemistry, SymbiosisTranscriptome
29155157Synthesis, characterization, and the antifungal activity of chitosan derivatives containing urea groups.Zhang J, Tan W, Zhang Z, Song Y, Li Q, Dong F, Guo ZInt J Biol Macromol10.1016/j.ijbiomac.2017.11.0922017Antifungal Agents/chemical synthesis/*chemistry/*pharmacology, Chemistry Techniques, Synthetic, Chitosan/chemical synthesis/*chemistry/*pharmacology, Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Solubility, Spectroscopy, Fourier Transform Infrared, Structure-Activity Relationship, Urea/*chemistry

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4275Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11273)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11273
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33112Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4760
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82740Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4803.1StrainInfo: A central database for resolving microbial strain identifiers
122349Curators of the CIPCollection of Institut Pasteur (CIP 107336)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107336